Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,158,626	G→A	G253D (GGC→GAC)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,158,626	0	G	A	100.0%	225.9 / NA	70	G253D (GGC→GAC)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/36);  total (34/36)

CTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGATC  >  NC_000913/1158481‑1158768                                                                                                                                                  |                                                                                                                                               cTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGTTGCAGATTGGTGAATACACCAACGCAGCAGGGCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACtt                                                                                                                                             <  1:203985/149‑1 (MQ=255)  tGGTACCGTTTGGTCTGCCCCACATCTGGAACGTACCTTTCCAGATGCAGCTTGGTGAATACACCCACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTc                                                                                                                                            >  2:236509/1‑149 (MQ=255)        cGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGTTTTTCCAAGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTACTGtt                                                                                                                                       >  5:45/1‑148 (MQ=255)             ggTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCaaaa                                                                                                                                  <  7:112115/148‑1 (MQ=255)              gTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACTCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAAt                                                                                                                                 <  1:146740/148‑1 (MQ=255)              gTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAAt                                                                                                                                 <  2:178082/148‑1 (MQ=255)                  gCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACg                                                                                                                            >  5:95886/1‑149 (MQ=255)                   caccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGt                                                                                                                               <  5:22097/145‑1 (MQ=255)                   caccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGt                                                                                                                               >  6:22097/1‑145 (MQ=255)                     ccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTc                                                                                                                         >  7:148633/1‑149 (MQ=255)                     ccacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATACCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTc                                                                                                                         >  2:43520/1‑149 (MQ=255)                      cacaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCt                                                                                                                        >  5:139882/1‑149 (MQ=255)                      cacaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCt                                                                                                                        >  5:248789/1‑149 (MQ=255)                       acaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGGATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTg                                                                                                                       >  3:325747/1‑149 (MQ=255)                       acaTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGGTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTg                                                                                                                       >  2:288581/1‑149 (MQ=255)                       aaaTCTGGAACGAACCTTTCCAGATGCAGATTGGTCACTACACCAACGCAGCAGGTCAGGTTAACCACGGCCACATTCACCGATATATGGCGCGTGACCCGAATGCGGGTAAACTGTCTGGTGACTTCCTCTGCTAAATGTTCGGACta                                                                                                                       >  2:336302/3‑148 (MQ=255)                            tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGGGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGc                                                                                                                  >  6:284894/1‑149 (MQ=255)                            tGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGc                                                                                                                  >  1:357349/1‑149 (MQ=255)                              gAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCa                                                                                                                    <  5:162260/145‑1 (MQ=255)                              gAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCa                                                                                                                    <  2:30823/145‑1 (MQ=255)                              gAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTg                                                                                                                >  3:122804/1‑149 (MQ=255)                               aaCGTACCTTTCCAGATGCAGTTTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCTGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGc                                                                                                               <  8:374721/149‑1 (MQ=255)                               aaCGTACCTTTCCAGATGCAGATTGGTGAATACTCCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAATCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGc                                                                                                               <  7:277049/149‑1 (MQ=255)                                   tACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGc                                                                                                            >  4:106222/1‑148 (MQ=255)                                    aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa                                                                                                          >  4:140768/1‑149 (MQ=255)                                      cTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCTGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTACTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAtt                                                                                                        >  8:205404/1‑149 (MQ=255)                                      cTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAtt                                                                                                        <  8:213262/149‑1 (MQ=255)                                         tCCAGATGCAGATTGGTGAATACACCAACGCAGCATGGCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACACGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                                                                                       <  6:45/147‑1 (MQ=255)                                              aTGCAGATTGGTGAATACCCCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGCCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTg                                                                                                >  3:151056/1‑149 (MQ=255)                                                gCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTgg                                                                                               >  3:84334/1‑148 (MQ=255)                                                gCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTgg                                                                                               >  6:156959/1‑148 (MQ=255)                                                 cAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGTGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                                                                                             <  5:284894/149‑1 (MQ=255)                                                 cAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGc                                                                                              <  6:95886/148‑1 (MQ=255)                                                   gATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCAct                                                                                           <  7:387625/149‑1 (MQ=255)                                                        gTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGc                                                                                       >  2:95968/1‑148 (MQ=255)                                                         tGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCt                                                                                      >  4:55386/1‑148 (MQ=255)                                                              acacCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCaaaa                                                                                                                                  <  3:382376/99‑1 (MQ=255)                                                              acacCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCaaaa                                                                                                                                  >  4:382376/1‑99 (MQ=255)                                                                acCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATATGGCACTCTGCTAAACCAg                                                                               >  2:406752/1‑148 (MQ=255)                                                                   aaCGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaaaa                                                                           <  1:247511/149‑1 (MQ=255)                                                                     cGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTa                                                                                                                              >  4:245324/1‑96 (MQ=255)                                                                     cGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTa                                                                                                                              <  3:245324/96‑1 (MQ=255)                                                                     cGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAAcc                                                                         <  4:84334/149‑1 (MQ=255)                                                                     cGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAAcc                                                                         <  5:355237/149‑1 (MQ=255)                                                                             gtcaggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACTGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGaaa                                                                 <  6:139882/149‑1 (MQ=255)                                                                             gtcaggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAGCCGCGCGa                                                                   >  1:39493/1‑147 (MQ=255)                                                                              tcaggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAg                                                                >  6:330903/1‑149 (MQ=255)                                                                                aggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTg                                                              <  2:120437/149‑1 (MQ=255)                                                                                   ttttCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGc                                                           <  5:156959/149‑1 (MQ=255)                                                                                     ttCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCg                                                          <  2:357349/148‑1 (MQ=255)                                                                                       ccACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTaaa                                                                                   >  1:384525/1‑121 (MQ=255)                                                                                       ccACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTaaa                                                                                   <  2:384525/121‑1 (MQ=255)                                                                                        cACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGta                                                       <  4:325747/148‑1 (MQ=255)                                                                                           ggCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGtattat                                                   <  1:95968/149‑1 (MQ=255)                                                                                              gACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGAt                                                <  4:122804/149‑1 (MQ=255)                                                                                                  ttCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGt                                                               <  8:79307/130‑1 (MQ=255)                                                                                                  ttCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGt                                                               >  7:79307/1‑130 (MQ=255)                                                                                                       cgTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCgcggc                                       <  3:106222/149‑1 (MQ=255)                                                                                                         ttATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGGCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGgcgc                                     <  1:43520/149‑1 (MQ=255)                                                                                                               ggCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCt                               >  6:222343/1‑149 (MQ=255)                                                                                                                    ttGACCAGTCTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCAAGCTGGCGCAATTGCTATCTTGCACTCTGCTTAACCAGAAAACCGTGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTc                          <  4:151056/148‑1 (MQ=255)                                                                                                                    gTGACCCTACTGCGGTTAAACTGTCTTGTGACTTCCTGTTCAAAATGTACGGTCTACCAGCTGCCGCAATTGCTTTCTGGCACTCTGCTTAACGAGAAATGCGCGGGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTc                          <  1:336302/149‑1 (MQ=255)                                                                                                                    gTGACCCGACTGCGGGTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTc                          <  7:205404/149‑1 (MQ=255)                                                                                                                                 gggTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCa             >  7:268560/1‑149 (MQ=255)                                                                                                                                 gggTAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCa             >  4:304615/1‑149 (MQ=255)                                                                                                                                  ggTAAACTGTCTGGTGACTGCCGGTTCAAAATGTACGGTCTGCCAGCTGCCGCATTTGCTATCGGGCACTCTGCTAAGCCAGAAACCCGCGCGCAAGTGGGCGGTAGTAGGCTCGCCGCGGCGCTGACCTCGGTCGGGCCCGGTATctc            >  8:298871/1‑147 (MQ=255)                                                                                                                                    tAAACTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCg          <  5:222343/149‑1 (MQ=255)                                                                                                                                      aaCTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGa         <  1:288581/148‑1 (MQ=255)                                                                                                                                        cTGTCTGGTGACTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGcc      >  3:26654/1‑149 (MQ=255)                                                                                                                                           tCTGGTGACTTCCTGTTCAAAATGTACGGGCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGAt   <  8:148633/149‑1 (MQ=255)                                                                                                                                             tggtgaCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGa    >  1:396379/1‑146 (MQ=255)                                                                                                                                             tggtgaCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGATc  >  4:170890/1‑148 (MQ=255)                                                                                                                                               gtgaCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGATc  <  4:174284/146‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               CTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTATGATCTCCGCGGCGCTGACCTCGTTCCTGACCGGTATCACCGAGCCGATC  >  NC_000913/1158481‑1158768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑