BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	753,984	G→A	A162V (GCG→GTG)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,984	0	G	A	99.0%	354.0 / NA	104	A162V (GCG→GTG)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (55/48); minor base T (0/1); total (55/49)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGA  >  NC_000913/753838‑754128

cATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAAcacg                                                                                                                                                >  8:199810/1‑149 (MQ=255)

 aTACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAAcacg                                                                                                                                                <  4:7768/148‑1 (MQ=255)

  tACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACgg                                                                                                                                               >  3:12277/1‑148 (MQ=255)

   aCGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGGAAACAAATGGCAGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGc                                                                                                                                              >  3:324948/1‑148 (MQ=255)

     ggTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAAt                                                                                                                                           >  3:263622/1‑149 (MQ=255)

       ttCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAAttt                                                                                                                                         <  7:199810/149‑1 (MQ=255)

          gCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGCCAGCAGGCGGAACACGGCAATTTCAc                                                                                                                                      >  4:268119/1‑149 (MQ=255)

           cACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACg                                                                                                                                     >  4:67308/1‑149 (MQ=255)

           cACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACg                                                                                                                                     <  2:120689/149‑1 (MQ=255)

           cACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACg                                                                                                                                     <  2:228830/149‑1 (MQ=255)

                  tGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGCCCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGa                                                                                                                              <  1:334073/149‑1 (MQ=255)

                   ggAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATCGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAg                                                                                                                             >  8:151656/1‑149 (MQ=255)

                     agaACATCATATTCAGGAAGTTACCGGCGTAGGAGAGAGCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGa                                                                                                                           <  1:268439/149‑1 (MQ=255)

                         catcatATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTgg                                                                                                                       <  5:272183/149‑2 (MQ=255)

                          atcatATTCATGAAGTTAACGGCGTAGGAGAGATCGTTTCGCGGGTAAACAAATGGCTGTCCAATGGAATACATGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGtt                                                                                                                      <  3:84020/149‑1 (MQ=255)

                          atcatATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGtt                                                                                                                      <  4:128964/149‑1 (MQ=255)

                          atcatATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGt                                                                                                                       >  7:230693/1‑148 (MQ=255)

                          atcatATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGt                                                                                                                       >  6:268702/1‑148 (MQ=255)

                          atcatATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGt                                                                                                                       >  7:204662/1‑148 (MQ=255)

                           tcatATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGCAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTa                                                                                                                     <  8:386282/149‑1 (MQ=255)

                              tatTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAGTACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGAc                                                                                                                                >  6:146169/1‑135 (MQ=255)

                              tatTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAGTACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGAc                                                                                                                                <  5:146169/135‑1 (MQ=255)

                              tatTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACa                                                                                                                  <  2:54057/149‑1 (MQ=255)

                               atTCAGGAAGTTACGGGCGTAGGACAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGCCAATTACACGGTGACGAGGATAGATAACAt                                                                                                                 >  8:216949/1‑149 (MQ=255)

                               atTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACAt                                                                                                                 >  7:161743/1‑149 (MQ=255)

                                ttCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGACGATTGTTAACATc                                                                                                                >  7:347824/1‑149 (MQ=255)

                                  cAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATc                                                                                                                >  4:270790/1‑147 (MQ=255)

                                   aGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGCCAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAg                                                                                                             >  1:348280/1‑149 (MQ=255)

                                    ggAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGc                                                                                                            >  5:227031/1‑149 (MQ=255)

                                            ccGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTaa                                                                                                                    <  3:271621/133‑1 (MQ=255)

                                            ccGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTaa                                                                                                                    >  4:271621/1‑133 (MQ=255)

                                             cGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGa                                                                                                   <  4:263622/149‑1 (MQ=255)

                                             cGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGa                                                                                                   <  8:168308/149‑1 (MQ=255)

                                                    ggAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGAAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAc                                                                                             <  5:368710/148‑1 (MQ=255)

                                                    ggAGAGATCGTTGCGCGGGTAAACAAATGGCGGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAc                                                                                             <  4:324948/148‑1 (MQ=255)

                                                     gagagaTCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAcgc                                                                                           >  7:195702/1‑149 (MQ=255)

                                                      agagaTCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAcgcc                                                                                          >  3:67727/1‑149 (MQ=255)

                                                      agagaTCGGTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCAATGGTAGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAcgcc                                                                                          <  8:130727/149‑1 (MQ=255)

                                                          aTCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAActccgc                                                                                        >  7:299716/1‑147 (MQ=255)

                                                          aTCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAcgccgc                                                                                        >  3:187486/1‑147 (MQ=255)

                                                          aTCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAcgccgc                                                                                        >  1:110844/1‑147 (MQ=255)

                                                              ttGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTc                                                                                                                                        <  7:190509/95‑1 (MQ=255)

                                                              ttGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTc                                                                                                                                        >  8:190509/1‑95 (MQ=255)

                                                              ttGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAgcgc                                                                                  >  4:63360/1‑149 (MQ=255)

                                                                 cgcgGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAgcgcgc                                                                                <  8:230693/148‑1 (MQ=255)

                                                                  gcgGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCg                                                                              <  4:365220/149‑1 (MQ=255)

                                                                  gcgGGAAAACAAAGGGCGCCCCAATGGAATACTTGT‑ACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCgg                                                                             <  3:228566/149‑1 (MQ=255)

                                                                   cgGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCgg                                                                             <  4:352792/149‑1 (MQ=255)

                                                                         aaCAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATAc                                                                       >  1:2621/1‑149 (MQ=255)

                                                                           cAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACca                                                                     <  4:67727/149‑1 (MQ=255)

                                                                           cAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACca                                                                     <  3:270790/149‑1 (MQ=255)

                                                                                 gCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACAt                                                                <  5:268702/148‑1 (MQ=255)

                                                                                   tGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGAc                                                             >  8:389882/1‑149 (MQ=255)

                                                                                   tGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGAc                                                             >  8:251033/1‑149 (MQ=255)

                                                                                     aCCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTg                                                           <  3:67308/149‑1 (MQ=255)

                                                                                          tGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCAtt                                                      >  4:314478/1‑149 (MQ=255)

                                                                                            gAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTgg                                                    <  8:161743/149‑1 (MQ=255)

                                                                                            gAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTg                                                     >  7:52418/1‑148 (MQ=255)

                                                                                                 cTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGc                                                <  7:389882/148‑1 (MQ=255)

                                                                                                 cTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGc                                                >  5:229983/1‑148 (MQ=255)

                                                                                                  ttGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGc                                                >  7:379134/1‑147 (MQ=255)

                                                                                                  ttGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGc                                                >  3:186223/1‑147 (MQ=255)

                                                                                                  ttGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGc                                                >  1:186031/1‑147 (MQ=255)

                                                                                                    gTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGt                                            >  6:149289/1‑149 (MQ=255)

                                                                                                    gTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGt                                            >  3:192306/1‑149 (MQ=255)

                                                                                                     tAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGt                                            <  4:187486/148‑1 (MQ=255)

                                                                                                       acacATCGCGGCCATGTTCGGCATTTTCGACAGCATGCGGAACAAGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTcgc                                         <  8:299716/149‑1 (MQ=255)

                                                                                                       acacATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTcgc                                         <  2:348280/149‑1 (MQ=255)

                                                                                                       acacATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTcgc                                         >  1:219183/1‑149 (MQ=255)

                                                                                                         acaTCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAAGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGa                                       >  1:173910/1‑149 (MQ=255)

                                                                                                            tCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACg                                     >  6:235655/1‑148 (MQ=255)

                                                                                                            tCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACg                                     >  8:292025/1‑148 (MQ=255)

                                                                                                               cgGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGAc                                      >  7:290893/1‑144 (MQ=255)

                                                                                                               cgGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGAc                                      <  8:290893/144‑1 (MQ=255)

                                                                                                               cgGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGaaa                                 <  8:52418/149‑1 (MQ=255)

                                                                                                               cgGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGaaa                                 <  2:173910/149‑1 (MQ=255)

                                                                                                               cgGCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGaaa                                 <  8:347824/149‑1 (MQ=255)

                                                                                                                 gCCATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAg                                >  3:23485/1‑148 (MQ=255)

                                                                                                                   cATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCAt                             >  5:394142/1‑149 (MQ=255)

                                                                                                                   cATGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCAt                             <  6:227031/149‑1 (MQ=255)

                                                                                                                     tGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATgg                           <  8:379134/149‑1 (MQ=255)

                                                                                                                     tGGTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATg                            >  7:222786/1‑148 (MQ=255)

                                                                                                                       gTCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGaa                         >  7:50170/1‑149 (MQ=255)

                                                                                                                        tCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGaa                         >  4:221383/1‑148 (MQ=255)

                                                                                                                        tCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAAc                        <  7:151656/149‑1 (MQ=255)

                                                                                                                        tCGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAAc                        <  2:186031/149‑1 (MQ=255)

                                                                                                                         cGGCATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGCCGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACa                       >  5:392400/1‑149 (MQ=255)

                                                                                                                            cATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGAc                    <  3:63360/149‑1 (MQ=255)

                                                                                                                            cATTTTCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGAc                    <  2:288081/149‑1 (MQ=255)

                                                                                                                              ttttCGACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACgg                  <  1:378712/149‑1 (MQ=255)

                                                                                                                               tttGGACAGCAGGCGGAAGTCGGCAATTTCACGGTGAAGTGGATTTTTAACATCCAGCTAGTCTTGATAGAACGCCGCCAGCGCGCCTTTAATACCTCAGATGAATGACATTGGATGCGAGTAGAGTCGGAATGCATGGAACAGAAgg                  <  8:204662/148‑1 (MQ=255)

                                                                                                                                   gACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAAt             <  5:47471/149‑1 (MQ=255)

                                                                                                                                   gACAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAAt             <  6:392400/149‑1 (MQ=255)

                                                                                                                                    aCAGCAGGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATc            <  3:221383/149‑1 (MQ=255)

                                                                                                                                       gcagGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGc         >  5:282653/1‑149 (MQ=255)

                                                                                                                                        cagGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCt        >  4:241079/1‑149 (MQ=255)

                                                                                                                                        cagGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCt        >  6:114382/1‑149 (MQ=255)

                                                                                                                                         agGCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTc       <  1:63399/149‑1 (MQ=255)

                                                                                                                                          ggCGGAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCg      >  1:389426/1‑149 (MQ=255)

                                                                                                                                              gAACACGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAAGGCCGCCAGCGCGCCGGTAATACCACACCTGACTGCCATTGGATGCGAGTCGCGACGGCAAGCATGGACCAGACGGGTAATCTGCTCGTGGa  >  6:369580/1‑149 (MQ=255)

CATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCGTAGGAGAGATCGTTGCGCGGGTAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGA  >  NC_000913/753838‑754128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`