breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 9,280 | G→A | R750H (CGT→CAT) | gyrA → | DNA topoisomerase (ATP‑hydrolyzing) subunit A |
JC | 62,818 | +CAATTT | intergenic (+83/+228) | USA300HOU_0056 → / ← speG | hypothetical protein/diamine N‑acetyltransferase |
RA | 774,414 | G→A | G555D (GGT→GAT) | fruA → | PTS family fructose/mannitol (fru) porter component IIABC |
RA | 1,416,458 | A→T | I216N (ATT→AAT) | oppD ← | oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein |
RA | 1,513,930 | A→T | L413F (TTA→TTT) | USA300HOU_1379 → | hypothetical membrane protein |
RA | 1,646,189 | G→C | A155G (GCA→GGA) | pepP ← | M24B family Xaa‑Pro dipeptidase |
RA | 2,010,363 | C→T | C146Y (TGT→TAT) | ygaD ← | ABC superfamily ATP binding cassette transporter, membrane protein |
RA | 2,026,955 | G→A | T331I (ACA→ATA) | vraS ← | sensor histidine kinase VraS |
RA | 2,279,705 | G→A | intergenic (+1373/+6659) | mtlD → / ← glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
RA | 2,319,519 | G→A | Q152* (CAA→TAA) | asp23 ← | alkaline shock protein 23 |
RA | 2,373,558 | A→T | M73K (ATG→AAG) | pbuG ← | NCS2 family nucleobase:cation symporter‑2 |
RA | 2,532,298 | A→T | T15T (ACA→ACT) | murN → | FemAB family peptidoglycan biosynthesis protein |
RA | 2,785,994 | C→A | intergenic (‑207/+217) | aur ← / ← isaB | M04 family aureolysin/immunodominant antigen B |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | CP000730 | 58687 | 58992 | 306 | 7 [5] | [6] 7 | USA300HOU_0049/USA300HOU_0050 | hypothetical protein/hypothetical protein |
* | * | ÷ | CP000730 | 909106 | 909241 | 136 | 8 [6] | [6] 7 | ear/USA300HOU_0868 | Ear protein/hypothetical protein |
* | * | ÷ | CP000730 | 983129 | 983247 | 119 | 7 [5] | [6] 8 | USA300HOU_0938 | hypothetical membrane protein |
* | * | ÷ | CP000730 | 1608852 | 1609038 | 187 | 9 [6] | [6] 7 | [USA300HOU_1492] | [USA300HOU_1492] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP000730 | 512620 = | 29 (1.000) | 24 (1.190) +40 bp |
22/184 | 0.1 | 54.3% | intergenic (+688/‑173) | recR/rrs1 | recombination protein RecR/16S ribosomal RNA |
? | CP000730 | 1998278 = | NA (NA) | intergenic (‑6/+5) | USA300HOU_t0050/rrf4 | tRNA‑Val/5S ribosomal RNA |