Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,807,103	C→A	P245Q (CCG→CAG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,103	0	C	A	100.0%	109.8 / NA	32	P245Q (CCG→CAG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/16);  total (16/16)

TCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGT  >  NC_000913/1806965‑1807248                                                                                                                                            |                                                                                                                                                  tCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGc                                                                                                                                         >  5:126411/1‑149 (MQ=255)   aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGc                                                                                                                                         >  8:12693/1‑147 (MQ=255)   aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCa                                                                                                                                       <  3:61008/149‑1 (MQ=255)     cccgCCGGACGATGTCCGCAAAGCCGCGCAGGAAATAGTTAATAGCGGCAAGGCCAAACGGGATGTCGTTTCCCT‑CGTACACCAGGAAGGGAGGGTTGTTGATAGTCAAAAAT‑TAATAAAGTGGTTCCACCACATGTGCAAAACCagag                                                                                                                                    >  6:84260/4‑149 (MQ=255)     ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCaga                                                                                                                                     <  7:12693/149‑1 (MQ=255)     ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCa                                                                                                                                       >  5:11352/1‑147 (MQ=255)                     cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTgg                                                                                                                           >  2:104615/1‑143 (MQ=255)                     cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTgg                                                                                                                           <  1:104615/143‑1 (MQ=255)                               cgcAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATg                                                                                                           <  6:11352/149‑1 (MQ=255)                                      aaTCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGgcg                                                                                                    >  6:57791/1‑149 (MQ=255)                                      aaTCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGgcg                                                                                                    >  1:117197/1‑149 (MQ=255)                                          gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                                 <  6:126411/148‑1 (MQ=255)                                             aaTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGTCGCGATGa                                                                                              <  2:117197/148‑1 (MQ=255)                                                         gCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACt                                                                                          <  6:3366/140‑1 (MQ=255)                                                         gCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACt                                                                                          >  5:3366/1‑140 (MQ=255)                                                         gCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                                  >  3:90669/1‑148 (MQ=255)                                                         gCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                                  >  3:98552/1‑148 (MQ=255)                                                           cAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAg                                                                               <  4:90669/149‑1 (MQ=255)                                                                  gTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGc                                                                        <  5:57791/149‑1 (MQ=255)                                                                     gTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCct                                                                      <  4:98552/148‑1 (MQ=255)                                                                         tttCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCtt                                                                 >  2:87267/1‑149 (MQ=255)                                                                                gggTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAaga                                                            <  2:142852/147‑1 (MQ=255)                                                                                gggTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAaga                                                            >  1:142852/1‑147 (MQ=255)                                                                                 ggTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                              >  4:43795/1‑144 (MQ=255)                                                                                 ggTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                              <  3:43795/144‑1 (MQ=255)                                                                                                   gggTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAg                                        <  1:87267/149‑1 (MQ=255)                                                                                                        ttGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                              >  4:144862/1‑122 (MQ=255)                                                                                                        ttGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                              <  3:144862/122‑1 (MQ=255)                                                                                                                             aGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                        >  7:101092/1‑139 (MQ=255)                                                                                                                             aGGTGGTGCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                        <  8:101092/139‑1 (MQ=255)                                                                                                                               gtggtgCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctc                                                                   >  2:54585/1‑94 (MQ=255)                                                                                                                               gtggtgCCACCACAGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctc                                                                   <  1:54585/94‑1 (MQ=255)                                                                                                                                        ccacaGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCa                                                                                <  5:118738/72‑1 (MQ=255)                                                                                                                                        ccacaGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCa                                                                                >  6:118738/1‑72 (MQ=255)                                                                                                                                         cacaGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGt  <  7:4454/149‑1 (MQ=255)                                                                                                                                         cacaGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGt  >  8:4454/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                  TCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGG‑AGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGT  >  NC_000913/1806965‑1807248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑