Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,084,171 C→T 5.0% D581N (GAT→AAT)  speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,084,1710CT 5.0% 324.0 / 4.2 99D581N (GAT→AAT) speAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (48/46);  new base T (3/2);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 107 total aligned reads displayed.

CTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACC  >  NC_000913/3084025‑3084318
                                                                                                                                                  |                                                                                                                                                   
cttcACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATc                                                                                                                                                     <  7:421874/147‑1 (MQ=255)
 ttcACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCCCCATGCCCGCCACGTTATCGCCTTCGTCACACAGTTCTACTTCTACGCTACCGTCAGGGAAGACTACCACGTCAACCTTTTCGGTATCAc                                                                                                                                                   <  8:316802/148‑1 (MQ=255)
   cACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCAcc                                                                                                                                                  >  7:256966/1‑147 (MQ=255)
   cACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCAcc                                                                                                                                                  <  8:141524/147‑1 (MQ=255)
         aTCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAg                                                                                                                                            <  1:238164/147‑1 (MQ=255)
           cgcgGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGtt                                                                                                                                         >  7:162544/1‑148 (MQ=255)
              ggAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTtgtg                                                                                                                                      <  2:429686/148‑1 (MQ=255)
              ggAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTtgtg                                                                                                                                      >  6:324718/1‑148 (MQ=255)
                  cTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAATAGGTTGTGCATg                                                                                                                                  <  7:393681/148‑1 (MQ=255)
                         aaCAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCg                                                                                                                            <  8:256966/147‑1 (MQ=255)
                         aaCAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCg                                                                                                                            <  5:324718/147‑1 (MQ=255)
                            aGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGGTGTGCATGTTGCCGAGGa                                                                                                                        >  7:334853/1‑148 (MQ=255)
                               gTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGAtct                                                                                                                     <  2:196456/148‑1 (MQ=255)
                                 tttCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTcc                                                                                                                   >  1:434537/1‑148 (MQ=255)
                                     ggATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGat                                                                                                               <  6:347493/148‑1 (MQ=255)
                                              tGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGa                                                                                                      >  4:54217/1‑148 (MQ=255)
                                                tACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGa                                                                                                      <  2:312931/146‑1 (MQ=255)
                                                tACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGAc                                                                                                     >  7:169096/1‑147 (MQ=255)
                                                 gcgggttGCAGCATGGCGGCCACGGTGTCGGCGTCGGGAGACAGTTCTACTTCTACGCTAACGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTCTGCATGTTGCCGAGGATCTCCGGATATGCGCCGccc                                                                                                    >  8:112994/6‑147 (MQ=255)
                                                 aCATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCa                                                                                                   >  2:187381/1‑148 (MQ=255)
                                                     atTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGat                                                                                                               <  7:234865/132‑1 (MQ=255)
                                                     atTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGat                                                                                                               >  8:234865/1‑132 (MQ=255)
                                                     atTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATa                                                                                                 >  7:183104/1‑146 (MQ=255)
                                                     atTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATa                                                                                                 >  8:329083/1‑146 (MQ=255)
                                                      ttGCAGCATGTCCGCCACGGTATCGCCATCGACAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACTAACACCTCAAACGCATCGGTATCACCGAACAGCTAGTGCATGTTGCCGAGCATCTCCTGATATGCGCCGACCATa                                                                                                 >  8:363354/1‑145 (MQ=255)
                                                               gTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTtgtg                                                                                                                                      <  3:375079/99‑1 (MQ=255)
                                                               gTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTtgtg                                                                                                                                      >  4:375079/1‑99 (MQ=255)
                                                               gTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGat                                                                                                               <  3:6984/122‑1 (MQ=255)
                                                               gTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGat                                                                                                               >  4:6984/1‑122 (MQ=255)
                                                               gTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAg                                                                                              <  6:334240/139‑1 (MQ=255)
                                                               gTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAg                                                                                              >  5:334240/1‑139 (MQ=255)
                                                               gTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAg                                                                                      >  7:215962/1‑147 (MQ=255)
                                                                 ccGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCAt                                                                                   >  1:78553/1‑148 (MQ=255)
                                                                    ccACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATcgg                                                                                <  2:434537/148‑1 (MQ=255)
                                                                    ccACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATcgg                                                                                <  1:187381/148‑1 (MQ=255)
                                                                          tATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGAtt                                                                          >  2:412117/1‑148 (MQ=255)
                                                                              gCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATtctc                                                                       <  8:169096/147‑1 (MQ=255)
                                                                                cTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTgg                                                                    <  8:416553/148‑1 (MQ=255)
                                                                                  tCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATTACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGAt                                                                  >  1:172249/1‑148 (MQ=255)
                                                                                  tCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGAt                                                                           >  1:180767/1‑139 (MQ=255)
                                                                                   cGTCAGACAGTTCTACTTCTACGCTAGCGTCTGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGa                                                                   <  7:363354/146‑1 (MQ=255)
                                                                                   cGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGa                                                                   <  1:115057/146‑1 (MQ=255)
                                                                                     tCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATTACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               <  2:172249/148‑1 (MQ=255)
                                                                                     tCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATTACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               <  4:164084/148‑1 (MQ=255)
                                                                                     tCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               <  8:215962/148‑1 (MQ=255)
                                                                                     tCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               <  7:112994/148‑1 (MQ=255)
                                                                                      cAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAg                                                                                      <  7:264474/124‑1 (MQ=255)
                                                                                      cAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAg                                                                                      >  8:264474/1‑124 (MQ=255)
                                                                                      cAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               >  3:196642/1‑147 (MQ=255)
                                                                                         aCAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTc                                                           >  4:189439/1‑148 (MQ=255)
                                                                                         aCAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTc                                                           >  1:111828/1‑148 (MQ=255)
                                                                                          cAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCATCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTcc                                                          >  4:176413/1‑148 (MQ=255)
                                                                                             ttctacttctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCggc                                                       <  2:78553/148‑1 (MQ=255)
                                                                                                       acGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAg                                                                                                                                            >  7:81703/1‑53 (MQ=255)
                                                                                                       acGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               >  7:411332/1‑130 (MQ=255)
                                                                                                       acGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               <  8:411332/130‑1 (MQ=255)
                                                                                                        cGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCggcattggcat                                               <  3:189439/145‑1 (MQ=255)
                                                                                                           tACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTctct                                                                      >  5:45330/1‑119 (MQ=255)
                                                                                                           tACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTctct                                                                      <  6:45330/119‑1 (MQ=255)
                                                                                                            aCCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCa                                                                                                   >  3:374596/1‑89 (MQ=255)
                                                                                                            aCCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCa                                                                                                   <  4:374596/89‑1 (MQ=255)
                                                                                                            aCCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTg                                        >  2:289920/1‑148 (MQ=255)
                                                                                                            aCCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACAGAGCATCGGCGGATTCTCTGGATCGTACTACGGCATTGGCATTGTCGTg                                        >  2:257462/1‑148 (MQ=255)
                                                                                                            aCCGTCAGGGAAGAAGAACACGTCAACCGCTTCCGTATCACCGAAAAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGCCCATAAAGAAACCGAGCAACGGCGGATTCACTGGATCGTACTCCGGCATTGGCGTTgtggtg                                        >  8:437715/1‑148 (MQ=255)
                                                                                                             ccGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTg                                        <  8:162544/147‑1 (MQ=255)
                                                                                                               gTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGAt                                                                  <  2:23647/119‑1 (MQ=255)
                                                                                                               gTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGAt                                                                  >  1:23647/1‑119 (MQ=255)
                                                                                                                tCAGGGAAGACGTACACGTCAACTGCTTCGGTATCACCTAACAGGTTGTGCATGTTTCCGAGGATCTCCTTATATGCGCCGACCATAAAGAAACCGAGAATCGGCGGATTCTCTGGATCGt                                                               <  6:111082/121‑1 (MQ=255)
                                                                                                                tCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCaa                                    >  5:110610/1‑148 (MQ=255)
                                                                                                                 cAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACACGTTGTGCATGTTGCCGAGGATCTCCAGATATGCGCCGACCATAAAGAAACCGACCATCGGAGGATTCTCTGGATCGTa                                                              >  5:111082/1‑121 (MQ=255)
                                                                                                                    ggAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATAcc                                >  4:26245/1‑148 (MQ=255)
                                                                                                                     gAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCg                               >  2:232825/1‑148 (MQ=255)
                                                                                                                      aaGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGt                              <  6:110610/148‑1 (MQ=255)
                                                                                                                            aaCACGTCAACCGCTTCTGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATAATCTGGAGCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCAt                        >  4:366555/1‑148 (MQ=255)
                                                                                                                            aaCACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCggc                                                       <  2:46129/117‑1 (MQ=255)
                                                                                                                            aaCACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCggc                                                       >  1:46129/1‑117 (MQ=255)
                                                                                                                            aaCACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCAt                        >  8:280556/1‑148 (MQ=255)
                                                                                                                             acacGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               <  5:271090/108‑1 (MQ=255)
                                                                                                                             acacGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCAt                        >  7:43392/1‑147 (MQ=255)
                                                                                                                             acacGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCAt                        >  7:45383/1‑147 (MQ=255)
                                                                                                                              atgcacAACCTGTTCGGTGATACCGAAGCGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATc                       >  7:43303/6‑147 (MQ=255)
                                                                                                                                cGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAat                    <  7:21609/148‑1 (MQ=255)
                                                                                                                                  tCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGTCCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGGCACCATCAatat                  <  8:334853/148‑1 (MQ=255)
                                                                                                                                  tCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAatat                  <  3:26245/148‑1 (MQ=255)
                                                                                                                                  tCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAatat                  <  8:45383/148‑1 (MQ=255)
                                                                                                                                  tCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAata                   >  5:228057/1‑147 (MQ=255)
                                                                                                                                  tCAACCGCTTCGGTATCACCGAACAGCTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTCGCATTGTCGTGGCAATACCGACACCATCaaaa                   >  2:398168/1‑145 (MQ=255)
                                                                                                                                       cGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGt               <  2:45003/146‑1 (MQ=255)
                                                                                                                                       cGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGt               >  1:45003/1‑146 (MQ=255)
                                                                                                                                       cGCTTCGGTATCACCGAACAGGGTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTgg             <  3:289302/148‑1 (MQ=255)
                                                                                                                                        gCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGt            >  5:159804/1‑148 (MQ=255)
                                                                                                                                        gCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGt            <  1:232825/148‑1 (MQ=255)
                                                                                                                                                 tCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               <  6:306610/88‑1 (MQ=255)
                                                                                                                                                 tCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGt                                                               >  5:306610/1‑88 (MQ=255)
                                                                                                                                                  cACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCg                                          <  7:69133/108‑1 (MQ=255)
                                                                                                                                                  cACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCg                                          >  8:69133/1‑108 (MQ=255)
                                                                                                                                                  cACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTgg                                       >  3:37388/1‑111 (MQ=255)
                                                                                                                                                  cACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATc                       >  2:136044/1‑127 (MQ=255)
                                                                                                                                                  cACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATc                       <  1:136044/127‑1 (MQ=255)
                                                                                                                                                  cACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCAcc  >  4:206081/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTTCACTTGATCGCGGAACTGGGTTAACAGCGTTTTCGGATCGAGCTGTACATATTGCAGCATGTCCGCCACGGTATCGCCTTCGTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACC  >  NC_000913/3084025‑3084318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: