Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,807,286	A→C	15.1%	Y306S (TAC→TCC)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,286	0	A	C	15.1%	219.0 / 35.3	93	Y306S (TAC→TCC)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base A (41/38);  new base C (8/6);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

AGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCAT  >  NC_000913/1807138‑1807431                                                                                                                                                     |                                                                                                                                                  aGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTa                                                                                                                                                   <  3:226371/149‑1 (MQ=255)  gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAc                                                                                                                                                  <  3:247257/149‑1 (MQ=255)      ggTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                                                                                                                                              >  5:173087/1‑149 (MQ=255)            cgcgATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGc                                                                                                                                         >  3:13662/1‑148 (MQ=255)               gATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCACGCTaa                                                                                                                                      >  7:422726/1‑148 (MQ=255)                   acacTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaa                                                                                                                                 <  7:273348/149‑1 (MQ=255)                     acTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                                                                                                                >  6:104994/1‑148 (MQ=255)                       tGAAACAGGCAGAAAATTCCTCTCTTGAAGAGATGGATCGTTAAGGCGTAGATCCGGGGAGTGAACCCCCACTAAATCAGGCAACACCACTTTGCACCCATCACGATACGCAAAAAATTTACGCTTACCTTTACCGCTAAAAAAAACaa                                                                                                                             >  4:255995/1‑148 (MQ=255)                         aaaCTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTc                                                                                                                           >  5:195538/1‑149 (MQ=255)                          aaCTGGCAGAAAATGCCTCTCTTGTAGTGATGGTTCGTTTTGGCGTAGCTGGGGGGAGTGCAGCCACACTGAATCTGGGAAGACGTGTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTcc                                                                                                                          <  7:61540/149‑1 (MQ=255)                          aaCTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTcc                                                                                                                          <  5:104994/149‑1 (MQ=255)                            caggCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTcccc                                                                                                                        >  4:273010/3‑149 (MQ=255)                            cTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCACGCTAACAAAAACATTcccc                                                                                                                        >  4:278226/1‑149 (MQ=255)                             tGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTcccc                                                                                                                        >  4:27182/1‑148 (MQ=255)                               gCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTccc                                                                                                                                           >  1:100915/1‑127 (MQ=255)                               gCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTccc                                                                                                                                           <  2:100915/127‑1 (MQ=255)                                 aGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCa                                                                                                                      >  6:5620/1‑146 (MQ=255)                                 aGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCa                                                                                                                      <  5:5620/146‑1 (MQ=255)                                  gAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCAt                                                                                                                  >  1:67190/1‑149 (MQ=255)                                     aaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgg                                                                                                               >  2:203908/1‑149 (MQ=255)                                      attccTCTCTTGAAGTGATGGTTCTTTTTGGCGTAGCTGCGGGTAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCTATAACAAAAACATTCCCCCAGCATTggg                                                                                                              <  3:278226/146‑1 (MQ=255)                                      aTGCCTCTCTTGACGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGGGCAGCGACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                                                                              <  2:57847/149‑1 (MQ=255)                                      aTGCCTCTCTTGAAGCGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                                                                              <  3:273010/149‑1 (MQ=255)                                       tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgggg                                                                                                             <  3:27182/149‑1 (MQ=255)                                       tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgggg                                                                                                             <  8:422726/149‑1 (MQ=255)                                         ccTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggggg                                                                                                            <  7:124052/148‑1 (MQ=255)                                               ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                                                                                        <  6:195538/146‑1 (MQ=255)                                               ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAAGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                                                                                        <  5:131189/146‑1 (MQ=255)                                                 gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCa                                                                                                                      <  8:89965/130‑1 (MQ=255)                                                 gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCa                                                                                                                      >  7:89965/1‑130 (MQ=255)                                                    gagaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCaa                                                                                                <  3:239553/149‑1 (MQ=255)                                                           ttCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCg                                                                                         <  4:170059/149‑1 (MQ=255)                                                            tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGTGGGAATCATCACCATCCTGTCgg                                                                                        >  3:230592/1‑149 (MQ=255)                                                            tCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCgg                                                                                        >  7:331943/1‑149 (MQ=255)                                                               ttttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCaa                                                                                     <  2:67190/149‑1 (MQ=255)                                                                    gCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGt                                                                                >  4:107184/1‑149 (MQ=255)                                                                      gTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGttt                                                                              <  8:331943/149‑1 (MQ=255)                                                                            gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGa                                                                        >  1:312264/1‑149 (MQ=255)                                                                              ggggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGGCGGCAACGCGTTTCTCCGACt                                                                      <  2:309706/149‑1 (MQ=255)                                                                                   gTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCt                                                                 >  1:18983/1‑149 (MQ=255)                                                                                    tGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAc                                                                                               >  6:35411/1‑118 (MQ=255)                                                                                    tGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAc                                                                                               <  5:35411/118‑1 (MQ=255)                                                                                      cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCa                                                               >  6:178696/1‑148 (MQ=255)                                                                                       aGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGa                                                                        <  3:158181/138‑1 (MQ=255)                                                                                       aGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGa                                                                        >  4:158181/1‑138 (MQ=255)                                                                                        gCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaaa                                                            <  5:178696/149‑1 (MQ=255)                                                                                         ccACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAg                                                           >  4:356857/1‑149 (MQ=255)                                                                                           acacTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTc                                                         <  6:190647/149‑1 (MQ=255)                                                                                             acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                       >  1:132651/1‑149 (MQ=255)                                                                                             acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                       >  3:148086/1‑149 (MQ=255)                                                                                             acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                       >  4:341149/1‑149 (MQ=255)                                                                                              cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTATACCGCTAACAAAAACATTCCCACAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCTACTATGCTCAAAAGTCAtg                                                      <  1:40491/149‑1 (MQ=255)                                                                                                 aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGa                                                   <  2:52378/149‑1 (MQ=255)                                                                                                       ggAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACa                                              >  1:238506/1‑148 (MQ=255)                                                                                                        gAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACa                                              >  7:80249/1‑147 (MQ=255)                                                                                                         aaCACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCCCCACCCAGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAg                                           >  2:311237/1‑149 (MQ=255)                                                                                                          acacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgg                                          >  5:210946/1‑149 (MQ=255)                                                                                                            acGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                                                                                                                >  8:125682/1‑61 (MQ=255)                                                                                                            acGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                                                                                                                <  7:125682/61‑1 (MQ=255)                                                                                                                cTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGaa                                    >  8:201789/1‑149 (MQ=255)                                                                                                                  gtgCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAAc                                   >  5:395253/1‑148 (MQ=255)                                                                                                                     cTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATg                               >  1:383656/1‑149 (MQ=255)                                                                                                                        ccATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGc                             >  3:70522/1‑148 (MQ=255)                                                                                                                        ccATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCT‑CGACTATGCTCaa                                                              <  2:293862/114‑1 (MQ=255)                                                                                                                         cATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGc                             >  6:145685/1‑147 (MQ=255)                                                                                                                           tGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGt                         <  6:395253/149‑1 (MQ=255)                                                                                                                           tGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGt                         >  2:357724/1‑149 (MQ=255)                                                                                                                           tGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGt                         >  7:134002/1‑149 (MQ=255)                                                                                                                            gACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGCCTATGCTCAAAAGTCATGTGATAACAAACGGGTGAACTATGGCCATTg                        >  5:45787/1‑149 (MQ=255)                                                                                                                               gATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGc                      >  2:261737/1‑148 (MQ=255)                                                                                                                                 tACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGAt                   <  5:145685/149‑1 (MQ=255)                                                                                                                                   cGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATc                  <  3:255995/148‑1 (MQ=255)                                                                                                                                    gCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCtt                >  7:110143/1‑149 (MQ=255)                                                                                                                                    gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGa                    >  8:238257/1‑145 (MQ=255)                                                                                                                                    gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGa                    <  7:238257/145‑1 (MQ=255)                                                                                                                                    gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCtt                <  6:210946/149‑1 (MQ=255)                                                                                                                                    gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCtt                <  1:203908/149‑1 (MQ=255)                                                                                                                                      aaaaaaTTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTATCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTTAACTATCGCAAGTGGAGATCTTGt              >  8:164184/1‑149 (MQ=255)                                                                                                                                      aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGAt                                                  <  7:387979/113‑1 (MQ=255)                                                                                                                                      aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGt              <  4:148086/149‑1 (MQ=255)                                                                                                                                       aaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTc             <  2:132651/149‑1 (MQ=255)                                                                                                                                       aaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTc             >  5:239274/1‑149 (MQ=255)                                                                                                                                       aaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTc             >  7:323100/1‑149 (MQ=255)                                                                                                                                         aaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCg           <  8:80249/149‑1 (MQ=255)                                                                                                                                          aaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGt          >  5:23417/1‑149 (MQ=255)                                                                                                                                           aTTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGtt         <  2:18983/149‑1 (MQ=255)                                                                                                                                            tttACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTa        <  3:107184/149‑1 (MQ=255)                                                                                                                                            tttACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTa        >  5:84387/1‑149 (MQ=255)                                                                                                                                               aCGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATg      <  3:341149/148‑1 (MQ=255)                                                                                                                                                cGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGt     <  4:13662/148‑1 (MQ=255)                                                                                                                                                cGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGt     <  8:406026/148‑1 (MQ=255)                                                                                                                                                   ttACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGAt                   >  3:156290/1‑131 (MQ=255)                                                                                                                                                   ttACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGAt                   <  4:156290/131‑1 (MQ=255)                                                                                                                                                   ttACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATAATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCACAAAAGTCATGTGATAACAAAGGGTTGAACTATGGCAAGTGGCGATCTTGTCCGTTATGTCAt  >  6:272539/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCAT  >  NC_000913/1807138‑1807431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑