| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,606,239 | A→G | 15.4% | noncoding (48/87 nt) | leuP ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,239 | 0 | A | G | 15.4% | 83.4 / 9.0 | 40 | noncoding (48/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base A (17/16); new base G (3/3); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACA > NC_000913/4606095‑4606357 | ccgacctcccccACGTCCGTAAGGACACTAACACCTGAACCAAGCGCGTCTTCAAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGTTATCTCCTCTTTTGGTGCGTTGGGGGTGACTTGAACCCCCACGTCCGTAAGAaca < 2:326127/141‑1 (MQ=21) ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca > 1:326127/1‑140 (MQ=255) tAAAACCTGAAGATATCTAGTATACAAAATCCGCCACCTTATCACAGTCATCTAACTTTTTTTGATATCTCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa < 7:129219/149‑1 (MQ=18) tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATGGCCTCGTTTGGTGCGAGGGGGGGGACTAGAACCCCCACGTCCGTAAGATCTCAAACGCCTGAAGCTCACGCGGCGGCCAAtatcgc > 1:41898/1‑143 (MQ=37) gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCCTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTGGACCCCCCACGTCCGGAAGAACACTAAAAAGAGCAGCAGGCGCCTGTTCAAATACCGCCACCtatcg > 3:326525/1‑145 (MQ=25) aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt < 4:4772/127‑1 (MQ=255) aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt > 3:4772/1‑127 (MQ=255) ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa > 1:79302/1‑94 (MQ=255) ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa < 2:79302/94‑1 (MQ=255) ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACAACGAACTGGAGCTCGCGGGACGACCAGTACCGCCACATTAGCCCAGTggcc > 7:97243/1‑146 (MQ=25) ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc > 3:295789/1‑128 (MQ=255) ttCCGCATCCTTCGCACACGCACAGTTCTTTATTTGATATCGCGTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc < 4:295789/128‑1 (MQ=14) ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACCCATGAAGCTAGCGCGTCGACCAATGCCGCCACCTTCGCCCAGTgcgcgccttagca > 7:403456/1‑142 (MQ=21) ccACCTTCGCACAGTCATCTTACTTTTTTAGATATCGCCTCGTATGGTGCGAGCGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGACAACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAAATc > 8:93312/1‑148 (MQ=21) aGTCATCTTACTTTTTTTTATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg < 7:93312/149‑1 (MQ=255) cTTACTTTTTTTGATAACGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa < 4:289074/67‑1 (MQ=255) cTTACTTTTTTTGATAACGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa > 3:289074/1‑67 (MQ=255) aCTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa < 2:376126/95‑1 (MQ=255) aCTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa > 1:376126/1‑95 (MQ=255) tttttGATATCGCCTCGTTTTGTGAGAGGGGGGGGACTTGCACACCCACGTCCGTAAGAACACTAACACCTGACGCTAGCGCGTCTACCACTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg < 8:403456/147‑1 (MQ=255) cTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc < 8:14172/90‑1 (MQ=255) cTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc > 7:14172/1‑90 (MQ=255) attGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 6:332149/89‑1 (MQ=18) tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTCGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGAGGACTTGa < 8:363434/134‑1 (MQ=255) tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGAGGACTTGa > 7:363434/1‑134 (MQ=255) gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc > 5:223841/1‑100 (MQ=255) gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc < 6:223841/100‑1 (MQ=255) gggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg < 7:401335/75‑1 (MQ=34) gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt < 3:380158/79‑1 (MQ=39) gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt > 4:380158/1‑79 (MQ=39) aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg < 2:340024/79‑1 (MQ=21) aCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg > 1:340024/1‑79 (MQ=21) ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa < 6:14394/78‑1 (MQ=25) ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa > 5:14394/1‑78 (MQ=25) ttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa < 7:289771/114‑1 (MQ=255) ttGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGa > 8:289771/1‑114 (MQ=255) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 4:223015/1‑67 (MQ=35) gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc < 3:223015/67‑1 (MQ=35) cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg < 7:221654/74‑1 (MQ=255) cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg > 8:221654/1‑74 (MQ=255) ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca < 7:150763/62‑2 (MQ=18) ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca > 8:150763/1‑61 (MQ=18) ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAAGaca > 2:148073/1‑132 (MQ=255) ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAAGaca < 1:148073/132‑1 (MQ=255) | ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACA > NC_000913/4606095‑4606357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |