Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,237,971 A→G 5.5% Q134R (CAG→CGG)  dadA → D‑amino acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,237,9710AG 5.5% 222.9 / 3.0 72Q134R (CAG→CGG) dadAD‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (33/35);  new base G (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

AGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGT  >  NC_000913/1237824‑1238115
                                                                                                                                                   |                                                                                                                                                
aGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCa                                                                                                                                                  <  4:173075/148‑1 (MQ=255)
   tGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAggg                                                                                                                                               >  8:107035/1‑148 (MQ=255)
    gAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAggg                                                                                                                                               <  5:29591/147‑1 (MQ=255)
     acaTGGATGTGGCAAATGGTACGTAACTGCGACACCATCCACGAGATGGCACAGAACTGGCGGTTTGTGCGTCTGGCGGTATACAGCCGTGCTGGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGt                                                                                                                                              <  1:321569/145‑1 (MQ=255)
         ggATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGAc                                                                                                                                         >  1:110530/1‑148 (MQ=255)
           aTGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAgg                                                                                                                                                >  4:9162/1‑139 (MQ=255)
           aTGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAgg                                                                                                                                                <  3:9162/139‑1 (MQ=255)
                gCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCa                                                                                                                                   <  6:162906/147‑1 (MQ=255)
                gCAAATGTTACGTAACTGCGACACCAGCAACTACATGGAAAAAAAAGGGCTGATTGTGCGTCTGGAGGAATACAGCCTGGATTGCCTGAAAGCATTGCGAGCCGATCCGAATATTAATTATGAAGTCATTCAGGTTGTGACGCTTCaa                                                                                                                                  >  8:315217/1‑148 (MQ=255)
                 cAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAAc                                                                                                                                 >  6:313052/1‑148 (MQ=255)
                 cAAATGTTACGTAACTGCGACAAAATCCACTACATGGAAAACAAAGGTAGTCTGGTGAGTCTGGCGAAATAAAGCCGTGATTGCCATAAAGCATTGCGAGCAGAAACCAATATAAAGAATTAAGGGCGTCACAGTCGGACCCAGCaa                                                                                                                                  >  4:334336/1‑147 (MQ=255)
                    aTGTTACCTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGACTACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGt                                                                                                                              <  5:393250/148‑1 (MQ=255)
                     tGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGtt                                                                                                                             <  3:181343/148‑1 (MQ=255)
                     tGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGtt                                                                                                                             >  6:185108/1‑148 (MQ=255)
                     tGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGtt                                                                                                                             <  3:106545/148‑1 (MQ=255)
                       ttACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTc                                                                                                                            <  2:110530/147‑1 (MQ=255)
                       ttACGTAACTGCGAAACCAGACACTACATGGAAAACAAATGGAGGATGGTGCGTCTGGCTGTCTACAGACGTGATTGCCTGAAAGCATTGAGATACGAAACAAATATTCATTATGAATGTAGTCAGGGTGGGACGCTGCAACTGATc                                                                                                                            <  7:315217/147‑1 (MQ=255)
                          cGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCAGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTa                                                                                                                        <  3:169261/148‑1 (MQ=255)
                                  cGACATCAGCCACTACATGGAAAACAAAGGTCGGAGGGTGCGTCTGGCGGAATACAGCAGTGATTGCCTGAAAGCATTGCGCGCCGAAAGCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGc                                                                                                                                       <  6:319783/125‑1 (MQ=255)
                                  cGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGaaca                                                                                                                 <  7:107035/147‑1 (MQ=255)
                                   gACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGTCGTAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCATCGTGGGACGCt                                                                                                                                      >  5:319783/1‑125 (MQ=255)
                                         aGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGt                                                                                                                         >  8:28776/1‑132 (MQ=255)
                                         aGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGt                                                                                                                         <  7:28776/132‑1 (MQ=255)
                                         aGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTAt                                                                                                          >  2:119395/1‑147 (MQ=255)
                                            cACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGaaa                                                                                                      <  8:195928/148‑1 (MQ=255)
                                               tACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATg                                                                                                   >  2:383109/1‑148 (MQ=255)
                                                aCATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGc                                                                                                  >  4:306673/1‑148 (MQ=255)
                                                 cATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCg                                                                                                 <  3:306673/148‑1 (MQ=255)
                                                    ggAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAg                                                                                                             >  6:303701/1‑133 (MQ=255)
                                                    ggAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAg                                                                                                             <  5:303701/133‑1 (MQ=255)
                                                            aaGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTGCCGTACCGAACAACAGTATGAAAATGCGCCCCGCGatat                                                                                      >  6:35799/1‑148 (MQ=255)
                                                              gggCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGaacaac                                                                                                               >  5:7977/1‑121 (MQ=255)
                                                              gggCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGaacaac                                                                                                               <  6:7977/121‑1 (MQ=255)
                                                                 cGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAt                                                                                                    >  2:320408/1‑129 (MQ=255)
                                                                 cGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAAt                                                                                                    <  1:320408/129‑1 (MQ=255)
                                                                     tGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                                                                             <  5:313052/148‑1 (MQ=255)
                                                                      ggTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                                                                             <  3:334336/147‑1 (MQ=255)
                                                                        tGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTgg                                                                           >  7:187610/1‑147 (MQ=255)
                                                                         gCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGaa                                                                                                                   <  1:109230/106‑1 (MQ=255)
                                                                         gCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGaa                                                                                                                   >  2:109230/1‑106 (MQ=255)
                                                                          cGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAg                                                                        <  8:187610/148‑1 (MQ=255)
                                                                           gTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGa                                                                       <  1:128716/148‑1 (MQ=255)
                                                                               ggCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGcc                                                                   >  2:57194/1‑148 (MQ=255)
                                                                                gCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGATAACAATATTCAGTATGAATGGCGTCAggg                                                                                                                                               >  5:295615/1‑71 (MQ=255)
                                                                                            cGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGCCCCGCGATAGCGCCGTGCTGGAAGATGCCGGCGTACCGTATc                                                      >  5:102182/1‑148 (MQ=255)
                                                                                              tGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAg                                                    <  5:185108/148‑1 (MQ=255)
                                                                                                        aaaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAg                                                                        <  7:246890/118‑1 (MQ=255)
                                                                                                        aaaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAg                                                                        >  8:246890/1‑118 (MQ=255)
                                                                                                        aaaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGaa                                           <  1:57194/147‑1 (MQ=255)
                                                                                                        aaaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAAt                                          <  1:383109/148‑1 (MQ=255)
                                                                                                         aaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAg                                                                                                             <  5:250045/80‑1 (MQ=255)
                                                                                                         aaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAg                                                                                                             >  6:250045/1‑80 (MQ=255)
                                                                                                         aaGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCCGGAATc                                         >  1:302963/1‑148 (MQ=255)
                                                                                                               tGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCGGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTCCTGGAATCCAgccg                                   >  7:197716/1‑148 (MQ=255)
                                                                                                                   cgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                                                                             <  6:123181/102‑1 (MQ=255)
                                                                                                                   cgcCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                                                                             >  5:123181/1‑102 (MQ=255)
                                                                                                                      cGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCCGGAATCCAGCCGCCTGGCg                            <  2:302963/148‑1 (MQ=255)
                                                                                                                               tatTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGa                          >  8:360054/1‑141 (MQ=255)
                                                                                                                               tatTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGa                          <  7:360054/141‑1 (MQ=255)
                                                                                                                                atTCAGTATGAAGGGCGTCGGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCTTGCTGGAAg                                                                        >  2:175714/1‑94 (MQ=255)
                                                                                                                                atTCAGTATGAAGGGCGTCGGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCTTGCTGGAAg                                                                        <  1:175714/94‑1 (MQ=255)
                                                                                                                                atTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAg                                                                        <  4:264020/94‑1 (MQ=255)
                                                                                                                                atTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAg                                                                        >  3:264020/1‑94 (MQ=255)
                                                                                                                                      tATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACGACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                                                                             <  4:108568/83‑1 (MQ=255)
                                                                                                                                      tATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACGACAGTATGAAAATGCGACCCGCGATATCGCCGTGCt                                                                             >  3:108568/1‑83 (MQ=255)
                                                                                                                                         gAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGat                                                                                        >  8:447585/1‑69 (MQ=255)
                                                                                                                                         gAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGat                                                                                        <  7:447585/69‑1 (MQ=255)
                                                                                                                                         gAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTgg         >  8:330523/1‑148 (MQ=255)
                                                                                                                                         gAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGACGGAAGTGGAGCCCGCGCTgg         >  6:27388/1‑148 (MQ=255)
                                                                                                                                               cGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAg   >  6:393688/1‑148 (MQ=255)
                                                                                                                                                gTCGGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGt  <  8:197716/148‑1 (MQ=255)
                                                                                                                                                gTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCcgcg             <  8:218678/137‑1 (MQ=255)
                                                                                                                                                gTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCcgcg             >  7:218678/1‑137 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGT  >  NC_000913/1237824‑1238115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: