Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,141,656 T→A 7.3% E904D (GAA→GAT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,6560TA 7.3% 188.5 / 2.7 66E904D (GAA→GAT) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base A (3/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAAC  >  NC_000913/1141511‑1141802
                                                                                                                                                 |                                                                                                                                                  
ctTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCg                                                                                                                                                 >  1:198905/1‑149 (MQ=255)
 ttCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATAAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCgg                                                                                                                                                >  8:374360/1‑149 (MQ=255)
   ccTGCGGTTCAACCACCGCTTCTGCTTGTTCTGCAACTTGCTGCGGTACGGAAACATCAGACTCTGTAATCTCCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCACGTTGCGTCGTTTCAACCCCTTCCGGTTGCGGATCg                                                                                                                                                 <  6:299493/146‑1 (MQ=255)
       cGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAAcc                                                                                                                                           <  8:245270/148‑1 (MQ=255)
       cGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCt                                                                                                                                          <  8:350345/149‑1 (MQ=255)
          ttCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCCGAACCGCAACCGGaa                                                                                                                                       <  4:275947/149‑1 (MQ=255)
              accaccGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACggg                                                                                                                                   <  4:184470/149‑1 (MQ=255)
               ccaccGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACgggg                                                                                                                                  >  1:139517/1‑149 (MQ=255)
                   cGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGAATCGGTAATCACCTGCGTCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCTCTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCtt                                                                                                                              <  2:73600/149‑1 (MQ=255)
                    ggTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCACACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTc                                                                                                                             <  2:198905/149‑1 (MQ=255)
                           cTTGTTCTGCAAATTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGTCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAAACTGAACGGGGGCTTCTACGa                                                                                                                        <  3:42542/147‑1 (MQ=255)
                              gTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTGCGGCAACCTGAACGGGGGCTTCTACGACAcc                                                                                                                    <  4:316399/148‑1 (MQ=255)
                                  tGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCa                                                                                                               >  3:361357/1‑149 (MQ=255)
                                            tGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGc                                                                                                                                               <  4:181181/107‑1 (MQ=255)
                                            tGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGc                                                                                                                                               >  3:181181/1‑107 (MQ=255)
                                            tGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACAGCGGCAGAGATCACTTTAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTcaa                                                                                                      >  6:41033/1‑148 (MQ=255)
                                                       aaCATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTACGGCAACCAGATCGGGGGCTTCTACGACACCGGCCACGTCTTCAACAACTGgcgcg                                                                                           >  2:126624/1‑148 (MQ=255)
                                                       aaCATCAGACTCGGTAATCACCTGCGGCTGCTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGgcgcgc                                                                                          >  3:79686/1‑149 (MQ=255)
                                                         cATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCt                                                                                         >  7:387381/1‑148 (MQ=255)
                                                         cATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTa                                                                                        <  4:229085/149‑1 (MQ=255)
                                                          aTCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGaa                                                                                                                                       >  7:204237/1‑101 (MQ=255)
                                                          aTCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGaa                                                                                                                                       <  8:204237/101‑1 (MQ=255)
                                                          aTCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTaa                                                                                       >  5:331761/1‑149 (MQ=255)
                                                          aTCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTGCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTaa                                                                                       <  6:163948/149‑1 (MQ=255)
                                                             aGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGaca                                                                                                                      >  3:352039/1‑115 (MQ=255)
                                                             aGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGaca                                                                                                                      <  4:352039/115‑1 (MQ=255)
                                                             aGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTaacaa                                                                                    <  4:361357/149‑1 (MQ=255)
                                                                 tCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGgc                                                                                              <  8:19972/135‑1 (MQ=255)
                                                                 tCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGgc                                                                                              >  7:19972/1‑135 (MQ=255)
                                                                 tCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCgg                                                                                >  3:165742/1‑149 (MQ=255)
                                                                    gTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTc                                                                             <  6:331761/149‑1 (MQ=255)
                                                                    gTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTGCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTc                                                                             <  2:139517/149‑1 (MQ=255)
                                                                        tCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGa                                                                           >  5:76811/1‑147 (MQ=255)
                                                                        tCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGa                                                                           >  3:185665/1‑147 (MQ=255)
                                                                          aCCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAgc                                                                       <  2:142850/149‑1 (MQ=255)
                                                                            cTGAGGCTGGTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACCACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAgcgg                                                                     >  5:204419/1‑149 (MQ=255)
                                                                                ggCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAgcggcggc                                                                 <  7:374360/149‑1 (MQ=255)
                                                                                     ttCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAg                                                            <  2:427967/149‑1 (MQ=255)
                                                                                      gcAGGTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGGTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAgg                                                           <  1:126624/148‑1 (MQ=255)
                                                                                                     gagATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCAt                                            <  4:79686/147‑1 (MQ=255)
                                                                                                       gATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCg                                          >  3:56019/1‑149 (MQ=255)
                                                                                                        aTCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGtt                                                                              <  1:69148/112‑1 (MQ=255)
                                                                                                        aTCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGtt                                                                              >  2:69148/1‑112 (MQ=255)
                                                                                                        aTCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGC‑TCTACGACACCGGCCACTTGTTCAACAACTGGCGCGCTTACAACCCGTTAGATAGCGGCGGCGACAGGGACCTCCGTCCCCCTCCGc                                        >  4:148533/1‑149 (MQ=255)
                                                                                                             ttCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGca                                    >  3:188321/1‑149 (MQ=255)
                                                                                                               ccggATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGca                                    <  4:225164/145‑1 (MQ=255)
                                                                                                               cAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGacac                                                                                                                     <  7:244910/66‑1 (MQ=255)
                                                                                                               cAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGacac                                                                                                                     >  8:244910/1‑66 (MQ=255)
                                                                                                                       gtcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTActtc                          >  7:22272/1‑149 (MQ=255)
                                                                                                                       gtcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTActtc                          >  5:194660/1‑149 (MQ=255)
                                                                                                                        tcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTActtct                         >  5:242888/1‑149 (MQ=255)
                                                                                                                        tcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTActtct                         <  6:76811/149‑1 (MQ=255)
                                                                                                                            ttCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCt                     >  8:6211/1‑149 (MQ=255)
                                                                                                                                ccccgttCAGGTTGCCGAACCGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTc                 >  5:80680/6‑149 (MQ=255)
                                                                                                                                ccccgttCAGGTTGCCGAACCGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTc                 >  3:84841/6‑149 (MQ=255)
                                                                                                                                  cACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTcgc               >  5:23836/1‑149 (MQ=255)
                                                                                                                                  cACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTcgc               >  4:136320/1‑149 (MQ=255)
                                                                                                                                    cTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCgcgc             >  2:86103/1‑149 (MQ=255)
                                                                                                                                    cTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCgcgc             <  4:165742/149‑1 (MQ=255)
                                                                                                                                     ttCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTTCGACACCGGCCACTTCTTCAACAACt                                                                                                 <  8:330416/64‑1 (MQ=255)
                                                                                                                                     ttCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACt                                                                                                 >  7:330416/1‑64 (MQ=255)
                                                                                                                                     ttCAGGTTGCCGAACCGCAACCGGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCgct            >  4:393687/1‑149 (MQ=255)
                                                                                                                                         ggTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGctc        >  6:174530/1‑149 (MQ=255)
                                                                                                                                             gCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTc     <  3:136320/148‑1 (MQ=255)
                                                                                                                                              cGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCaa   <  5:41033/149‑1 (MQ=255)
                                                                                                                                               ggTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAAc  >  5:217716/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACTTCCTGGGCTACGGCAACATCAGACTCGGTAATCACCTGCGGCTGTTCAGTTACCGCGGCAGCGATCACTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCTGCTCTTCAAC  >  NC_000913/1141511‑1141802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑