Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,025,992 T→A 6.0% F161Y (TTT→TAT)  yedP → putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,9920TA 6.0% 222.0 / 3.1 67F161Y (TTT→TAT) yedPputative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (29/34);  new base A (2/2);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

ATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAAC  >  NC_000913/2025845‑2026133
                                                                                                                                                   |                                                                                                                                             
aTTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAAtt                                                                                                                                               <  1:38399/148‑1 (MQ=255)
 ttttAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAAttt                                                                                                                                              <  3:160094/148‑1 (MQ=255)
  tttAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTa                                                                                                                                             >  1:229062/1‑148 (MQ=255)
   ttAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTa                                                                                                                                             <  1:126992/147‑1 (MQ=255)
                ttttGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTgaac                                                                                                                               >  5:122581/1‑148 (MQ=255)
                   tgatgaTGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTgaacga                                                                                                                             <  5:83853/147‑1 (MQ=255)
                   tgatgaTGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTgaacga                                                                                                                             <  7:442636/147‑1 (MQ=255)
                        atgTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTggg                                                                                                                       >  4:327845/1‑148 (MQ=255)
                              acgaTGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTa                                                                                                                   >  7:187270/1‑146 (MQ=255)
                                 aTGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGtt                                                                                                              <  8:343173/148‑1 (MQ=255)
                                 aTGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGtt                                                                                                              <  5:86972/148‑1 (MQ=255)
                                      aCCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGc                                                                                                         <  1:427887/148‑1 (MQ=255)
                                      aCCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATATACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGc                                                                                                         >  2:9487/1‑148 (MQ=255)
                                      aCCATCGACGAATGGACGGGATTAAGCCGTAGCAAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATATACCGCTAGTCTGAACGAACTGGGCTTACAGTTTATGc                                                                                                         >  2:2573/1‑148 (MQ=255)
                                        cATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTga                                                                                                                                 <  1:183122/122‑1 (MQ=255)
                                        cATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTga                                                                                                                                 >  2:183122/1‑122 (MQ=255)
                                        cATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAg                                                                                                       <  2:229062/148‑1 (MQ=255)
                                         aTCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTg                                                                                                                         >  6:364339/1‑129 (MQ=255)
                                         aTCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTg                                                                                                                         <  5:364339/129‑1 (MQ=255)
                                           cGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTgaacga                                                                                                                             >  5:157343/1‑123 (MQ=255)
                                           cGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTgaacga                                                                                                                             <  6:157343/123‑1 (MQ=255)
                                              cGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTgcg                                                                                                 >  6:70565/1‑148 (MQ=255)
                                              cGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTgcg                                                                                                 >  2:139241/1‑148 (MQ=255)
                                                  tGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCt                                                                                             <  3:327845/148‑1 (MQ=255)
                                                      cGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTg                                                                                         >  5:237838/1‑148 (MQ=255)
                                                            tAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTgaacgaa                                                                                                                            >  4:204452/1‑107 (MQ=255)
                                                            tAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTgaacgaa                                                                                                                            <  3:204452/107‑1 (MQ=255)
                                                             aaGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTa                                                                                  >  4:376164/1‑148 (MQ=255)
                                                                ccGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTg                                                                               <  7:232902/148‑1 (MQ=255)
                                                                ccGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTg                                                                               <  8:125965/148‑1 (MQ=255)
                                                                   tAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACt                                                                                >  8:138964/1‑144 (MQ=255)
                                                                   tAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACt                                                                                <  7:138964/144‑1 (MQ=255)
                                                                                   aCGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTAcc                                                                                                                                           <  6:238119/69‑1 (MQ=255)
                                                                                   aCGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTAca                                                                                                                                           >  5:238119/1‑68 (MQ=255)
                                                                                   aCGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGaaa                                                              <  1:139241/146‑1 (MQ=255)
                                                                                    cGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGa                                                           <  1:236059/148‑1 (MQ=255)
                                                                                     gCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGAt                                                          <  2:404062/148‑1 (MQ=255)
                                                                                           tCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGt                                                                                   >  4:127966/1‑117 (MQ=255)
                                                                                           tCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGt                                                                                   <  3:127966/117‑1 (MQ=255)
                                                                                            cATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGc                                                                                       >  7:30040/1‑112 (MQ=255)
                                                                                            cATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGc                                                                                       <  8:30040/112‑1 (MQ=255)
                                                                                             aTGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCa                                                                                      <  2:31801/112‑1 (MQ=255)
                                                                                             aTGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCa                                                                                      >  1:31801/1‑112 (MQ=255)
                                                                                             aTGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATATACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCGGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTg                                                   <  1:9487/147‑1 (MQ=255)
                                                                                             aTGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATATACCGCTCGTCTGAACGAACTGGGATTACAGTTTATGCGGGTTGCTCGATTCTGGCAAGTACTGTATGCTTCTGCCGGAAAAGATCAGTCTg                                                   <  1:2573/147‑1 (MQ=255)
                                                                                               gAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGcc                                                 >  1:82618/1‑147 (MQ=255)
                                                                                                aGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCa                                                <  8:187270/147‑1 (MQ=255)
                                                                                                      cGGTAACGCTAATCTGGCGCTACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGttt                                                                                                             <  4:227937/80‑1 (MQ=255)
                                                                                                      cGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTggg                                                                                                                       <  3:200614/70‑1 (MQ=255)
                                                                                                      cGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTggg                                                                                                                       >  4:200614/1‑70 (MQ=255)
                                                                                                      cGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGttt                                                                                                             >  3:227937/1‑80 (MQ=255)
                                                                                                      cGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGAt                                         >  7:455240/1‑148 (MQ=255)
                                                                                                        gTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTa                                       >  2:135385/1‑148 (MQ=255)
                                                                                                              cTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGaaa                                                              >  5:423245/1‑119 (MQ=255)
                                                                                                              cTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGaaa                                                              <  6:423245/119‑1 (MQ=255)
                                                                                                                   cTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAgg                                                                                                      <  8:60281/74‑1 (MQ=255)
                                                                                                                   cTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAgg                                                                                                      >  7:60281/1‑74 (MQ=255)
                                                                                                                      gcgcGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTAt                            <  2:25448/145‑1 (MQ=255)
                                                                                                                      gcgcGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTAt                            <  8:286271/145‑1 (MQ=255)
                                                                                                                        gcgACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATcaaca                       <  8:455240/148‑1 (MQ=255)
                                                                                                                           aCAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAAtt                    >  5:20741/1‑148 (MQ=255)
                                                                                                                               tGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATc                                                         >  7:238619/1‑107 (MQ=255)
                                                                                                                               tGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATc                                                         <  8:238619/107‑1 (MQ=255)
                                                                                                                               tGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCa                <  5:70565/148‑1 (MQ=255)
                                                                                                                                gACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAg               >  1:337068/1‑148 (MQ=255)
                                                                                                                                       gTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACg        <  6:20741/148‑1 (MQ=255)
                                                                                                                                              acaATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTc                                                                       <  3:53748/78‑1 (MQ=255)
                                                                                                                                              acaATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTc                                                                       >  4:53748/1‑78 (MQ=255)
                                                                                                                                              acaATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAAc  >  8:424442/1‑147 (MQ=255)
                                                                                                                                                  tttACCGCTCGTCTGAACGATCTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTa                                       >  1:511/1‑106 (MQ=255)
                                                                                                                                                  tttACCGCTCGTCTGAACGATCTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTa                                       <  2:511/106‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
ATTTTAAATTCACGACTTTTGATGATGTCGACGATGCAACCATCGCCGAATGGACGGGATTAAGCCGTAGCCAGGCGGCGCTGACGCAGCTTCATGAGGCGTCGGTAACGCTAATCTGGCGCGACAGTGACGAGCGTATGGCACAATTTACCGCTCGTCTGAACGAACTGGGCTTACAGTTTATGCAGGGTGCGCGCTTCTGGCACGTACTGGATGCTTCTGCCGGAAAAGATCAGGCTGCCAACTGGATTATCGCGACCTATCAACAATTGTCAGGCAAACGCCCAAC  >  NC_000913/2025845‑2026133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: