Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 755,400 | G→A | 100% | W75* (TGG→TAG) | sdhC → | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 755,400 | 0 | G | A | 98.7% | 252.1 / ‑4.3 | 74 | W75* (TGG→TAG) | sdhC | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (41/32); total (42/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTT > NC_000913/755260‑755542 | tctcCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATc > 8:148245/1‑148 (MQ=255) ctcCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATc < 7:148245/147‑1 (MQ=255) ccGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTgg < 4:224142/147‑1 (MQ=255) gATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAc > 7:435735/1‑148 (MQ=255) cTTTGTTGCAGTGGGCATCCTGCTGTGGCTACTGGGTACCAGCCTCTCTTCCCCGGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGACAAATTTATCATGTAGGGCCTCCTTACCGCTCTGGCGTATCAcgtcgtc > 4:52312/1‑148 (MQ=255) gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcgca < 1:7647/147‑3 (MQ=255) gCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGt < 8:435735/147‑1 (MQ=255) tGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTTGCGTATCACGTCGTCGTAGGTATTCg > 1:262600/1‑148 (MQ=255) ggCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGc > 6:309715/1‑147 (MQ=255) gCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCGTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCca < 5:309715/148‑1 (MQ=255) ccTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACat > 5:93325/1‑147 (MQ=255) ctgTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatg < 1:294990/147‑1 (MQ=255) gtgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcg > 8:109865/1‑119 (MQ=255) gtgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCAcg < 7:109865/119‑1 (MQ=255) tgGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgatg > 7:337231/1‑147 (MQ=255) tCTGGGTAACAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATATATCATGTAGGTCATCCTTAAAGCTCTGGCGTATCACGTCGTCGTAGGTATACGCCACATGAAGATCGAtttt > 1:178705/1‑148 (MQ=255) tGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTgg > 5:112118/1‑148 (MQ=255) gggTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCcaca < 5:301601/131‑1 (MQ=255) gggTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCcaaa > 6:301601/1‑129 (MQ=255) ggTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCt < 1:59034/148‑1 (MQ=255) gTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTa > 6:387657/1‑90 (MQ=255) gTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTa < 5:387657/90‑1 (MQ=255) gTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCg > 4:58382/1‑101 (MQ=255) gTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCg < 3:58382/101‑1 (MQ=255) gTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTa > 2:165427/1‑148 (MQ=255) cAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATc > 2:231742/1‑101 (MQ=255) cAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATc < 1:231742/101‑1 (MQ=255) gCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatg < 1:67633/125‑1 (MQ=255) gCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatg > 2:67633/1‑125 (MQ=255) ccTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCAACCTTACCGCTCTGGCGTATCAAGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGATATCTGgat > 4:367155/1‑147 (MQ=255) tctctTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtt > 3:176558/1‑132 (MQ=255) tctctTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtt < 4:176558/132‑1 (MQ=255) ctctTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGgaaga > 4:344863/1‑147 (MQ=255) tctTCCCCTGAAGGTTTCGAGCAAGATTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATACTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGgaagaa > 1:266051/1‑147 (MQ=255) ctTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTgg < 3:195301/133‑1 (MQ=255) ctTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTgg > 4:195301/1‑133 (MQ=255) tCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGAGGATGGATTTTGGCTATCTGGAAGAAACAt > 6:379496/1‑148 (MQ=255) ccccTGAAGGTTTCGAGCAATCTTCCGCTATTATTGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt < 2:178705/147‑1 (MQ=255) ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt < 5:405428/147‑1 (MQ=255) ccccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACAt < 6:112118/147‑1 (MQ=255) cccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTgg > 7:481/1‑83 (MQ=255) cccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTgg < 8:481/83‑1 (MQ=255) cacTGAAGGTTTCGAGCAAGCTTCGGCGATTATGGGCATCTTCTTCGTCAAATTTTTCATGTAGGGCATCCTTACCGCTCTGGCGTATCAGGTCGTCGTAGGTATTCGCCACATGATGATGGAttt < 6:78589/124‑1 (MQ=255) ccTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt > 5:78589/1‑126 (MQ=255) tGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatga < 2:180469/116‑1 (MQ=255) tGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatga > 1:180469/1‑116 (MQ=255) aaGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGctct > 3:120528/1‑76 (MQ=255) aaGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGctct < 4:120528/76‑1 (MQ=255) ttCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGt > 4:364789/1‑147 (MQ=255) tCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCATTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTa > 3:248483/1‑147 (MQ=255) cGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTg > 1:91084/1‑124 (MQ=255) cGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTg < 2:91084/124‑1 (MQ=255) aaGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTc > 8:247746/1‑148 (MQ=255) aGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgatg > 1:79611/1‑103 (MQ=255) aGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgatg < 2:79611/103‑1 (MQ=255) tCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGcc > 1:6501/1‑147 (MQ=255) tCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGcc > 3:33139/1‑147 (MQ=255) gcgATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCaaaa > 2:4372/1‑148 (MQ=255) gATTATGGGCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAAtc < 3:364789/148‑1 (MQ=255) gggCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCtt < 4:33139/147‑1 (MQ=255) ggCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTg > 6:62203/1‑148 (MQ=255) gCAGCTTCTTCGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGt < 7:247746/148‑1 (MQ=255) cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTaa < 5:269434/117‑1 (MQ=255) cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTaa > 6:269434/1‑117 (MQ=255) cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCaaa > 3:399123/1‑130 (MQ=255) cttcGTCAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCaaa < 4:399123/130‑1 (MQ=255) cAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTg > 5:264393/1‑148 (MQ=255) cAAATTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTg > 2:109784/1‑148 (MQ=255) aaaTTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGc < 3:344863/148‑1 (MQ=255) aTTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCtt < 1:165427/148‑1 (MQ=255) aTTTATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCtt > 6:339776/1‑148 (MQ=255) tATCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCa > 4:360063/1‑148 (MQ=255) aTCATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCa < 8:337231/147‑1 (MQ=255) cATGTAGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACtt > 5:222860/1‑148 (MQ=255) tgtaGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTg > 6:209908/1‑123 (MQ=255) tgtaGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTg < 5:209908/123‑1 (MQ=255) | TCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGAAGAAACATTCGAAGCGGGTAAACGCTCCGCCAAAATCTCCTTTGTTATTACTGTCGTGCTTTCACTT > NC_000913/755260‑755542 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |