Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,545,924 C→T 5.3% A51V (GCG→GTG)  murQ → N‑acetylmuramic acid 6‑phosphate (MurNAc‑6‑P) etherase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,545,9240CT 5.3% 241.1 / 2.4 75A51V (GCG→GTG) murQN‑acetylmuramic acid 6‑phosphate (MurNAc‑6‑P) etherase
Reads supporting (aligned to +/- strand):  ref base C (36/35);  new base T (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

ATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCGGGTCTGGTGGTTGGTTTGAT  >  NC_000913/2545778‑2546068
                                                                                                                                                  |                                                                                                                                                
aTTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGTATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCgcggc                                                                                                                                                  >  4:47010/1‑147 (MQ=255)
      aaaGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGcgatcgat                                                                                                                                           <  1:191766/148‑1 (MQ=255)
      aaaGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGcgatcga                                                                                                                                            <  3:225333/147‑1 (MQ=255)
        aGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGTATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGt                                                                                                                                         <  3:47010/148‑1 (MQ=255)
         gatgatTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGt                                                                                                                                         >  8:236402/1‑147 (MQ=255)
             atTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATcc                                                                                                                                    <  1:183865/148‑1 (MQ=255)
                   gAAGGCTCGAACACCGCCTCGTCTGAAATTGACCGAGTATCGACGCTTGATATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGACGTTTAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGcc                                                                                                                               >  2:34324/1‑147 (MQ=255)
                    aaGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGcc                                                                                                                               >  3:317516/1‑146 (MQ=255)
                    aaGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCa                                                                                                                             <  7:403994/148‑1 (MQ=255)
                       gCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGAAGTTATcc                                                                                                                                    >  7:145347/1‑138 (MQ=255)
                       gCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGAAGTTATcc                                                                                                                                    <  8:145347/138‑1 (MQ=255)
                         tCGAACACCGCCTCGGCTGTTATTGACCGCGTATCGACGCTGGATATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGGGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCa                                                                                                                             <  1:324611/143‑1 (MQ=255)
                          cGAACACCGCCTCGTCTGAAAATGACCGCGTATCGACTCTGTAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGACAGCGTACTGCCGCATATCGCCGCGGCGATCGAAGATATCCACGCCCAgcagaa                                                                                                                       >  2:324611/1‑143 (MQ=255)
                            aaCACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATg                                                                                                                                          <  8:174268/127‑1 (MQ=255)
                            aaCACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATg                                                                                                                                          >  7:174268/1‑127 (MQ=255)
                            aaCACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAgcg                                                                                                                     >  5:138313/1‑148 (MQ=255)
                            aaCACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCAGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAgcg                                                                                                                     >  7:298946/1‑148 (MQ=255)
                               acCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATcc                                                                                                                                    <  2:221294/130‑1 (MQ=255)
                               acCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATcc                                                                                                                                    >  1:221294/1‑130 (MQ=255)
                               acCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAgcggcg                                                                                                                  <  6:231857/148‑1 (MQ=255)
                               acCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAgcggcg                                                                                                                  <  4:317516/148‑1 (MQ=255)
                                    cTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCggcggg                                                                                                             >  3:188680/1‑148 (MQ=255)
                                      cGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCggcgggcg                                                                                                           >  4:187343/1‑148 (MQ=255)
                                         cTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCt                                                                                                        >  3:389846/1‑148 (MQ=255)
                                         cTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCt                                                                                                        <  4:389846/148‑1 (MQ=255)
                                          tGAAATTGACCGAGTATCGACGCTGTAAATGTGCCGTATTATCAACGATGAAGATAAAACCGTACCGCTTGCAGTTTAGAGCGTAGTGCCGTATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGGGTCTg                                                                                                       <  8:211953/148‑1 (MQ=255)
                                            aaaTTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTg                                                                                                       <  7:236402/146‑1 (MQ=255)
                                            aaaTTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGa                                                                                                      <  6:116456/147‑1 (MQ=255)
                                            aaaTTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGAt                                                                                                     >  6:303085/1‑148 (MQ=255)
                                             aaTTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGAtt                                                                                                    <  8:436424/148‑1 (MQ=255)
                                             aaTTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGAtt                                                                                                    >  7:436424/1‑148 (MQ=255)
                                                  aCCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCt                                                                                               >  2:380989/1‑148 (MQ=255)
                                                    cgcgTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCCGACCGCTTGCCGTTGAGCGCGTACTGCCGTATATCGCCGCGTCTATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCg                                                                                             <  1:34324/148‑1 (MQ=255)
                                                      cgTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCt                                                                                                        >  3:179799/1‑135 (MQ=255)
                                                      cgTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCt                                                                                                        <  4:179799/135‑1 (MQ=255)
                                                         aTCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCg                                                                                        >  3:340364/1‑148 (MQ=255)
                                                          tCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCg                                                                                        <  4:188680/147‑1 (MQ=255)
                                                          tCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCg                                                                                        <  3:348797/147‑1 (MQ=255)
                                                           cGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGcgatcgat                                                                                                                                           <  6:320945/95‑1 (MQ=255)
                                                           cGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGcgatcgat                                                                                                                                           >  5:320945/1‑95 (MQ=255)
                                                              cGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCATTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGaa                                                                                    >  1:292690/1‑147 (MQ=255)
                                                                  ggAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATcc                                                                               >  6:36921/1‑148 (MQ=255)
                                                                   gAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATc                                                                                <  2:160259/146‑1 (MQ=255)
                                                                    aaaTGTGCCGGATTGTCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCgg                                                                             >  4:413482/1‑148 (MQ=255)
                                                                         tgCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGgtcgtc                                                                        <  4:340364/148‑1 (MQ=255)
                                                                           ccGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTg                                                                      >  8:69966/1‑148 (MQ=255)
                                                                            cGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTgg                                                                     <  3:187343/148‑1 (MQ=255)
                                                                            cGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTgg                                                                     <  7:376275/148‑1 (MQ=255)
                                                                            cGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTgg                                                                     <  1:380989/148‑1 (MQ=255)
                                                                                  aTCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTc                                                               >  2:322689/1‑148 (MQ=255)
                                                                                   tCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCt                                                              >  7:352862/1‑148 (MQ=255)
                                                                                   tCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCt                                                              <  1:322689/148‑1 (MQ=255)
                                                                                   tCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCt                                                              <  5:36921/148‑1 (MQ=255)
                                                                                     aaCGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTgg                                                            <  3:413482/148‑1 (MQ=255)
                                                                                     aaCGATGAAGATAAAACCGTACCGCTTGCCGGTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTgg                                                            <  5:303085/148‑1 (MQ=255)
                                                                                      aCGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGa                                                           >  5:12115/1‑148 (MQ=255)
                                                                                      aCGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGa                                                           <  2:247219/148‑1 (MQ=255)
                                                                                          tGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGAGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGcc                                                       <  6:138313/148‑1 (MQ=255)
                                                                                           gAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGc                                                        >  7:394474/1‑146 (MQ=255)
                                                                                              gATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGc                                                    >  7:51703/1‑147 (MQ=255)
                                                                                               aTAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCg                                                   >  6:219629/1‑147 (MQ=255)
                                                                                                tAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGaa                                                 >  2:43503/1‑148 (MQ=255)
                                                                                                    aCCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTc                                             >  1:100132/1‑148 (MQ=255)
                                                                                                       gTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGc                                          <  1:231329/148‑1 (MQ=255)
                                                                                                          ccGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCa                                       >  2:82253/1‑148 (MQ=255)
                                                                                                                  cGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGc                               >  7:415028/1‑148 (MQ=255)
                                                                                                                      gAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTg                            <  8:51703/147‑1 (MQ=255)
                                                                                                                        gcgcGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGaaa                         >  1:303970/1‑148 (MQ=255)
                                                                                                                         cgcgTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAAc                        >  5:154609/1‑148 (MQ=255)
                                                                                                                           cgTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCg                      <  8:394474/148‑1 (MQ=255)
                                                                                                                                 gCCGGATATCGCCGCGGTGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGAtt                                                                >  4:371036/1‑100 (MQ=255)
                                                                                                                                 gCCGGATATCGCCGCGGTGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGAtt                                                                <  3:377016/100‑1 (MQ=255)
                                                                                                                                 gCCGGATATCGCCGCGGTGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGAtt                                                                >  4:377016/1‑100 (MQ=255)
                                                                                                                                 gCCGGATATCGCCGCGGTGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGAtt                                                                <  3:371036/100‑1 (MQ=255)
                                                                                                                                 gCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCGGGTCtg                >  7:44724/1‑148 (MQ=255)
                                                                                                                                   cGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCGGGTCtggt              >  1:266629/1‑148 (MQ=255)
                                                                                                                                         tCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGaa                                                 <  6:414000/107‑1 (MQ=255)
                                                                                                                                         tCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCGGGTCTGGtggttg         <  7:69966/147‑1 (MQ=255)
                                                                                                                                         tCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACATCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGaa                                                 >  5:414000/1‑107 (MQ=255)
                                                                                                                                           gccgcGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCGGGTCTGGtggttggt       <  8:415028/147‑1 (MQ=255)
                                                                                                                                               cggcgATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCGGGTCTGGTGGTTGGTTTGAt  <  6:12115/148‑1 (MQ=255)
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ATTTGAAAAGATGATTACTGAAGGCTCGAACACCGCCTCGGCTGAAATTGACCGCGTATCGACGCTGGAAATGTGCCGGATTATCAACGATGAAGATAAAACCGTACCGCTTGCCGTTGAGCGCGTACTGCCGGATATCGCCGCGGCGATCGATGTTATCCACGCCCAGGTAAGCGGCGGCGGGCGTCTGATTTACCTCGGTGCGGGAACATCCGGTCGTCTGGGGATTCTGGATGCCAGCGAATGTCCGCCCACCTACGGCGTGAAACCGGGTCTGGTGGTTGGTTTGAT  >  NC_000913/2545778‑2546068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: