Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,239	C→A	T498K (ACG→AAG)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,239	0	C	A	100.0%	97.8 / NA	30	T498K (ACG→AAG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/14);  total (16/14)

GAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCATGCTGCTGCCG  >  NC_000913/2416115‑2416384                                                                                                                             |                                                                                                                                                  gAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGt                                                                                                                           >  6:141356/1‑149 (MQ=255)   aaTCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACa                                                                                                                                    >  5:42113/1‑138 (MQ=255)   aaTCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACa                                                                                                                                    <  6:42113/138‑1 (MQ=255)           aTCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTg                                                                                                                 >  6:85996/1‑149 (MQ=255)                    tGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGt                                                                                                        <  5:141356/149‑1 (MQ=255)                                            gtcTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAacac                                                                                 >  2:30902/1‑148 (MQ=255)                                            gtcTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAacac                                                                                 <  5:85996/148‑1 (MQ=255)                                                ggTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACCGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACCCTACCGCAAACACCAtcc                                                                            >  8:117854/1‑149 (MQ=255)                                                 gTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCAtccg                                                                           >  2:156411/1‑149 (MQ=255)                                                        tGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATg                                                                                                                      >  8:82884/1‑99 (MQ=255)                                                        tGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATg                                                                                                                      <  7:82884/99‑1 (MQ=255)                                                          cGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCt                                                                  >  5:44039/1‑149 (MQ=255)                                                           gTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCAt                                                                              >  8:51600/1‑136 (MQ=255)                                                           gTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCAt                                                                              <  7:51600/136‑1 (MQ=255)                                                                  cAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGGGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGCGGGTCTGGTTTCCGGGGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGa                                                          <  7:117854/149‑1 (MQ=255)                                                                        cATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCaaaa                                                    <  1:30902/149‑1 (MQ=255)                                                                         aTGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTc                                                                                                            >  4:44254/1‑92 (MQ=255)                                                                         aTGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTc                                                                                                            <  3:44254/92‑1 (MQ=255)                                                                              cGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCAc                                              >  7:34902/1‑149 (MQ=255)                                                                                          ccGCGAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTccc                                  >  5:115752/1‑149 (MQ=255)                                                                                            gcgAACAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTg                                <  6:44039/149‑1 (MQ=255)                                                                                                aCAGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTAt                            <  6:115752/149‑1 (MQ=255)                                                                                                  aGCTGGAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCt                          >  8:89655/1‑149 (MQ=255)                                                                                                       gAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTcc                       <  1:156411/147‑1 (MQ=255)                                                                                                       gAAGACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCgt                     >  6:97418/1‑149 (MQ=255)                                                                                                          gACAACGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGtt                  <  5:97418/149‑1 (MQ=255)                                                                                                           aCAACGGGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAACATGATGGTCTGGTTTCCGGTGCTGGTCACACAACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCgaggtc                 >  8:54580/1‑144 (MQ=255)                                                                                                               cGTGGTGCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCa             <  8:34902/149‑1 (MQ=255)                                                                                                                 tggtgCTCGGTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACAGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAtg           >  2:86445/1‑149 (MQ=255)                                                                                                                          gTAAGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGTTATCTTCCGTGTTCTTCATGCTGCTGCCg  <  1:86445/149‑1 (MQ=255)                                                                                                                             |                                                                                                                                                  GAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCATGCTGCTGCCG  >  NC_000913/2416115‑2416384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑