Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,216,925 A→G 5.7% P161P (CCT→CCC yehX ← putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,216,9250AG 5.7% 268.4 / 3.8 87P161P (CCT→CCCyehXputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (37/44);  new base G (3/2);  total (40/47)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAGCGCCATTAATTCGTCGATACGA  >  NC_000913/2216779‑2217072
                                                                                                                                                  |                                                                                                                                                   
tGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAgg                                                                                                                                                   >  7:72892/1‑149 (MQ=255)
    ccATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCa                                                                                                                                               >  3:59129/1‑149 (MQ=255)
      aTCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATc                                                                                                                                             <  5:372897/149‑1 (MQ=255)
        cAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCGAAAAGGTTCATCCa                                                                                                                                           <  2:196052/149‑1 (MQ=255)
         aaTACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCAt                                                                                                                                          >  7:314782/1‑149 (MQ=255)
         aaTACCAGATGTGCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCAt                                                                                                                                          <  2:197658/149‑1 (MQ=255)
          aTACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCAt                                                                                                                                          <  7:257081/148‑1 (MQ=255)
          aTACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCAt                                                                                                                                          >  8:257081/1‑148 (MQ=255)
           tACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTa                                                                                                                                        <  8:146465/149‑1 (MQ=255)
               aGATGTTCTGCCCGCCGTAGCGCCTCATCAATATCATGAGTGACCATCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATATCTTGTTGCATCGCGGCGCGCGTTACCGGGTCCAGGTCGCCAAAAGGTTCATCCATTAGTaa                                                                                                                                    <  8:92574/149‑1 (MQ=255)
                  tGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGAc                                                                                                                                 <  5:380244/149‑1 (MQ=255)
                   gTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACt                                                                                                                                >  8:119779/1‑149 (MQ=255)
                    ttCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTa                                                                                                                                        >  4:172033/1‑140 (MQ=255)
                    ttCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTa                                                                                                                                        <  3:172033/140‑1 (MQ=255)
                     tCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACAGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAgcc                                                                                                                                 >  2:203376/1‑144 (MQ=255)
                     tCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACAGGGGCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGAc                                                                                                                                 <  1:203376/146‑1 (MQ=255)
                      cTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTg                                                                                                                              <  8:379511/148‑1 (MQ=255)
                         ccAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGc                                                                                                                             <  1:38219/146‑1 (MQ=255)
                           aGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATc                                                                                                                        <  7:70103/149‑1 (MQ=255)
                           aGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAAAGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGAAAGACTAGCGGATc                                                                                                                        >  7:210812/1‑149 (MQ=255)
                                   cgcCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCa                                                                                                                <  5:382628/149‑1 (MQ=255)
                                   cgcCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGAAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCa                                                                                                                <  4:59129/149‑1 (MQ=255)
                                    gcCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAg                                                                                                               >  6:26285/1‑149 (MQ=255)
                                          tcaATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCa                                                                                                                                           <  6:190321/115‑1 (MQ=255)
                                          tcaATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCa                                                                                                                                           >  5:190321/1‑115 (MQ=255)
                                          tcaATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCAcg                                                                                                         <  8:73906/149‑1 (MQ=255)
                                            aaTATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCCGTGCGCCAAAAGGTTCATCCAGTAGTAAGAGTTGCGGATCGGCAGCCAGTGCAcgcg                                                                                                       >  2:320566/1‑149 (MQ=255)
                                             atatCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACgcgc                                                                                                      <  7:119779/149‑1 (MQ=255)
                                             atatCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACgcgc                                                                                                      <  4:275206/149‑1 (MQ=255)
                                                    gAGTGACCAGCACAATGGTACGCCCCAGCACACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGAAAGACATGCGGATCGGCACCCAGTGCACCCGCCACTcc                                                                                                >  5:173076/1‑148 (MQ=255)
                                                       tGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAGGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCcaca                                                                                            >  8:381454/1‑149 (MQ=255)
                                                       tGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAGGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCcaca                                                                                            <  7:375803/149‑1 (MQ=255)
                                                       tGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAGGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCcaca                                                                                            <  7:381454/149‑1 (MQ=255)
                                                       tGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAGGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCcaca                                                                                            >  8:375803/1‑149 (MQ=255)
                                                          ccAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGt                                                                                         >  5:122316/1‑149 (MQ=255)
                                                          ccAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGt                                                                                         >  6:225595/1‑149 (MQ=255)
                                                           cAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTACCACACGtt                                                                                        >  6:147343/1‑149 (MQ=255)
                                                               acaATGGTACGCCCCAGCAAACGGTGAATGCGCGTCCTCTCTTGTTGCAACGCGCCGCTCGTTACCGGGGCCAGTGCGCCAAACGGTGCATCCATTCGTAAGACATGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgctg                                                                                    <  8:306070/149‑1 (MQ=255)
                                                               acaATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgctg                                                                                    <  5:147343/149‑1 (MQ=255)
                                                                caATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgctgc                                                                                   <  8:72892/149‑1 (MQ=255)
                                                                 aaTGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTgctgct                                                                                  <  8:51496/149‑1 (MQ=255)
                                                                 aaTGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTgctgct                                                                                  >  3:393559/1‑149 (MQ=255)
                                                                   tGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGa                                                                                <  2:62316/149‑1 (MQ=255)
                                                                       aCGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGcccac                                                                            >  6:274611/1‑149 (MQ=255)
                                                                         gCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgc                                                                                      >  5:408073/1‑137 (MQ=255)
                                                                         gCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgc                                                                                      <  6:408073/137‑1 (MQ=255)
                                                                            ccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGTGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGaa                                                                       <  8:314782/149‑1 (MQ=255)
                                                                            ccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGc                                                                                                                             <  1:394835/95‑1 (MQ=255)
                                                                            ccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGc                                                                                                                             <  1:388959/95‑1 (MQ=255)
                                                                            ccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGc                                                                                                                             >  2:394835/1‑95 (MQ=255)
                                                                            ccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGc                                                                                                                             >  2:388959/1‑95 (MQ=255)
                                                                            ccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGa                                                                        >  2:65257/1‑148 (MQ=255)
                                                                                 aaaCGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCCCGCGCCACTCCCACACGTTGCAGCTGACCACCGGAAAGCt                                                                   >  1:110287/1‑148 (MQ=255)
                                                                                  accGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGa                                                                 <  1:61586/147‑1 (MQ=255)
                                                                                  aaCGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGa                                                                 <  5:180490/149‑1 (MQ=255)
                                                                                      gTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAGGGTTCGGCCAGTAGGAGGACTGGGGGATCGGCGGCCAGTGCGCGCGGCACTCCCACAGGTTGCGGCTGACCCCCGGAAAGCTGATGCg                                                             >  4:95564/1‑149 (MQ=255)
                                                                                      gTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCg                                                             >  8:328143/1‑149 (MQ=255)
                                                                                       tGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCgg                                                            <  5:274611/149‑1 (MQ=255)
                                                                                        gAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCgg                                                            >  2:141640/1‑148 (MQ=255)
                                                                                         aaTGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtg                                                                                       >  3:319330/1‑120 (MQ=255)
                                                                                         aaTGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtg                                                                                       <  4:319330/120‑1 (MQ=255)
                                                                                                gTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCt                                                    >  8:342432/1‑148 (MQ=255)
                                                                                                gTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTc                                                   <  1:141640/149‑1 (MQ=255)
                                                                                                   aTCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGc                                                                                                                             >  7:11882/1‑72 (MQ=255)
                                                                                                   aTCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGc                                                                                                                             <  8:11882/72‑1 (MQ=255)
                                                                                                    tctcTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACg                                                >  4:242946/1‑148 (MQ=255)
                                                                                                     ctctTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGc                                               >  1:172101/1‑148 (MQ=255)
                                                                                                          gttgCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAAttt                                         >  1:298547/1‑149 (MQ=255)
                                                                                                          gttgCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAAtt                                          <  5:26285/148‑1 (MQ=255)
                                                                                                          gttgCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAAtt                                          <  3:95564/148‑1 (MQ=255)
                                                                                                          gttgCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAAt                                           <  5:225595/147‑1 (MQ=255)
                                                                                                           ttgCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAAttt                                         >  7:40477/1‑148 (MQ=255)
                                                                                                           ttgCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTg                                        >  8:213201/1‑149 (MQ=255)
                                                                                                             gCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGa                                       <  8:40477/148‑1 (MQ=255)
                                                                                                             ccAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCAAGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGa                                       >  2:205020/2‑148 (MQ=255)
                                                                                                              cAACGCGCCGCGCGTTACCGCGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTACCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACt                                     >  6:381890/1‑149 (MQ=255)
                                                                                                                aCGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACTcc                                   <  3:242946/149‑1 (MQ=255)
                                                                                                                    gccgcgcGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCg                                                                          <  4:7303/106‑1 (MQ=255)
                                                                                                                           gTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAg                        <  2:38682/149‑1 (MQ=255)
                                                                                                                                cGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAGCGCCa                   >  3:214948/1‑149 (MQ=255)
                                                                                                                                cGGGGCCAGGGCGCCAAAAGGTTCATCCATTCGTAACACTTGCGCATCGGCAGCCAGGGAACGCGCCACACCCACACGTTGCTGCTTACAACCGCAAAGGTTATGCGTAAAACGCTCACCCAAATTTGACTCCTGCCCAGTAGCGCCAt                  <  4:393559/149‑1 (MQ=255)
                                                                                                                                 ggggCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCAGACGTTGCTGCTGACCACCGGATAGCTGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAGCGcctt                  <  6:173076/149‑3 (MQ=255)
                                                                                                                                        gTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtg                                                                                       <  6:240805/73‑1 (MQ=255)
                                                                                                                                         tGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGa                                                                                <  3:95/79‑1 (MQ=255)
                                                                                                                                         tGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGa                                                                                >  4:95/1‑79 (MQ=255)
                                                                                                                                            gcCAAAAGCTTCATCCATTATTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAGCGCCATTAATTCGTCGa       <  8:210812/149‑1 (MQ=255)
                                                                                                                                            gcCAAAAGCTTCATCCATAAGGAAGACTTGCTGCTCGGCAGCCAGTGCGCGCGCCACTCCCACACGTTGCTGCTGCCGCCCGGTAAGCGGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAGCGCCATTAATTCGTCGa       >  2:152672/1‑149 (MQ=255)
                                                                                                                                                 aaGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAGCGCCATTAATTCGTCGATACGa  >  7:71138/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATTTGACTCCAGCCCCAGTAGCGCCATTAATTCGTCGATACGA  >  NC_000913/2216779‑2217072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: