Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 909,400 C→T M550I (ATG→ATA poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913909,4000CT97.1% 225.3 / NA 69M550I (ATG→ATApoxBpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (30/36);  minor base G (0/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTT  >  NC_000913/909255‑909545
                                                                                                                                                 |                                                                                                                                                 
ggCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGtata                                                                                                                                                <  6:340328/149‑1 (MQ=255)
     ggTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATAc                                                                                                                                             <  5:390334/147‑1 (MQ=255)
     ggTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACa                                                                                                                                            <  1:189329/148‑1 (MQ=255)
     ggTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACa                                                                                                                                            <  6:317396/148‑1 (MQ=255)
          ggTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGa                                                                                                                                      <  8:193766/149‑1 (MQ=255)
            tAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGaaa                                                                                                                                    <  1:153524/149‑1 (MQ=255)
                 gTATCGCTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCttt                                                                                                                               <  2:257691/149‑1 (MQ=255)
                 gTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCttt                                                                                                                               <  7:77383/149‑1 (MQ=255)
                 gTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACACTTTATACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCTCGACTGAATGCGCGCAGTATCTACAGGCTGACACCttt                                                                                                                               >  6:204332/1‑149 (MQ=255)
                  tATCACTGCGTAAATCTATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTg                                                                                                                              <  1:207512/149‑1 (MQ=255)
                  tATCACTGCGGAAATCTCTCATGGCCTGTCGTTATTATGACGGGAAATGCCACCCTTTTTACCTTCGCCAGTTTGTTTTCGCCAGTTCGATCTCTTCATCCCCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGTAAGCTTTg                                                                                                                              <  2:356037/149‑1 (MQ=255)
                     cACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGATATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGc                                                                                                                            <  3:343694/148‑1 (MQ=255)
                        tGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACACTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGt                                                                                                                        >  6:100039/1‑149 (MQ=255)
                          cGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCATTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCTCTGATGATTGCGCGCAGTATATACAGGCTTAAACCTTTGGCCTGTTc                                                                                                                      >  3:52028/1‑149 (MQ=255)
                          cGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTc                                                                                                                      <  5:285789/149‑1 (MQ=255)
                            tAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGt                                                                                                                        <  5:193702/145‑1 (MQ=255)
                            tAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGt                                                                                                                        >  6:193702/1‑145 (MQ=255)
                            tAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGAGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGa                                                                                                                    >  5:214383/1‑149 (MQ=255)
                               atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCAGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt                                                                                                                 <  4:39280/149‑1 (MQ=255)
                                 caatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGttt                                                                                                                >  7:352417/1‑148 (MQ=255)
                                  aatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTg                                                                                                               <  4:137565/148‑1 (MQ=255)
                                   atcaTGGCATGTCCTTATTATGACGTGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGAt                                                                                                             <  3:162443/149‑1 (MQ=255)
                                   atcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGAt                                                                                                             >  1:314694/1‑149 (MQ=255)
                                       tGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGc                                                                                                         <  3:206792/149‑1 (MQ=255)
                                         gCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTCCCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCgg                                                                                                       >  3:98628/1‑149 (MQ=255)
                                           aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg                                                                                                     <  8:352417/149‑1 (MQ=255)
                                                cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATg                                                                                                >  6:316610/1‑149 (MQ=255)
                                                cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATg                                                                                                <  1:283306/149‑1 (MQ=255)
                                                cTTATTATGACGGGAAATGCCACCCTTTTAACATTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTAAATACAGGCAGAAACAATTGGCCTGTTCGAGTATGATCTGAGCTCGAATg                                                                                                >  8:78385/1‑149 (MQ=255)
                                                 ttattaTGACGGGAAATGCCACACTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCTATCACTTCATCACCGCGTCCGCTTATTATTTCGCGAAGAATATACAGGCTGAAACATTTg                                                                                                                              >  6:161002/1‑118 (MQ=255)
                                                   attatGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTAGATCTGCGGTGGAATGGc                                                                                              >  2:369547/1‑148 (MQ=255)
                                                      atGACGTGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTaa                                                                                           <  2:227041/148‑1 (MQ=255)
                                                      atGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTaa                                                                                           <  3:342235/148‑1 (MQ=255)
                                                         aCGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCt                                                                                                                                        <  6:309661/100‑1 (MQ=255)
                                                         aCGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCt                                                                                                                                        >  5:309661/1‑100 (MQ=255)
                                                         aCGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGATCGAGTTTGATCTGAGGGGGAATGGCTAActct                                                                                       >  6:223672/1‑149 (MQ=255)
                                                           gggAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttc                                                                                     >  2:111592/1‑149 (MQ=255)
                                                           gggAAATGCCACCCTTTTTACCTTAGCCAGTTTGGTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttc                                                                                     <  1:40754/149‑1 (MQ=255)
                                                             gAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTcttctt                                                                                   >  2:322645/1‑149 (MQ=255)
                                                               aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                                                                                 <  5:328993/149‑1 (MQ=255)
                                                               aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                                                                                 <  7:140848/149‑1 (MQ=255)
                                                               aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                                                                                 <  1:8034/149‑1 (MQ=255)
                                                                    cACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac                                                                            <  6:336344/149‑1 (MQ=255)
                                                                    cACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac                                                                            >  2:260322/1‑149 (MQ=255)
                                                                     aCCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac                                                                            >  6:132875/1‑148 (MQ=255)
                                                                       ccTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGaccac                                                                         >  7:380683/1‑149 (MQ=255)
                                                                        cTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGaccacc                                                                        <  7:289558/149‑1 (MQ=255)
                                                                            ttACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACcaca                                                                     <  5:316610/148‑1 (MQ=255)
                                                                                cTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccac                                                                <  5:132875/149‑1 (MQ=255)
                                                                                     ccAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAaca                                                           >  3:97090/1‑149 (MQ=255)
                                                                                      cAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAacac                                                          >  6:377112/1‑149 (MQ=255)
                                                                                           tgtttTCGCCAGTTCGATCACATCATCACCGCGTCCGCTGATGATTGCTCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGGGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGa                                                      <  7:381183/148‑1 (MQ=255)
                                                                                            gtttACGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGAcc                                                    >  5:297516/1‑149 (MQ=255)
                                                                                             ttttCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGa                                                                                                                    <  5:49886/84‑1 (MQ=255)
                                                                                             ttttCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGa                                                                                                                    >  6:49886/1‑84 (MQ=255)
                                                                                                tCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTTACTCTTCTTTGGCGACGACCACATCCACGATCACCGGACCGTc                                                 <  4:98628/148‑1 (MQ=255)
                                                                                                         cGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAg                                       >  2:290750/1‑149 (MQ=255)
                                                                                                               cTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGt                                 >  3:265518/1‑149 (MQ=255)
                                                                                                               cTTCATCACCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGt                                 <  5:377112/149‑1 (MQ=255)
                                                                                                                ttaatcaCAGCGTCCGCTGAAGATTGCCCGCACTATAAACATGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGATAAAACTTCTTTCGCGAACACCACATCCACCACCACCGGACCGTCCATCGAGCACGCGCGtt                                >  4:99059/4‑149 (MQ=255)
                                                                                                                   atcaCCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTg                               >  5:208527/1‑147 (MQ=255)
                                                                                                                   atcaCCGCGGCCGCGGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTATTTGGCGACCACCACATCCAACAACACCGGACCGTCGATGGAGAAGGCGCGTTGCa                             <  6:214383/149‑1 (MQ=255)
                                                                                                                     caCCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAgg                           >  2:132019/1‑149 (MQ=255)
                                                                                                                      aCCGCGTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAggg                          >  5:34635/1‑149 (MQ=255)
                                                                                                                           gTCCGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTATCTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCtt                       <  4:52028/147‑1 (MQ=255)
                                                                                                                              cGCTGATGATTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTAGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTtcatc                   <  1:369547/148‑1 (MQ=255)
                                                                                                                               gCTGAAGAATGCCCGCCGGATATACAGGCGTCAACCAATGGCCGGGTCGAGTCTGATCTGCGGTGGAATTTTTTTTTTTTATTTTTCGTCGACCACATACATCTACACCGGACCGTAGATGGAGAAGGCGCGTTGCAGGGCTTCATCaa                 <  4:316410/149‑1 (MQ=255)
                                                                                                                                 tgatgaTTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACt               >  6:390164/1‑149 (MQ=255)
                                                                                                                                   atgatTGCGCGCAGTATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACtt              >  2:253878/1‑148 (MQ=255)
                                                                                                                                     gatTGCGCGCAGGATATACAGGCTGAAACCTTTGGCCTGTTCTCGTTTGATCTGCGGGGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCa            <  5:204332/148‑1 (MQ=255)
                                                                                                                                             gcagtaTATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGGAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCtttt   >  5:375658/1‑149 (MQ=255)
                                                                                                                                              cagtaTATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACGACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCttttt  <  5:100039/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTT  >  NC_000913/909255‑909545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: