Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 168,474 C→T Q331* (CAG→TAG)  fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913168,4740CT98.1% 351.5 / ‑2.3 103Q331* (CAG→TAG) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (51/50);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 103 total aligned reads displayed.

AGAGGGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGT  >  NC_000913/168329‑168619
                                                                                                                                                 |                                                                                                                                                  
agagGGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAg                                                                                                                                                  >  8:143978/1‑148 (MQ=255)
 gagGGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGaa                                                                                                                                                <  3:96425/149‑1 (MQ=255)
  agGGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAAc                                                                                                                                               <  8:353004/149‑1 (MQ=255)
  agGGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAAc                                                                                                                                               >  7:9277/1‑149 (MQ=255)
                ccGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTg                                                                                                                                  >  4:144825/1‑148 (MQ=255)
                   cTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGa                                                                                                                                 <  7:296390/146‑1 (MQ=255)
                   cTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGa                                                                                                                                 >  8:296390/1‑146 (MQ=255)
                    tGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTgcgc                                                                                                                                         <  4:56486/137‑1 (MQ=255)
                      ccGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGaaaaca                                                                                                                            >  4:329279/1‑148 (MQ=255)
                        gAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGaaaacaaaa                                                                                                                         <  2:148229/149‑1 (MQ=255)
                          aCGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAAcc                                                                                                                       <  3:254338/149‑1 (MQ=255)
                            ggTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTc                                                                                                                     <  5:240115/149‑1 (MQ=255)
                                 gCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGc                                                                                                                   <  1:329116/146‑1 (MQ=255)
                                 gCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGc                                                                                                                   >  2:329116/1‑146 (MQ=255)
                                      tGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGc                                                                                                           >  8:321687/1‑149 (MQ=255)
                                        ccGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGt                                                                                                         <  2:275847/149‑1 (MQ=255)
                                         cGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGtt                                                                                                        >  2:16908/1‑149 (MQ=255)
                                         cGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGtt                                                                                                        <  2:7490/149‑1 (MQ=255)
                                           aCAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTa                                                                                                      <  7:354495/149‑1 (MQ=255)
                                           aCAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTa                                                                                                      <  5:217945/149‑1 (MQ=255)
                                           aCAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTa                                                                                                      >  4:189511/1‑149 (MQ=255)
                                             aGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATg                                                                                                    <  3:329279/149‑1 (MQ=255)
                                             aGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGGGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATg                                                                                                    <  6:62655/149‑1 (MQ=255)
                                                cTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTAGTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGtt                                                                                                 <  1:243844/149‑1 (MQ=255)
                                                   tAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTa                                                                                                <  5:10547/147‑1 (MQ=255)
                                                    aaTGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTATATGGTTACgg                                                                                             >  1:391685/1‑149 (MQ=255)
                                                     aTGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTAt                                                                                                     <  6:88872/140‑1 (MQ=255)
                                                     aTGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTAt                                                                                                     >  5:88872/1‑140 (MQ=255)
                                                        aaGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGt                                                                                          <  6:34102/148‑1 (MQ=255)
                                                        aaGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTc                                                                                         <  3:144825/149‑1 (MQ=255)
                                                        aaGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTc                                                                                         <  7:187411/149‑1 (MQ=255)
                                                            ggCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGAGCACGAAATTAACGACACCTATAATCTGCCTTAGAACCTGCGCTTTGCTTAAAACAAAACCTCTCAAAACAGCGAATATGGTTACTGCGtcttct                                                                                     >  5:227637/1‑149 (MQ=255)
                                                             gCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTc                                                                                    <  4:327730/149‑1 (MQ=255)
                                                             gCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTc                                                                                    <  1:79559/149‑1 (MQ=255)
                                                             gCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTc                                                                                    >  4:113137/1‑149 (MQ=255)
                                                             gCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCGTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTc                                                                                    <  7:389311/149‑1 (MQ=255)
                                                                  gaaCCACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATc                                                                               <  6:176825/149‑1 (MQ=255)
                                                                       gaccatcttCTCATTACGAGCAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACCCCATTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAACCCTCGCAAAGCAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGc                                                                           >  3:177468/8‑148 (MQ=255)
                                                                        cacCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTcc                                                                                   >  1:327083/1‑139 (MQ=255)
                                                                        cacCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTcc                                                                                   <  2:327083/139‑1 (MQ=255)
                                                                         acCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGaa                                                                        <  3:24090/149‑1 (MQ=255)
                                                                         acCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGaa                                                                        >  6:91442/1‑149 (MQ=255)
                                                                           cTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATg                                                                      >  7:404456/1‑149 (MQ=255)
                                                                           cTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATg                                                                      <  3:174946/149‑1 (MQ=255)
                                                                           cTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGATGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATg                                                                      >  5:268279/1‑149 (MQ=255)
                                                                              ttCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCt                                                                    >  1:274388/1‑148 (MQ=255)
                                                                                ctcGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTa                                                                  <  8:216477/148‑1 (MQ=255)
                                                                                 tcGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTa                                                                  >  7:372489/1‑147 (MQ=255)
                                                                                 tcGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTa                                                                  >  8:49774/1‑147 (MQ=255)
                                                                                   gTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGc                                                                           <  5:248367/136‑1 (MQ=255)
                                                                                   gTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACa                                                                <  8:404456/147‑1 (MQ=255)
                                                                                   gTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACAACTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTAc                                                                 <  6:28266/146‑1 (MQ=255)
                                                                                    tAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGc                                                              >  8:320646/1‑148 (MQ=255)
                                                                                     aaTGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCa                                                             >  5:201895/1‑148 (MQ=255)
                                                                                         agaagaTGGTCGGCTACAGCTTCGATCACGAATTTAACGCCACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAg                                                        >  2:311024/1‑149 (MQ=255)
                                                                                         agaagaTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAg                                                        >  2:354599/1‑149 (MQ=255)
                                                                                          gaagaTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAgt                                                       <  1:16908/149‑1 (MQ=255)
                                                                                            agaTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAgtgt                                                     <  7:320646/149‑1 (MQ=255)
                                                                                             gaTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGtgtg                                                    >  8:164225/1‑149 (MQ=255)
                                                                                              aTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCTCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGc                                                  >  3:130739/1‑149 (MQ=255)
                                                                                              aTGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGc                                                  <  7:164225/149‑1 (MQ=255)
                                                                                                ggTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCgg                                                >  5:181248/1‑149 (MQ=255)
                                                                                                 gTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCgg                                                >  3:90672/1‑148 (MQ=255)
                                                                                                     gCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTa                                           <  8:9277/149‑1 (MQ=255)
                                                                                                      cTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAg                                          >  5:166994/1‑149 (MQ=255)
                                                                                                       tACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTc                                                                                                                     <  3:40358/74‑1 (MQ=255)
                                                                                                       tACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTc                                                                                                                     >  4:40358/1‑74 (MQ=255)
                                                                                                         cAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTATATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAgc                                         <  2:391685/147‑1 (MQ=255)
                                                                                                            cTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAAc                                                          <  3:83896/128‑1 (MQ=255)
                                                                                                            cTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAAc                                                          >  4:83896/1‑128 (MQ=255)
                                                                                                            cTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAg                                    >  1:48932/1‑149 (MQ=255)
                                                                                                            cTTCGATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAg                                    >  3:80447/1‑149 (MQ=255)
                                                                                                                gATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGc                                               >  8:256276/1‑134 (MQ=255)
                                                                                                                gATCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGc                                               <  7:256276/134‑1 (MQ=255)
                                                                                                                 aTCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGAt                               <  6:181248/149‑1 (MQ=255)
                                                                                                                 aTCACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTATGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGa                                >  2:133179/1‑148 (MQ=255)
                                                                                                                 aTCACGAATTTAACGACACCTTTACTGAGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTCGCGCCAGCGGa                                >  8:362774/1‑148 (MQ=255)
                                                                                                                   cACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTAc                                                                 >  4:31770/1‑114 (MQ=255)
                                                                                                                   cACGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTAc                                                                 <  3:31770/114‑1 (MQ=255)
                                                                                                                    aCGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATa                              >  6:386039/1‑147 (MQ=255)
                                                                                                                     cGAATTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTAAACAAACCATTTGCCGGCTTTGCCGCAAGCGGATAAAg                           >  1:105510/1‑149 (MQ=255)
                                                                                                                       aaTTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGc                         <  4:80447/149‑1 (MQ=255)
                                                                                                                        aTTTAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGcc                        <  3:113137/149‑1 (MQ=255)
                                                                                                                         tttAACGACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCa                       <  7:49774/149‑1 (MQ=255)
                                                                                                                               gACACCTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCt                 <  7:321687/149‑1 (MQ=255)
                                                                                                                                    cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTa                                                                                                      >  4:385407/1‑60 (MQ=255)
                                                                                                                                    cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTa                                                                                                      <  3:385407/60‑1 (MQ=255)
                                                                                                                                    cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCACACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCAGTATCTGGCAc            >  2:345133/1‑149 (MQ=255)
                                                                                                                                    cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCAc            >  6:129237/1‑149 (MQ=255)
                                                                                                                                    cTTTACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCACCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCAc            >  7:32214/1‑149 (MQ=255)
                                                                                                                                     tttACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCt                 <  5:111286/143‑1 (MQ=255)
                                                                                                                                     tttACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCt                 >  6:111286/1‑143 (MQ=255)
                                                                                                                                     tttACTGTGCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACg           >  1:258695/1‑149 (MQ=255)
                                                                                                                                          tgtgCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTaaa       >  3:162479/1‑148 (MQ=255)
                                                                                                                                          tgtgCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTaaa       >  7:59536/1‑148 (MQ=255)
                                                                                                                                          tgtgCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTaaa       >  7:51070/1‑148 (MQ=255)
                                                                                                                                          tgtgCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTaaa       >  1:42316/1‑148 (MQ=255)
                                                                                                                                            tgCGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAAt      <  4:162479/147‑1 (MQ=255)
                                                                                                                                              cGTTAGAACCTGCGCTTTGCTTTAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATAcg   <  4:130739/148‑1 (MQ=255)
                                                                                                                                              cGTTAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATAcgt  <  5:386039/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGAGGGAACCGTTGAGCCGCTGCCGAACGGTAAGCGTCTGCCGACAGACTTTAATGAAGGGGCGAAGAACAACACCTATTCTCGTAATGAGAAGATGGTCGGCTACAGCTTCGATCACGAATTTAACGACACCTTTACTGTGCGTCAGAACCTGCGCTTTGCTGAAAACAAAACCTCGCAAAACAGCGTTTATGGTTACGGCGTCTGCTCCGATCCGGCGAATGCTTACAGCAAACAGTGTGCGGCATTAGCGCCAGCGGATAAAGGCCATTATCTGGCACGTAAATACGT  >  NC_000913/168329‑168619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: