Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,793,741 T→C 5.2% L336P (CTG→CCG)  gabT → 4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,793,7410TC 5.2% 235.5 / 2.4 76L336P (CTG→CCG) gabT4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (36/36);  new base C (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCT  >  NC_000913/2793593‑2793888
                                                                                                                                                    |                                                                                                                                                   
gATGCCGTCGCTCCAGGCGGTGTGGGCGGAATCTATTGTGGTAACGCTATTGCCTGGGTGGCTGCGCTGGAAGTGTTGAAGGTTTTTGAGCAGTAAAATCTGCTGCAAAATTCCAACGATGTGGTGCAGAATTTGAAAGACGTATTGCt                                                                                                                                                     <  8:364548/149‑1 (MQ=255)
gATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCGGCGTGGCTGCGCGGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGAGCTGGGGCAGAAGTTGAAAGACGGATTGCt                                                                                                                                                     >  4:58885/1‑149 (MQ=255)
    ccGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCg                                                                                                                                                 <  2:336146/149‑1 (MQ=255)
     cgccGCTCCCGGCGGTCTGGGGGGCACCAATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGGGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGa                                                                                                                                                <  8:420159/146‑1 (MQ=255)
           tCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGcc                                                                                                                                           >  3:368413/1‑148 (MQ=255)
             cAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGa                                                                                                                                         >  6:105145/1‑148 (MQ=255)
               ggCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTTCAAAAAGCCACAGATCTGGGTCAGAAGTTGAAAGACGGATAGCTGGAGATAGCCGaaa                                                                                                                                       >  1:309592/1‑148 (MQ=255)
                      tGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCg                                                                                                                               >  8:176460/1‑149 (MQ=255)
                        ggcggcACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACAccc                                                                                                                                <  4:371645/146‑1 (MQ=255)
                             cACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCg                                                                                                                        <  6:301058/149‑1 (MQ=255)
                                cTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAATACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGc                                                                                                                      >  6:389117/1‑148 (MQ=255)
                                       ggTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGt                                                                                                                 <  8:4754/146‑1 (MQ=255)
                                       ggTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGt                                                                                                                 >  7:4754/1‑146 (MQ=255)
                                        gTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTAcgc                                                                                                             >  4:294544/1‑149 (MQ=255)
                                           aCCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCgg                                                                                                           <  7:176460/148‑1 (MQ=255)
                                            cccGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGc                                                                                                         >  4:268856/1‑149 (MQ=255)
                                             ccGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCCGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCt                                                                                                        >  5:144778/1‑149 (MQ=255)
                                             ccGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCCGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCt                                                                                                        >  5:150948/1‑149 (MQ=255)
                                              cGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTg                                                                                                       <  1:110606/149‑1 (MQ=255)
                                                     cTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCga                                                                                                >  3:322299/1‑149 (MQ=255)
                                                     cTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCga                                                                                                >  1:137664/1‑149 (MQ=255)
                                                        cGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgatga                                                                                             >  6:221226/1‑149 (MQ=255)
                                                         gTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgatgat                                                                                            <  8:69304/149‑1 (MQ=255)
                                                          tGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATc                                                                                           <  2:137664/149‑1 (MQ=255)
                                                                 gcTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCAtt                                                                                     >  8:423224/1‑148 (MQ=255)
                                                                   tGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAg                                                                                  >  5:11724/1‑149 (MQ=255)
                                                                   gggAAGTGGTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAg                                                                                  <  8:164264/148‑1 (MQ=255)
                                                                       aGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGt                                                                              <  3:294544/149‑1 (MQ=255)
                                                                        gtgtTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGGAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGtt                                                                             <  7:250122/149‑1 (MQ=255)
                                                                        gtgtTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGtt                                                                             >  6:30328/1‑149 (MQ=255)
                                                                         tggtGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGttt                                                                            <  5:221226/146‑1 (MQ=255)
                                                                           ttGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAATAAGGATTGCTGGCTATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATGGAGCTGATTg                                                                           >  1:298187/1‑148 (MQ=255)
                                                                           ttGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCAtt                                                                                     >  8:327896/1‑138 (MQ=255)
                                                                           ttGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCAtt                                                                                     <  7:327896/138‑1 (MQ=255)
                                                                           ttGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCAt                                                                                      >  1:389124/1‑137 (MQ=255)
                                                                           ttGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCAt                                                                                      <  2:389124/137‑1 (MQ=255)
                                                                             gAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTg                                                                           >  3:386794/1‑146 (MQ=255)
                                                                              aaGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGCCGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGa                                                                       >  2:82262/1‑149 (MQ=255)
                                                                              aaGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTg                                                                           >  7:241115/1‑145 (MQ=255)
                                                                              aaGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTg                                                                           <  8:241115/145‑1 (MQ=255)
                                                                                ggTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgat                                                                                               <  6:50879/123‑1 (MQ=255)
                                                                                ggTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCgat                                                                                               >  5:50879/1‑123 (MQ=255)
                                                                                   gtTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGCTCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAATACACGCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACCGc                                                                   >  5:302989/1‑148 (MQ=255)
                                                                                    tttGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCg                                                                  <  5:238531/148‑1 (MQ=255)
                                                                                       gAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCTAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATc                                                               <  5:250247/148‑1 (MQ=255)
                                                                                        aGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGc                                                                                                  <  5:301851/112‑1 (MQ=255)
                                                                                        aGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGc                                                                                                  >  6:301851/1‑112 (MQ=255)
                                                                                        aGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGaa                                                                         <  4:236165/137‑1 (MQ=255)
                                                                                        aGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGaa                                                                         >  3:236165/1‑137 (MQ=255)
                                                                                        aGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCCGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATca                                                              <  6:150948/148‑1 (MQ=255)
                                                                                        aGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCCGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATca                                                              <  6:144778/148‑1 (MQ=255)
                                                                                           aGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTTAGCTGTTTGAAGACGGCGATCacaac                                                          <  5:36489/149‑1 (MQ=255)
                                                                                            ggAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCacaaca                                                         <  3:268856/149‑1 (MQ=255)
                                                                                             gAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGTCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACTCGGGCTGGGTGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCAcaacaa                                                        <  3:58885/149‑1 (MQ=255)
                                                                                                  tCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCgg                                                   >  8:229068/1‑149 (MQ=255)
                                                                                                  tCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCg                                                    >  4:382637/1‑148 (MQ=255)
                                                                                                    tgcttcAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGAc                                                 <  2:309592/149‑1 (MQ=255)
                                                                                                      ctgcAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGAc                                                 <  7:229068/147‑1 (MQ=255)
                                                                                                         cAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCa                                             >  3:178608/1‑148 (MQ=255)
                                                                                                          aaaaaGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGACGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCaaa                                           <  5:105145/149‑1 (MQ=255)
                                                                                                                 cAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCa                                             <  6:67552/140‑1 (MQ=255)
                                                                                                                 cAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCa                                             >  5:67552/1‑140 (MQ=255)
                                                                                                                 cAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCAccg                                    >  7:350039/1‑149 (MQ=255)
                                                                                                                  aaCGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCAccgc                                   >  6:358902/1‑149 (MQ=255)
                                                                                                                   aCGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCAccgcc                                  <  6:11724/149‑1 (MQ=255)
                                                                                                                    cGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTg                                                                                    >  1:363074/1‑98 (MQ=255)
                                                                                                                    cGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTg                                                                                    <  2:363074/98‑1 (MQ=255)
                                                                                                                          ggggCAGAAGTTGAAAGACGGATTGCTGGCTATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCg                                                                                                 <  3:26993/79‑1 (MQ=255)
                                                                                                                          ggggCAGAAGTTGAAAGACGGATTGCTGGCTATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCg                                                                                                 >  4:26993/1‑79 (MQ=255)
                                                                                                                           gggCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGt                          >  8:355043/1‑149 (MQ=255)
                                                                                                                              cAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGc                       <  4:350570/149‑1 (MQ=255)
                                                                                                                                 aaGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcg                    >  4:222291/1‑149 (MQ=255)
                                                                                                                                  aGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcgc                   <  4:322299/149‑1 (MQ=255)
                                                                                                                                   gTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTcgcg                  <  7:423224/149‑1 (MQ=255)
                                                                                                                                    ttGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCGCCGCCGAGATCGTAGCTCgcgc                 >  3:261072/1‑149 (MQ=255)
                                                                                                                                       aaaGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCcg              <  5:30328/149‑1 (MQ=255)
                                                                                                                                          gACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGa           <  4:121184/149‑1 (MQ=255)
                                                                                                                                                   cTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCt  >  5:341078/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCGAAAAACACCCGGAGATCGGCGACGTACGCGGGCTGGGGGCGATGATCGCCATTGAGCTGTTTGAAGACGGCGATCACAACAAGCCGGACGCCAAACTCACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCT  >  NC_000913/2793593‑2793888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: