Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,863,131 Δ1 bp 5.7% coding (361/996 nt) gapA → glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,1310G. 5.7% 241.8 / 7.1 70coding (361/996 nt)gapAglyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base . (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATC  >  NC_000913/1862989‑1863277
                                                                                                                                              |                                                                                                                                                  
tgtTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCCCCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCg                                                                                                                                               >  6:378207/1‑148 (MQ=255)
tgtTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCg                                                                                                                                               <  1:211384/148‑1 (MQ=255)
   tACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTc                                                                                                                                             >  6:286219/1‑147 (MQ=255)
     ccGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTaa                                                                                                                                          <  2:57756/148‑1 (MQ=255)
       gCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTCGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCCCCGCTGCTGCGAATAAAGTGGTTATGACTGGTCCGTCTAAAg                                                                                                                                        <  6:7292/148‑1 (MQ=255)
            aCGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGacaac                                                                                                                                   <  7:88262/148‑1 (MQ=255)
                   ccGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGa                                                                                                                            >  1:128277/1‑148 (MQ=255)
                          aCCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTTGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGt                                                                                                                     <  1:268313/148‑1 (MQ=255)
                          aCCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGt                                                                                                                     <  2:128277/148‑1 (MQ=255)
                           ccTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGt                                                                                                                     >  1:67334/1‑147 (MQ=255)
                               aaaTGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTaaa                                                                                                                 <  4:166081/147‑1 (MQ=255)
                               aaaTGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAg                                                                                                                >  5:364296/1‑148 (MQ=255)
                                 aTGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGgc                                                                                                              >  4:199647/1‑148 (MQ=255)
                                 aTGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGgc                                                                                                              >  4:193257/1‑148 (MQ=255)
                                         aaGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACtt                                                                                                      <  6:364296/148‑1 (MQ=255)
                                           gTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCg                                                                                                    <  1:298363/148‑1 (MQ=255)
                                           gTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCg                                                                                                    <  3:193257/148‑1 (MQ=255)
                                           gTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCg                                                                                                    <  3:199647/148‑1 (MQ=255)
                                             tGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGt                                                                                                                         <  7:382261/125‑1 (MQ=255)
                                             tGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGt                                                                                                                         >  8:382261/1‑125 (MQ=255)
                                              ggTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGAc                                                                                                  >  8:282745/1‑147 (MQ=255)
                                               gtgtTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTc                                                                                                                                             >  1:71060/1‑103 (MQ=255)
                                               gtgtTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTc                                                                                                                                             >  1:64683/1‑103 (MQ=255)
                                               gtgtTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTc                                                                                                                                             <  2:64683/103‑1 (MQ=255)
                                               gtgtTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTc                                                                                                                                             <  2:71060/103‑1 (MQ=255)
                                                tgtTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAACCAAATCTCCGCTGGTGCGAAGAAAGTTGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACaaa                                                                                               <  8:381320/148‑1 (MQ=255)
                                                 gtTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACaaa                                                                                               >  1:129288/1‑147 (MQ=255)
                                                   tGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAatat                                                                                            >  7:336948/1‑148 (MQ=255)
                                                        ttGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTgg                                                                                       <  5:286219/148‑1 (MQ=255)
                                                              cTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGa                                                                                 >  3:310447/1‑148 (MQ=255)
                                                              cTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGa                                                                                 >  4:202551/1‑147 (MQ=255)
                                                              cTGAAGCAACTGGTCGGGTCCTGACTGACGAAACTGCTCGTACACACATCACCGCTGGTGCGAAGAAAGGGGTTATGCCTGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGa                                                                                 >  4:196184/1‑147 (MQ=255)
                                                               tGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTaa                                                                                                                  >  3:410005/1‑114 (MQ=255)
                                                               tGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTaa                                                                                                                  <  4:410005/114‑1 (MQ=255)
                                                                    cAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGt                                                                           >  6:142625/1‑148 (MQ=255)
                                                                          gTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCaa                                                                     <  4:310447/148‑1 (MQ=255)
                                                                          gTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCa                                                                      <  7:282745/147‑1 (MQ=255)
                                                                           tCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACa                                                                               >  3:14749/1‑137 (MQ=255)
                                                                           tCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACa                                                                               <  4:14749/137‑1 (MQ=255)
                                                                                   tgactgacGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTg                                                            >  1:364528/1‑148 (MQ=255)
                                                                                        gacGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTg                                                            >  3:56043/1‑143 (MQ=255)
                                                                                        gacGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTg                                                            <  4:56043/143‑1 (MQ=255)
                                                                                          cGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGcaccac                                                      >  7:335581/1‑147 (MQ=255)
                                                                                          cGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGcaccac                                                      >  7:159627/1‑147 (MQ=255)
                                                                                          cGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGcaccac                                                      >  7:405437/1‑147 (MQ=255)
                                                                                            aaaCTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCaa                                                   <  2:129288/148‑1 (MQ=255)
                                                                                             aaCTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAAc                                                  >  2:155590/1‑148 (MQ=255)
                                                                                                      tAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCt                                         >  7:185026/1‑148 (MQ=255)
                                                                                                         acacATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCg                                      >  6:123083/1‑148 (MQ=255)
                                                                                                         acacATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCg                                      >  6:172939/1‑148 (MQ=255)
                                                                                                         acacATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCg                                      <  2:364528/148‑1 (MQ=255)
                                                                                                            caTCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGc                                               <  5:317566/136‑1 (MQ=255)
                                                                                                            caTCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGc                                               >  6:317566/1‑136 (MQ=255)
                                                                                                              tCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGc                                 >  7:150130/1‑148 (MQ=255)
                                                                                                                aCCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCt                                >  4:218446/1‑147 (MQ=255)
                                                                                                                 ccGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTaa                              <  8:150130/148‑1 (MQ=255)
                                                                                                                 ccGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTaa                              <  8:185026/148‑1 (MQ=255)
                                                                                                                 ccGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTa                               >  8:114413/1‑147 (MQ=255)
                                                                                                                       gTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTAt                        <  8:336948/148‑1 (MQ=255)
                                                                                                                       gTGCGAAGAAAGTGGTTATGACTGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTAt                        <  3:196184/147‑1 (MQ=255)
                                                                                                                       gTGCGAAGAAAGTGGTTATGACTGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTAt                        <  3:202551/147‑1 (MQ=255)
                                                                                                                       gTGCGAAGAAAGTGGTCATGACTGCTCCGTCTAAAGACAACACTCCGATGTGCGTTAAAGGCACTAACTTCGACAAATAAGCTGGCCAGGACCTCGTTCCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTAt                        <  4:209853/148‑1 (MQ=255)
                                                                                                                        tGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGt                           >  3:328670/1‑144 (MQ=255)
                                                                                                                        tGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGt                           <  4:328670/144‑1 (MQ=255)
                                                                                                                            aagaaAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAAc                    >  3:104509/1‑147 (MQ=255)
                                                                                                                            aagaaAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACg                   <  5:123083/148‑1 (MQ=255)
                                                                                                                               aaaGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATa                <  4:104509/148‑1 (MQ=255)
                                                                                                                                      ttATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACCTCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCgg         >  3:346437/1‑148 (MQ=255)
                                                                                                                                           aCTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCaa                                                   >  7:305538/1‑101 (MQ=255)
                                                                                                                                           aCTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCaa                                                   <  8:305538/101‑1 (MQ=255)
                                                                                                                                              ggTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCaa                     <  2:229330/128‑1 (MQ=255)
                                                                                                                                              ggTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCaa                     >  1:229330/1‑128 (MQ=255)
                                                                                                                                              ggTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCatcatc  >  1:61094/1‑147 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGTCTAAAGACAACACTCCGATGTTCGTTAAAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATC  >  NC_000913/1862989‑1863277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: