Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,219,633 C→A 5.3% intergenic (‑152/+59) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,219,6330CA 5.3% 328.1 / 5.5 95intergenic (‑152/+59)osmF/bglXputative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (40/50);  new base A (2/3);  total (42/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

TTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTT  >  NC_000913/2219487‑2219778
                                                                                                                                                  |                                                                                                                                                 
tttCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATgcg                                                                                                                                                  >  1:164490/1‑148 (MQ=255)
      cATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGgcgc                                                                                                                                              >  2:212889/1‑146 (MQ=255)
      cATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGgcgc                                                                                                                                              >  7:271562/1‑146 (MQ=255)
       aTTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGGTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAg                                                                                                                                           <  6:272395/148‑1 (MQ=255)
        ttCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAgc                                                                                                                                          >  8:454432/1‑148 (MQ=255)
        ttCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAgc                                                                                                                                          >  3:67666/1‑148 (MQ=255)
         tCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAgcg                                                                                                                                         >  2:297861/1‑148 (MQ=255)
         tCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAgcg                                                                                                                                         >  1:83957/1‑148 (MQ=255)
             ttACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAgcgc                                                                                                                                        <  2:316690/145‑1 (MQ=255)
               aCTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTa                                                                                                                                    <  2:5378/147‑1 (MQ=255)
                cTGTTGTTTTCAGCGAATTAAGATAAAAGCATAGTTGATAATGGCGGGGTTAGGTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                  <  2:175741/148‑1 (MQ=255)
                cTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                  <  8:51960/148‑1 (MQ=255)
                  gttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                                >  8:293817/1‑148 (MQ=255)
                  gttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                                >  3:134843/1‑148 (MQ=255)
                   ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                               >  7:455720/1‑148 (MQ=255)
                   ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                               <  2:273913/148‑1 (MQ=255)
                   ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGCCGCATcc                                                                                                                                 <  1:319818/146‑1 (MQ=255)
                   ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGCCGCATcc                                                                                                                                 >  2:319818/1‑146 (MQ=255)
                    tgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                               <  3:11383/147‑1 (MQ=255)
                    tgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                               >  4:11383/1‑147 (MQ=255)
                     gttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                              <  2:164490/147‑1 (MQ=255)
                     gttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGCCGCATCCGGc                                                                                                                              <  6:429098/147‑1 (MQ=255)
                           aGCGAATTAAGTGAAAAGCTTAGTTGATATTGGCTGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCATATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACaa                                                                                                                        <  8:305990/147‑1 (MQ=39)
                           aGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACaa                                                                                                                        <  3:398390/147‑1 (MQ=255)
                           aGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCGTATCCGGCCTACaa                                                                                                                        <  1:297861/147‑1 (MQ=255)
                            gCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGcc                                                                                                                                       >  2:338431/1‑131 (MQ=255)
                            gCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGcc                                                                                                                                       <  1:338431/131‑1 (MQ=255)
                              gAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAggg                                                                                                                    >  8:366597/1‑148 (MQ=255)
                              gAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAggg                                                                                                                    <  1:212889/148‑1 (MQ=255)
                              gAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAggg                                                                                                                    <  3:226803/148‑1 (MQ=255)
                               aaTTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGgc                                                                                                                   <  3:5405/148‑1 (MQ=255)
                                aTTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGgcg                                                                                                                  <  7:293817/148‑1 (MQ=255)
                                   aaGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCa                                                                                                                >  8:456132/1‑147 (MQ=255)
                                    agagAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCaa                                                                                                               <  4:348019/147‑1 (MQ=255)
                                     gagaAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAAc                                                                                                             >  7:448935/1‑148 (MQ=255)
                                           aGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTcgcg                                                                                                       <  8:448935/148‑1 (MQ=255)
                                                aGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACa                                                                                                                         >  4:244658/1‑125 (MQ=255)
                                                aGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACa                                                                                                                         <  3:244658/125‑1 (MQ=255)
                                                aGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCt                                                                                                   >  4:236938/1‑147 (MQ=255)
                                                 gTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTa                                                                                                 >  6:282713/1‑148 (MQ=255)
                                                 gTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTa                                                                                                 >  6:99267/1‑148 (MQ=255)
                                                   tGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTAc                                                                                                >  3:188474/1‑147 (MQ=255)
                                                    gATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACt                                                                                               <  8:271562/147‑1 (MQ=255)
                                                     atcaTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTaa                                                                                             <  3:23370/145‑1 (MQ=255)
                                                     aTAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTaa                                                                                             <  7:454432/148‑1 (MQ=255)
                                                     aTAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTa                                                                                              <  7:456132/147‑1 (MQ=255)
                                                       aaTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTTAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAg                                                                                           >  1:384746/1‑148 (MQ=255)
                                                        aTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAg                                                                                           >  7:225555/1‑147 (MQ=255)
                                                        aTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAg                                                                                           <  4:188474/147‑1 (MQ=255)
                                                        aTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGc                                                                                          <  3:236938/148‑1 (MQ=255)
                                                          ggCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCAt                                                                                        >  7:328225/1‑148 (MQ=255)
                                                             ggggTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTa                                                                                      >  5:350886/1‑147 (MQ=255)
                                                              gggTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCACTTACTAAAGCATTa                                                                                      >  4:92335/1‑146 (MQ=255)
                                                                  tAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                            >  1:84723/1‑104 (MQ=255)
                                                                  tAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                            <  2:84723/104‑1 (MQ=255)
                                                                  tAGCTTGAGCCGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCaa                                                                                >  4:402660/1‑148 (MQ=255)
                                                                    gCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATTAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACt                                                                              <  3:129879/148‑1 (MQ=255)
                                                                     cTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTc                                                                             <  4:67666/148‑1 (MQ=255)
                                                                      ttGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTc                                                                             >  1:429865/1‑147 (MQ=255)
                                                                       tGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCa                                                                            >  2:184564/1‑147 (MQ=255)
                                                                       tGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCa                                                                            >  1:180525/1‑147 (MQ=255)
                                                                                ggATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCtt                                                                  <  7:366597/148‑1 (MQ=255)
                                                                                ggATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCtt                                                                  <  8:78321/148‑1 (MQ=255)
                                                                                 gATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCttt                                                                 <  8:341207/148‑1 (MQ=255)
                                                                                 gATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCttt                                                                 <  8:328225/148‑1 (MQ=255)
                                                                                     cAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCtt                                                              >  1:106622/1‑147 (MQ=255)
                                                                                      aGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTaa                                                            <  8:455720/148‑1 (MQ=255)
                                                                                      aGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTa                                                             <  5:99267/147‑1 (MQ=255)
                                                                                       gTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTAc                                                                                     <  1:160199/122‑1 (MQ=255)
                                                                                       gTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTAc                                                                                     >  2:160199/1‑122 (MQ=255)
                                                                                        ttGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCTCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAAcg                                                          <  4:91017/148‑1 (MQ=255)
                                                                                               aTCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGAGTgag                                                    >  3:89142/1‑145 (MQ=255)
                                                                                                   gATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATc                                               <  2:348461/148‑1 (MQ=255)
                                                                                                   gATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATc                                               <  2:83957/148‑1 (MQ=255)
                                                                                                    aTAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                              <  2:429865/148‑1 (MQ=255)
                                                                                                    aTAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                              <  3:402660/148‑1 (MQ=255)
                                                                                                        aTACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                              <  2:328703/144‑1 (MQ=255)
                                                                                                        aTACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                              >  1:328703/1‑144 (MQ=255)
                                                                                                          aCTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGcc                                         >  7:387868/1‑147 (MQ=255)
                                                                                                             tAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGAt                                      >  8:409550/1‑147 (MQ=255)
                                                                                                               agagGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATaa                                    <  8:225555/147‑1 (MQ=255)
                                                                                                                   gCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                            >  2:315083/1‑55 (MQ=255)
                                                                                                                   gCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                            <  1:315083/55‑1 (MQ=255)
                                                                                                                       atTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTg                            >  2:391559/1‑147 (MQ=255)
                                                                                                                       atTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTg                            >  5:269387/1‑147 (MQ=255)
                                                                                                                        ttCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGaa                          <  1:66670/148‑1 (MQ=255)
                                                                                                                        ttCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGa                           <  1:391559/147‑1 (MQ=255)
                                                                                                                        ttCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGa                           <  6:269387/147‑1 (MQ=255)
                                                                                                                             tCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                         >  3:214790/1‑144 (MQ=255)
                                                                                                                             tCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                         <  4:214790/144‑1 (MQ=255)
                                                                                                                               cGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGGCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGcc                    <  4:134843/147‑1 (MQ=255)
                                                                                                                                ggCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCtt                                                                                                  >  6:130387/1‑68 (MQ=255)
                                                                                                                                ggCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCtt                                                                                                  <  5:130387/68‑1 (MQ=255)
                                                                                                                                        aggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                         >  8:260575/3‑133 (MQ=39)
                                                                                                                                        aggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                         <  7:260575/131‑1 (MQ=39)
                                                                                                                                           ccGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCACTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTc        <  3:92335/147‑1 (MQ=255)
                                                                                                                                                 gcggcgCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATAttt  >  5:281838/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTT  >  NC_000913/2219487‑2219778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: