Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,863,802 C→A 6.0% intergenic (+36/‑48) gapA → / → yeaD glyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,8020CA 6.0% 367.4 / 6.0 107intergenic (+36/‑48)gapA/yeaDglyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (55/45);  new base A (3/4);  total (58/49)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCATCC  >  NC_000913/1863655‑1863950
                                                                                                                                                   |                                                                                                                                                    
tAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCg                                                                                                                                                     >  8:338729/1‑149 (MQ=255)
           tCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCtttt                                                                                                                                          >  5:242476/1‑149 (MQ=255)
            cGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                         <  1:285837/149‑1 (MQ=255)
             gCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                         <  5:124762/148‑1 (MQ=255)
              ctctGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                       >  2:51380/1‑149 (MQ=255)
              ctctGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                       >  4:189728/1‑149 (MQ=255)
                  gAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTAcc                                                                                                                                   >  3:271932/1‑149 (MQ=255)
                      gccAACTTCGTGAAACTGGTATCCTGGGACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTTAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGAt                                                                                                                               <  5:62901/147‑1 (MQ=255)
                         aaCTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                         <  8:169209/136‑1 (MQ=255)
                         aaCTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                         >  7:169209/1‑136 (MQ=255)
                          aCTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATaaaa                                                                                                                           >  8:184253/1‑149 (MQ=255)
                           cTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAAt                                                                                                                          >  1:195971/1‑149 (MQ=255)
                              cGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGa                                                                                                                        <  1:113750/148‑1 (MQ=255)
                               gTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                       <  7:338729/148‑1 (MQ=255)
                                 gAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGt                                                                                                                     >  1:366152/1‑148 (MQ=255)
                                 gAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGt                                                                                                                     >  1:334669/1‑148 (MQ=255)
                                        gTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGAc                                                                                                             >  4:50073/1‑149 (MQ=255)
                                           tCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGc                                                                                                          >  7:77297/1‑149 (MQ=255)
                                             cTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGt                                                                                                        >  4:226733/1‑149 (MQ=255)
                                                ttACGACAACGAAACCGTTTACTCCAACAAAGTTCTGGACCTGATGGCTCACATCTCAAAATAAGTTCAGATGACACTGTGATCTAAAAAGAGCGTGTTCGGTCGCTCTTTTTTTTAACTGATAAAATGAAGTTAAAGGACTGCGTCa                                                                                                      <  7:201233/147‑1 (MQ=255)
                                                gTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCa                                                                                                      <  6:242476/148‑1 (MQ=255)
                                                    gACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGAt                                                                                                  >  3:30403/1‑148 (MQ=255)
                                                    gACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGAtt                                                                                                 >  3:56649/1‑148 (MQ=255)
                                                      cAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttt                                                                                                                                           >  2:150952/1‑105 (MQ=255)
                                                      cAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGAtt                                                                                                 >  7:166413/1‑147 (MQ=255)
                                                       aaCGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGAtt                                                                                                 >  7:331661/1‑146 (MQ=255)
                                                           aaaCCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGaaaa                                                                                          <  2:366152/149‑1 (MQ=255)
                                                             aCCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAtt                                                                                        >  7:375733/1‑149 (MQ=255)
                                                                  ttACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGc                                                                                   <  7:113626/149‑1 (MQ=255)
                                                                   tACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGcc                                                                                  >  8:359492/1‑149 (MQ=255)
                                                                      tCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGAGTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCtt                                                                               >  6:154309/1‑149 (MQ=255)
                                                                      tCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGc                                                                                   >  1:376232/1‑145 (MQ=255)
                                                                      tCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGc                                                                                   <  2:376232/145‑1 (MQ=255)
                                                                      tCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCtt                                                                               >  1:294021/1‑149 (MQ=255)
                                                                        caacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                             <  6:8954/149‑1 (MQ=255)
                                                                        caacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                             <  2:345796/149‑1 (MQ=255)
                                                                        caacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTATTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                             >  3:223393/1‑149 (MQ=255)
                                                                        caacaaAGGTCTCGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGGCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTc                                                                              <  5:165749/148‑1 (MQ=255)
                                                                         aacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCg                                                                            <  1:51380/149‑1 (MQ=255)
                                                                          acaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCgg                                                                           >  8:401491/1‑149 (MQ=255)
                                                                           caaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGt                                                                          >  6:288008/1‑149 (MQ=255)
                                                                           caaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGt                                                                          >  1:198316/1‑149 (MQ=255)
                                                                            aaaGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGt                                                                          >  4:195590/1‑148 (MQ=255)
                                                                             aaGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCa                                                                        <  1:100804/149‑1 (MQ=255)
                                                                              aGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCAt                                                                       >  7:680/1‑149 (MQ=255)
                                                                              aGTTCTGGACCTGATCGCTAACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAATTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCAt                                                                       <  2:294021/149‑1 (MQ=255)
                                                                                       ccTGATCGCTCACATCTCCAAATAAGTTGAGATTACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTTATAAAATGAAGTTAAAGGCCGGCGTCATGATTATGAAAATTTTTTCCGTTCCGGTCTTCGAACAAAt                                                              >  7:97898/1‑149 (MQ=255)
                                                                                         tGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAAtct                                                            >  7:174822/1‑149 (MQ=255)
                                                                                            tCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAACATGAAGTTAAAGGACTGCGGCAGGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTccc                                                         <  3:5430/149‑1 (MQ=255)
                                                                                                tCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                       <  4:86610/83‑1 (MQ=255)
                                                                                                tCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaa                                                                                                                       >  3:86610/1‑83 (MQ=255)
                                                                                                tCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGt                                                     >  7:242706/1‑149 (MQ=255)
                                                                                                tCACATCTCAAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGt                                                     >  5:372767/1‑149 (MQ=255)
                                                                                                gcacaTCTCCAAATAAGTTGAGATGCCACTGTGCTCTAAACAGAGCGACTTAGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAACGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTccc                                                         <  4:95822/144‑1 (MQ=255)
                                                                                                  acaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCa                                                                                                      >  7:398030/1‑98 (MQ=255)
                                                                                                  acaTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCa                                                                                                      <  8:398030/98‑1 (MQ=255)
                                                                                                   caTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCct                                                  <  2:198316/149‑1 (MQ=255)
                                                                                                      ctcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAACTCTCCCCTGTCCtctc                                               >  4:120956/1‑149 (MQ=255)
                                                                                                       tcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTcc                                              >  7:419620/1‑149 (MQ=255)
                                                                                                         cAAATAAGTTGGGATGACACTGTGATCTAAAAAGAGCGACATCGGTAGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGCCATCGAACAAATCTCCCCTGTCCTCTccc                                             <  4:84899/148‑1 (MQ=255)
                                                                                                         cAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcg                                            >  2:244054/1‑149 (MQ=255)
                                                                                                           aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAACTGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAACTCTCCCCTGTCCTCTCCcgtc                                          <  4:271932/149‑1 (MQ=255)
                                                                                                           aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAAtctc                                                           <  3:354468/132‑1 (MQ=255)
                                                                                                           aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAAtctc                                                           >  4:354468/1‑132 (MQ=255)
                                                                                                                   gagaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTccc                                                         >  1:152669/1‑126 (MQ=255)
                                                                                                                   gagaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTccc                                                         <  2:152669/126‑1 (MQ=255)
                                                                                                                   gagaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACt                                   <  7:401491/148‑1 (MQ=255)
                                                                                                                    agaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTg                                  <  3:195590/148‑1 (MQ=255)
                                                                                                                    agaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                                <  4:56649/149‑1 (MQ=255)
                                                                                                                     gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                                <  5:288008/149‑1 (MQ=255)
                                                                                                                      aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAATGACTGCGTCATGAATAAGAAAATTTTTGCCCTTCAGGTCATCGAACAAATCAACCCTGTCCTCTCCCGTCGTAAACTGGAt                               >  4:273332/1‑149 (MQ=255)
                                                                                                                      aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGa                                <  2:334669/148‑1 (MQ=255)
                                                                                                                      aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGAt                               >  4:165262/1‑149 (MQ=255)
                                                                                                                      aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGAt                               >  4:327187/1‑149 (MQ=255)
                                                                                                                      aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGAt                               <  2:195971/149‑1 (MQ=255)
                                                                                                                      aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGAt                               >  6:198266/1‑149 (MQ=255)
                                                                                                                        gACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACt                                   >  5:30632/1‑143 (MQ=255)
                                                                                                                        gACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACt                                   <  6:30632/143‑1 (MQ=255)
                                                                                                                        gACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATg                              >  7:140380/1‑148 (MQ=255)
                                                                                                                          cacTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAAc                           <  8:77297/149‑1 (MQ=255)
                                                                                                                           acTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAACTCTCCCCTGTCGTCTCCCGTCGTAAACTGGATGAACt                          >  7:181627/1‑149 (MQ=255)
                                                                                                                            cTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTg                         <  8:419620/149‑1 (MQ=255)
                                                                                                                            cTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTg                         <  8:174822/149‑1 (MQ=255)
                                                                                                                             tctgATCTAAAAAGAGCGACTTCGGTCGCGCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTGCCCTACCGGTCATCGAACAAATCTCCCCTGTCCTCTCACGTCTTAAACTGGATGAACTGGa                       >  3:84963/3‑149 (MQ=255)
                                                                                                                                gATCTAAAAATAGCGACTTAGGTCTCTCTTTTTTTTACGTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGAc                      <  3:50073/148‑1 (MQ=255)
                                                                                                                                gATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGAcc                     >  7:394634/1‑149 (MQ=255)
                                                                                                                                  tCTAAAAAGAGCGACTTCCGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCt                    >  8:277060/1‑148 (MQ=255)
                                                                                                                                  aaaaaaaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTc                                                                              >  8:60793/4‑90 (MQ=37)
                                                                                                                                  aaaaaaaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTc                                                                              <  7:60793/87‑1 (MQ=37)
                                                                                                                                    tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt                 >  4:317349/1‑149 (MQ=255)
                                                                                                                                    tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt                 >  4:162833/1‑149 (MQ=255)
                                                                                                                                     aaaaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAtt                >  2:52282/1‑149 (MQ=255)
                                                                                                                                      aaaaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAtt                <  3:226733/148‑1 (MQ=255)
                                                                                                                                      aaaaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATtg               <  1:186098/149‑1 (MQ=255)
                                                                                                                                      aaaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGAc                      >  3:213480/1‑142 (MQ=255)
                                                                                                                                      aaaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGAc                      <  4:213480/142‑1 (MQ=255)
                                                                                                                                             cGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGa        >  2:103794/1‑149 (MQ=255)
                                                                                                                                              gACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGat       <  3:273332/149‑1 (MQ=255)
                                                                                                                                                cTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGatca     <  5:154309/149‑1 (MQ=255)
                                                                                                                                                   cGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCATcc  >  8:194365/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATCATCC  >  NC_000913/1863655‑1863950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: