Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,272,209 A→G 6.1% I181I (ATT→ATC)  garR ← tartronate semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,272,2090AG 6.1% 198.4 / 3.1 66I181I (ATT→ATC) garRtartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base G (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATC  >  NC_000913/3272064‑3272350
                                                                                                                                                 |                                                                                                                                             
ccGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCCGTCCACCGCGAATTGCCTGATAACCCAGGTCCGGGTTAACGCCCGCGTTAGTTGCCAGCGTGACCGCGTCTGACATCGCGGCAatt                                                                                                                                            >  2:300066/1‑148 (MQ=255)
   gCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTc                                                                                                                                         <  1:29512/149‑1 (MQ=255)
       gAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAgc                                                                                                                                      <  5:397631/148‑1 (MQ=255)
          gTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCca                                                                                                                                  <  4:244068/149‑1 (MQ=255)
                     tCACCATCGGCGCTTTGGCATCCAGCACGTTACTGCCCGCCAGTCCACCGCTAATTGCCTGATAAACCATGTCCTGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGAAATCGCGGCCATATTCAGCGCCACAATGACCTGa                                                                                                                       >  4:33513/1‑149 (MQ=255)
                       aCCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATCAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGAtt                                                                                                                     <  8:383702/149‑1 (MQ=255)
                       aCCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGAt                                                                                                                      <  1:321912/148‑1 (MQ=255)
                        ccATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGAttt                                                                                                                    <  8:242699/149‑1 (MQ=255)
                        ccATCGGAGCTTTGGCATCCAGCACGGTACTGCCCGCGAGTCCACCGCGAATTGCCTGATACACCAGGTCCGGGTTAACGCCCGCGTTAGTTGCCTGCGTTTACGCGTCTTACATCGCGGCAATATTCAGCGCCACAATGACCTGAttt                                                                                                                    <  3:33513/149‑1 (MQ=255)
                                   ttGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCGATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGt                                                                                                         >  1:338040/1‑149 (MQ=255)
                                   ttGGCATCCAGCACGGTACTGCCCGCCAGTCCACAGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGATTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCGATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGt                                                                                                         >  1:332305/1‑149 (MQ=255)
                                      gCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTTCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGAc                                                                                                      <  1:378917/149‑1 (MQ=255)
                                         tCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCTGCAATATTCAGCGCCACAATGACCTGATTTGCAAGTTTGGTGACGt                                                                                                    >  6:302312/1‑148 (MQ=255)
                                         tCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGt                                                                                                    >  6:260593/1‑148 (MQ=255)
                                           cAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTAc                                                                                                 >  5:88715/1‑149 (MQ=255)
                                            aGCACGTTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTGCGGGGTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGGGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTAcc                                                                                                <  5:302312/149‑1 (MQ=255)
                                            aGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTAcc                                                                                                <  1:342684/149‑1 (MQ=255)
                                                     cTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGAt                                                                                       <  5:260593/149‑1 (MQ=255)
                                                     cTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGGGACGTTACCTGCACCGAt                                                                                       <  3:246984/149‑1 (MQ=255)
                                                      tGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGAtt                                                                                      >  4:393988/1‑149 (MQ=255)
                                                        cccGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCGCGCGTTAGTTGCCCGCGTTAACGCTACTGACATCGCGGCAATCATCAGCGCCACAAGGACCTGATTTGCCAGTTTGGTGCCGTTACCTGCACCGATTTc                                                                                    >  3:85559/1‑149 (MQ=255)
                                                         ccGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTcc                                                                                   >  3:89703/1‑149 (MQ=255)
                                                           gCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTcccc                                                                                 <  2:245207/149‑1 (MQ=255)
                                                                tCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTAt                                                                            >  8:22213/1‑149 (MQ=255)
                                                                tCCACCGCGAATTGCCTGATAAACCAGGTCCGGCTTAACGCCCGCTTTAGATCCCAGCGTTAACGCATCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCAAGTTTGGTGACGTTACCTGCACCTATTTCCCAGCTAt                                                                            >  8:210305/1‑149 (MQ=255)
                                                                 ccACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCATTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATg                                                                           <  1:300066/149‑1 (MQ=255)
                                                                    ccGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCCTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCTGATTGGTGACGTTACCTGCACCGATTTCCCCGGTATGcac                                                                        <  2:77458/149‑1 (MQ=255)
                                                                             gCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGcac                                                                        <  1:234216/140‑1 (MQ=255)
                                                                             gCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGcac                                                                        >  2:234216/1‑140 (MQ=255)
                                                                               cTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGAc                                                                                                      <  3:84547/108‑1 (MQ=255)
                                                                               cTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGAc                                                                                                      >  4:84547/1‑108 (MQ=255)
                                                                                tGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGc                                                            >  7:150656/1‑149 (MQ=255)
                                                                                 gATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGc                                                            >  1:46622/1‑148 (MQ=255)
                                                                                  aTAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGcc                                                           <  4:89703/148‑1 (MQ=255)
                                                                                  aTAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGcc                                                           >  8:196621/1‑148 (MQ=255)
                                                                                    aaaCCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATc                                                        >  8:105743/1‑149 (MQ=255)
                                                                                     aaCCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCg                                                       >  7:128796/1‑149 (MQ=255)
                                                                                          ggTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtc                                                  <  7:196621/149‑1 (MQ=255)
                                                                                           gTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCGCCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtca                                                 >  5:255466/1‑149 (MQ=255)
                                                                                           gTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtca                                                 >  2:136072/1‑149 (MQ=255)
                                                                                              cGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtcatc                                               <  7:105743/148‑1 (MQ=255)
                                                                                                     aCGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGCCCTGATTTGCCAGTTTGGTGACGTTACCTGCCCCGATATCCCCGGTATGGACCACGGAACCCGCCATCGCTTTCATCAAAtga                                         >  4:146545/1‑145 (MQ=255)
                                                                                                          cGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtca                                                 <  5:362843/134‑1 (MQ=255)
                                                                                                          cGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtca                                                 >  6:362843/1‑134 (MQ=255)
                                                                                                           gCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTAttt                                 >  7:388462/1‑149 (MQ=255)
                                                                                                                 gTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa                           <  6:255466/149‑1 (MQ=255)
                                                                                                                  ttGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGt                                     <  1:262724/138‑1 (MQ=255)
                                                                                                                  ttGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGt                                     >  2:262724/1‑138 (MQ=255)
                                                                                                                    gCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaaa                         <  8:388462/148‑1 (MQ=255)
                                                                                                                      cAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaaa                         >  5:137206/1‑146 (MQ=255)
                                                                                                                      cAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATa                       <  6:137206/148‑1 (MQ=255)
                                                                                                                         cGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCt                   <  2:46622/149‑1 (MQ=255)
                                                                                                                          gTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt                  <  8:128796/149‑1 (MQ=255)
                                                                                                                              aCGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTc               <  3:393988/148‑1 (MQ=255)
                                                                                                                               cGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTcgc             <  7:22213/149‑1 (MQ=255)
                                                                                                                                gCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTcgcc            >  4:193502/1‑149 (MQ=255)
                                                                                                                                  ttCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCCCGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTcgccgc          >  8:371108/1‑149 (MQ=255)
                                                                                                                                   tCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCa                                         <  7:358554/117‑1 (MQ=255)
                                                                                                                                   tCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCa                                         >  8:358554/1‑117 (MQ=255)
                                                                                                                                    cTGACATCGCGGCGATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGTTATGAACAACGGAACCCGCCATCGCTTTAATCAAATCATATTATTTGGCGAAAATAGCCTTGTCgcagcc         <  2:332305/148‑1 (MQ=255)
                                                                                                                                    cTGACATCGCGGCGATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCgccgcc         <  2:338040/148‑1 (MQ=255)
                                                                                                                                      gACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGccccc      >  5:194893/1‑147 (MQ=255)
                                                                                                                                      gACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCccac      >  5:22725/1‑149 (MQ=255)
                                                                                                                                         aTCGCGGCAATATTCCCCTCCACAATGACCTGATTTGCCAGCGTGGTGACGTGACCTGCACCGATTTCCCCGCTCTGCACCACGGATCCCGCCATCGCGTTCATCAAATCATAGTATATGTCGAAACTAGCCTTCTCGCCGCCCACCAt   <  4:85559/149‑1 (MQ=255)
                                                                                                                                          tCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCAt   >  7:324450/1‑148 (MQ=255)
                                                                                                                                          tCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATc  >  3:285964/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATC  >  NC_000913/3272064‑3272350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑