Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,559,357 Δ1,629 bp [rtcR][glpG] [rtcR], glpR, glpR, [glpG]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3559357 3560985 1629 48 [1] [0] 48 [rtcR]–[glpG] [rtcR],glpR,glpR,[glpG]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 35593561 (0.020)45 (1.100) 35/244 0.3 98.9% coding (1089/1599 nt) rtcR sigma 54‑dependent transcriptional regulator of rtcBA expression
?NC_000913 3560986 = 0 (0.000)coding (467/831 nt) glpG rhomboid intramembrane serine protease

TACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAACAC  >  NC_000913/3560839‑3561134
                                                                                                                                                   |                                                                                                                                                    
ggcagcgatcgccaggtgagcagtgattttcagcttatcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccaga                                                                                                                                                      <  2:77600‑M2/1‑1 (MQ=255)
 gcagcgatcgccaggtgagcagtgattttcagcttatcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagag                                                                                                                                                     >  8:59251‑M2/147‑148 (MQ=255)
        tcgccaggtgagcagtgattttcagcttatcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTa                                                                                                                                              >  5:15753‑M2/140‑148 (MQ=255)
                    cagtgattttcagcttatcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCa                                                                                                                                 >  7:74860‑M2/128‑149 (MQ=255)
                           tttcagcttatcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGc                                                                                                                            >  2:43301‑M2/121‑147 (MQ=255)
                               agcttatcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAg                                                                                                                      >  3:151010‑M2/117‑149 (MQ=255)
                                gcttatcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGt                                                                                                                     >  2:209920‑M2/116‑149 (MQ=255)
                                    atcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGc                                                                                                                   <  1:43301‑M2/36‑1 (MQ=255)
                                    atcgccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGc                                                                                                                   <  4:151010‑M2/36‑1 (MQ=255)
                                        ccggaacggtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAAc                                                                                                             <  8:74860‑M2/42‑1 (MQ=255)
                                                gtgcgcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGt                                                                                                     <  3:4620‑M2/50‑1 (MQ=255)
                                                    gcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccaga                                                                                                                                                      <  4:133455‑M2/1‑1 (MQ=255)
                                                    gcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccaga                                                                                                                                                      >  3:133455‑M2/96‑96 (MQ=255)
                                                    gcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAAcgc                                                                                                           <  3:147761‑M2/44‑1 (MQ=255)
                                                    gcgatttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAAcgc                                                                                                           >  4:147761‑M2/96‑139 (MQ=255)
                                                        tttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGatat                                                                                                                                    >  3:106496‑M2/92‑110 (MQ=255)
                                                        tttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGatat                                                                                                                                    <  4:106496‑M2/19‑1 (MQ=255)
                                                        tttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGc                                                                                                                            >  2:50771‑M2/92‑118 (MQ=255)
                                                        tttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGc                                                                                                                            <  1:50771‑M2/27‑1 (MQ=255)
                                                         tttgcgccagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggacctcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCAGCAGCGAGCAGTGCATTAACGCGTGGGTGAAGTAACg                                                                                            >  4:29746‑M2/91‑149 (MQ=255)
                                                               cagctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGaa                                                                                      <  6:15753‑M2/65‑1 (MQ=255)
                                                                  ctggttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTc                                                                                   <  1:209920‑M2/68‑1 (MQ=255)
                                                                      ttgccgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAAt                                                                               <  7:59251‑M2/72‑1 (MQ=255)
                                                                          cgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAGttt                                                                             >  5:183771‑M2/74‑147 (MQ=255)
                                                                          cgaaggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAAttt                                                                             >  5:38549‑M2/74‑147 (MQ=255)
                                                                             aggcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAgtg                                                                        >  4:38184‑M2/71‑149 (MQ=255)
                                                                               gcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAaga                                                                                                                                          <  3:124586‑M2/13‑1 (MQ=255)
                                                                               gcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAaga                                                                                                                                          >  4:124586‑M2/69‑81 (MQ=255)
                                                                               gcaaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGtt                                                                      >  2:178442‑M2/69‑149 (MQ=255)
                                                                                 aaatttcgcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGaa                                                                                                                       >  6:1722‑M2/67‑98 (MQ=255)
                                                                                        gcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAaga                                                                                                                                          >  4:55572‑M2/60‑72 (MQ=255)
                                                                                        gcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAaga                                                                                                                                          <  3:55572‑M2/13‑1 (MQ=255)
                                                                                        gcgaagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGaa                                                              <  1:178442‑M2/89‑1 (MQ=255)
                                                                                         gcgaagatctgtacgcgcggatcaatctctggacctcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGAGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATg                                                            <  3:29746‑M2/91‑1 (MQ=255)
                                                                                           aagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGaa                                                                                      <  5:6841‑M2/65‑1 (MQ=255)
                                                                                           aagatctgtacgcgcggatcaatctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGaa                                                                                      >  6:6841‑M2/57‑121 (MQ=255)
                                                                                                                atctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATc                                                                 >  4:105493‑M2/36‑121 (MQ=255)
                                                                                                                atctctggaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATc                                                                 <  3:105493‑M2/86‑1 (MQ=255)
                                                                                                                       gaccttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCt                                 <  6:183771‑M2/118‑1 (MQ=255)
                                                                                                                          cttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGc                           >  2:177338‑M2/26‑149 (MQ=255)
                                                                                                                          cttcaccctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGc                           >  1:217408‑M2/26‑149 (MQ=255)
                                                                                                                                cctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCATTTcc                                  <  5:86187‑M2/117‑1 (MQ=255)
                                                                                                                                cctgccgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCATTTcc                                  >  6:86187‑M2/20‑136 (MQ=255)
                                                                                                                                     cgggtctacgccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGc                <  6:38549‑M2/135‑1 (MQ=255)
                                                                                                                                              gccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAAt        >  5:50223‑M2/6‑148 (MQ=255)
                                                                                                                                               ccagAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATa       >  5:55355‑M2/5‑148 (MQ=255)
                                                                                                                                                 agAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAac    <  2:217408‑M2/147‑1 (MQ=255)
                                                                                                                                                   agCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAacac  <  6:50223/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCCACCGCACCGCCGAGATACCACCACCAGAGCAGGTTAAAGAGGATATGCATCAGCGAGAAGTGCATTAACGCGTGGGTGAAGTAACGCCAGAACTCAAATTTCAGTGTTGGATCGAATGGCCAGGCCAGCCATAACATCACTTCCTGATCGCCGAGAATTTGCATGGCAATAAACAC  >  NC_000913/3560839‑3561134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.