breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 2,246 C→A 9.5% S637Y (TCT→TAT)  thrA → Bifunctional aspartokinase/homoserine dehydrogenase 1
RA 14,995 G→T 5.4% L276L (CTG→CTT dnaJ → chaperone Hsp40, DnaK co‑chaperone
RA 37,858 G→T 5.9% intergenic (‑34/+40) caiC ← / ← caiB putative crotonobetaine/carnitine‑CoA ligase/crotonobetainyl CoA:carnitine CoA transferase
RA 56,580 G→T 6.6% S177Y (TCT→TAT)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component
RA 72,085 C→A 5.2% intergenic (‑118/+46) thiB ← / ← sgrR thiamine‑, thiamine pyrophosphate‑, thiamine monophosphate‑binding ABC transporter periplasmic protein; ThiBPQ thiamine, thiamine pyrophosphate importer/transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 81,818 A→G 8.7% intergenic (+19/‑299) leuO → / → ilvI global transcription factor/acetolactate synthase 3 large subunit
RA 85,369 G→A 5.3% P285P (CCG→CCA cra → transcriptional repressor‑activator for carbon metabolism
RA 103,901 C→A 7.0% T286T (ACC→ACA lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
RA 117,751 G→T 6.0% G96G (GGC→GGA aroP ← aromatic amino acid transporter
RA 121,793 G→T 5.8% E764* (GAG→TAG)  aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
RA 143,414 G→T 6.1% intergenic (‑233/‑41) panD ← / → yadD aspartate 1‑decarboxylase/transposase_31 family protein
RA 158,750 G→T 6.6% L53L (CTG→CTT hrpB → putative ATP‑dependent helicase
RA 181,751 C→A 7.0% P228P (CCG→CCT dapD ← 2,3,4,5‑tetrahydropyridine‑2‑carboxylate N‑succinyltransferase
RA 215,091 G→T 5.9% R58S (CGT→AGT)  proS ← prolyl‑tRNA synthetase
RA 221,333 T→C 6.5% noncoding (1076/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,338 A→G 5.8% noncoding (1081/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,373:1 +G 7.0% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 7.2% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 6.9% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 6.9% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 7.1% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 7.1% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 7.4% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 7.8% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 8.3% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 8.8% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 9.6% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 9.5% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 13.0% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 12.9% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 12.1% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 246,847 G→T 6.9% pseudogene (289/756 nt) lafU → pseudogene, lateral flagellar motor protein fragment
RA 246,848 G→T 10.3% pseudogene (290/756 nt) lafU → pseudogene, lateral flagellar motor protein fragment
RA 249,874 G→C 7.0% intergenic (+226/‑92) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 249,908 A→G 9.6% intergenic (+260/‑58) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 261,370 C→A 7.5% E143D (GAG→GAT ykfB ← CP4‑6 prophage; uncharacterized protein
RA 263,285 G→A 5.3% D144D (GAC→GAT yafZ ← CP4‑6 prophage; conserved protein
RA 272,792 G→T 6.3% G123W (GGG→TGG)  mmuM → CP4‑6 prophage; S‑methylmethionine:homocysteine methyltransferase
RA 274,938 T→A 14.7% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 334,782 G→T 10.1% T249T (ACG→ACT yahG → DUF1116 family protein
RA 357,678 G→T 5.6% P405T (CCG→ACG)  lacY ← lactose permease
RA 370,556:1 +C 6.6% intergenic (+219/‑359) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,557 T→A 6.6% intergenic (+220/‑358) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,558 T→A 6.4% intergenic (+221/‑357) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 371,378 C→T 5.1% A155V (GCG→GTG)  mhpT → 3‑hydroxyphenylpropionic transporter
RA 372,807 Δ1 bp 7.6% intergenic (+40/+184) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,808 G→A 7.6% intergenic (+41/+183) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 383,233 C→A 7.4% P269P (CCC→CCA tauC → taurine transporter subunit
RA 383,649 G→T 8.7% A133A (GCG→GCT tauD → taurine dioxygenase, 2‑oxoglutarate‑dependent
RA 384,096 G→T 5.9% A282A (GCG→GCT tauD → taurine dioxygenase, 2‑oxoglutarate‑dependent
RA 404,674 G→T 8.4% R268S (CGT→AGT)  rdgC ← nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
RA 410,144 C→A 6.1% D356Y (GAC→TAC)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 422,038 C→A 6.0% P149H (CCT→CAT)  tgt → tRNA‑guanine transglycosylase
RA 502,079 C→A 11.9% D154Y (GAT→TAT)  ybaK ← Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase
RA 510,340 T→C 6.1% D297G (GAC→GGC)  qmcA ← PHB domain membrane‑anchored putative protease
RA 519,279 G→T 6.9% W188L (TGG→TTG)  rhsD → Rhs family putative polymorphic toxin
RA 551,543 C→A 6.9% W156L (TGG→TTG)  ybcI ← DUF457 family inner membrane protein
RA 551,682 C→A 7.5% G110C (GGT→TGT)  ybcI ← DUF457 family inner membrane protein
RA 552,558 C→A 7.0% G214C (GGC→TGC)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/ 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 573,883 G→C 5.6% T107T (ACG→ACC
R34P (CGG→CCG) 
rzpD →
rzoD →
DLP12 prophage; putative murein endopeptidase
DLP12 prophage; putative lipoprotein
RA 584,340 G→T 5.5% Q691K (CAG→AAG)  nfrA ← bacteriophage N4 receptor, outer membrane subunit
RA 592,235 C→A 6.0% Q394K (CAG→AAG)  cusC → copper/silver efflux system, outer membrane component
RA 611,322 C→A 7.4% P570P (CCC→CCA entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 628,822 C→A 7.8% V38L (GTG→TTG)  ybdH ← putative oxidoreductase
RA 661,028 C→A 6.3% G248C (GGC→TGC)  mrdB ← cell wall shape‑determining protein
RA 676,071 A→G 7.3% E135G (GAG→GGG)  djlC → J domain‑containing HscC co‑chaperone; Hsc56
RA 681,730 G→T 6.2% A132A (GCC→GCA gltJ ← glutamate, aspartate ABC transporter permease subunit
RA 695,621 G→T 6.1% N54K (AAC→AAA umpH ← UMP phosphatase
RA 695,660 C→A 5.9% L41L (CTG→CTT umpH ← UMP phosphatase
RA 695,741 C→A 7.3% L14L (CTG→CTT umpH ← UMP phosphatase
RA 706,553 C→A 8.9% A123A (GCG→GCT fldA ← flavodoxin 1
RA 709,459 C→A 6.2% P149Q (CCG→CAG)  pgm → phosphoglucomutase
RA 720,256 G→T 6.0% T60T (ACC→ACA kdpC ← potassium translocating ATPase, subunit C
RA 726,100 A→G 6.5% R354R (CGA→CGG rhsC → Rhs family putative polymorphic toxin
RA 732,050 C→T 5.3% pseudogene (150/240 nt) ybfQ → pseudogene, H repeat‑associated protein
RA 758,706 G→T 8.7% K79N (AAG→AAT sucC → succinyl‑CoA synthetase, beta subunit
RA 762,555 C→A 6.1% A372A (GCC→GCA mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
RA 769,751 C→A 5.0% Q34K (CAA→AAA)  cydX → cytochrome d (bd‑I) ubiquinol oxidase subunit X
RA 778,047 G→T 6.0% L169F (TTG→TTT nadA → quinolinate synthase, subunit A
RA 778,536 G→T 5.4% L332L (CTG→CTT nadA → quinolinate synthase, subunit A
RA 797,839 C→A 5.1% S166Y (TCC→TAC)  ybhI → putative transporter
RA 809,978 G→T 10.0% G333W (GGG→TGG)  uvrB → excinulease of nucleotide excision repair, DNA damage recognition component
RA 815,555 G→A 8.1% V72V (GTG→GTA ybhL → UPF0005 family inner membrane protein
RA 820,118 G→T 9.0% T11T (ACG→ACT ybhQ → inner membrane protein
RA 827,767 G→T 9.4% intergenic (+75/+157) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein
RA 829,188 G→T 12.6% V221V (GTG→GTT dinG → ATP‑dependent DNA helicase
RA 836,788 G→T 6.1% P67Q (CCG→CAG)  fiu ← catecholate siderophore receptor Fiu
RA 842,571 T→C 6.0% Q2R (CAG→CGG)  glnP ← glutamine transporter subunit
RA 845,543 G→T 7.3% S4* (TCA→TAA)  rhtA ← threonine and homoserine efflux system
RA 871,951 G→T 9.0% S387S (TCG→TCT yliF → putative membrane‑anchored diguanylate cyclase
RA 879,433 C→T 7.0% A102V (GCG→GTG)  mdfA → multidrug efflux system protein
RA 898,384 C→A 5.8% E21* (GAA→TAA)  artI ← arginine transporter subunit
RA 913,805 G→T 7.3% L258M (CTG→ATG)  ybjX ← DUF535 family protein
RA 918,948 G→T 6.2% D77Y (GAC→TAC)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 922,710 G→T 7.3% S60* (TCG→TAG)  aat ← leucyl/phenylalanyl‑tRNA‑protein transferase
RA 927,060 A→G 7.4% V149V (GTT→GTC trxB ← thioredoxin reductase, FAD/NAD(P)‑binding
RA 954,950 G→T 5.9% G228V (GGG→GTG)  aroA → 5‑enolpyruvylshikimate‑3‑phosphate synthetase
RA 967,444 G→T 7.8% M79I (ATG→ATT ycbJ → protein kinase‑like domain protein
RA 969,800 C→A 6.3% P9H (CCC→CAC)  mukF → chromosome condensin MukBEF, kleisin‑like subunit, binds calcium
RA 987,444 C→A 7.5% P456Q (CCG→CAG)  pepN → aminopeptidase N
RA 1,012,559 C→A 13.3% A399A (GCG→GCT ycbZ ← putative peptidase
RA 1,018,497 C→A 5.0% Q281H (CAG→CAT yccS ← putative transporter, FUSC superfamily inner membrane protein, tandem domains
RA 1,027,508 G→T 6.6% intergenic (‑340/‑87) serT ← / → hyaA tRNA‑Ser/hydrogenase 1, small subunit
RA 1,037,805 G→T 7.6% P621Q (CCG→CAG)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation
RA 1,045,283 T→G 6.7% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,063,690 C→A 10.7% H52N (CAC→AAC)  ymdF → KGG family protein
RA 1,069,156 G→T 6.8% A104A (GCC→GCA rutA ← pyrimidine oxygenase, FMN‑dependent
RA 1,069,333 G→T 8.9% T45T (ACC→ACA rutA ← pyrimidine oxygenase, FMN‑dependent
RA 1,120,563 C→A 6.6% G74C (GGT→TGT)  mdtH ← multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
RA 1,121,695 C→A 7.7% P28Q (CCG→CAG)  yceH → UPF0502 family protein
RA 1,124,505 C→A 11.9% P404Q (CCG→CAG)  murJ → putative peptidoglycan lipid II flippase
RA 1,132,561 G→T 6.2% G278W (GGG→TGG)  flgI → putative flagellar basal body protein
RA 1,132,590 G→T 6.6% Q287H (CAG→CAT flgI → putative flagellar basal body protein
RA 1,149,849 C→A 6.3% T94T (ACC→ACA yceG → septation protein, ampicillin sensitivity
RA 1,161,440 C→A 7.5% intergenic (+299/‑101) ycfP → / → ndh putative UPF0227 family esterase/respiratory NADH dehydrogenase 2/cupric reductase
RA 1,165,168 C→A 11.4% R221R (CGG→CGT ldtC ← L,D‑transpeptidase linking Lpp to murein
RA 1,166,355 C→A 20.1% T1022T (ACG→ACT mfd ← transcription‑repair coupling factor
RA 1,182,767 G→A 8.2% R310R (CGC→CGT roxA ← 50S ribosomal protein L16 arginine hydroxylase; 2‑oxoglutarate oxygenase
RA 1,187,052 G→T 9.1% H131N (CAC→AAC)  purB ← adenylosuccinate lyase
RA 1,201,592 G→T 6.4% pseudogene (406/412 nt) beeE → pseudogene, portal protein family, e14 prophage;Phage or Prophage Related
RA 1,214,003 G→A 6.9% G407D (GGC→GAC)  ycgG → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 1,244,752 C→A 14.3% A154A (GCG→GCT dhaL ← dihydroxyacetone kinase, C‑terminal domain
RA 1,261,188 G→T 8.7% D241Y (GAT→TAT)  prfA → peptide chain release factor RF‑1
RA 1,269,438 G→T 6.0% G67C (GGC→TGC)  ychO → putative invasin
RA 1,277,694 G→T 7.6% W792L (TGG→TTG)  narG → nitrate reductase 1, alpha subunit
RA 1,299,192 G→A 5.4% G61D (GGT→GAT)  oppD → oligopeptide transporter subunit
RA 1,299,622 G→T 7.4% Q204H (CAG→CAT oppD → oligopeptide transporter subunit
RA 1,307,375 C→A 7.0% L182L (CTG→CTT yciC ← UPF0259 family inner membrane protein
RA 1,324,432 G→T 6.1% G282* (GGA→TGA)  sohB → inner membrane protein, S49 peptidase family protein
RA 1,326,555 G→T 8.7% A417A (GCG→GCT topA → DNA topoisomerase I, omega subunit
RA 1,330,250 G→T 8.5% G55C (GGT→TGT)  acnA → aconitate hydratase 1
RA 1,335,780 G→T 9.6% M322I (ATG→ATT yciM → envelope integrity maintenance protein; EnvC‑interacting protein
RA 1,348,900 G→T 5.1% I276I (ATC→ATA sapB ← antimicrobial peptide transport ABC transporter permease
RA 1,354,800 C→A 8.6% L122L (CTG→CTT puuA ← glutamate‑‑putrescine ligase
RA 1,369,097 T→C 7.0% S251P (TCA→CCA)  ycjP → inner membrane putative ABC superfamily sugar transporter permease
RA 1,369,326 C→A 9.5% P36Q (CCG→CAG)  ycjQ → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,369,348 C→A 9.9% V43V (GTC→GTA ycjQ → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,373,959 G→T 6.2% D607Y (GAC→TAC)  ycjT → putative family 65 glycosyl hydrolase
RA 1,410,806 C→A 8.1% D280Y (GAC→TAC)  recE ← Rac prophage; exonuclease VIII, 5' ‑> 3' specific dsDNA exonuclease
RA 1,422,040 A→G 12.2% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 14.1% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,168 A→C 5.2% pseudogene (816/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 5.3% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 8.0% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,798 A→G 5.8% pseudogene (186/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,426,176 A→G 5.3% K957K (AAA→AAG stfR → Rac prophage; putative tail fiber protein
RA 1,426,179 T→C 5.3% P958P (CCT→CCC stfR → Rac prophage; putative tail fiber protein
RA 1,434,929 G→T 5.4% A38D (GCC→GAC)  pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,460,458 T→C 12.0% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,461,046 C→T 5.6% pseudogene (1398/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,461,055 A→G 5.9% pseudogene (1407/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,469,361 G→T 7.2% intergenic (+23/‑40) ydbC → / → ydbD putative NAD(P)‑binding oxidoreductase/PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,477,405 C→A 7.3% R30S (CGT→AGT)  hrpA → putative ATP‑dependent helicase
RA 1,478,426 G→A 6.9% R370H (CGT→CAT)  hrpA → putative ATP‑dependent helicase
RA 1,480,624 G→T 5.5% G1103C (GGC→TGC)  hrpA → putative ATP‑dependent helicase
RA 1,502,480 G→A 7.7% A481A (GCG→GCA ydcP → putative peptidase
RA 1,510,188 G→T 6.4% L118L (CTG→CTT patD → gamma‑aminobutyraldehyde dehydrogenase
RA 1,510,257 C→A 6.6% V141V (GTC→GTA patD → gamma‑aminobutyraldehyde dehydrogenase
RA 1,515,195 C→A 9.1% intergenic (+11/+25) mcbR → / ← yncD colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled/putative iron outer membrane transporter
RA 1,529,104 C→T 7.4% T275M (ACG→ATG)  nhoA → N‑hydroxyarylamine O‑acetyltransferase
RA 1,537,351 A→G 7.5% F323L (TTT→CTT)  narU ← nitrate/nitrite transporter
RA 1,544,248 C→A 7.8% P864Q (CCG→CAG)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,567,063 C→A 7.3% Q799H (CAG→CAT pqqL ← putative periplasmic M16 family zinc metalloendopeptidase
RA 1,593,534 G→T 8.2% S311R (AGC→AGA lsrK ← autoinducer‑2 (AI‑2) kinase
RA 1,605,623 C→A 11.1% A163A (GCG→GCT yneF ← putative membrane‑bound diguanylate cyclase
RA 1,609,146 C→A 5.9% P29Q (CCG→CAG)  yneJ → putative DNA‑binding transcriptional regulator
RA 1,623,684 A→G 6.7% intergenic (+9/+26) ydfZ → / ← ydfI selenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase
RA 1,639,861 C→A 6.6% R10R (CGG→CGT relE ← Qin prophage; toxin of the RelE‑RelB toxin‑antitoxin system
RA 1,653,563 C→A 9.3% S413* (TCG→TAG)  ynfE → putative selenate reductase, periplasmic
RA 1,654,470 G→T 6.3% E715D (GAG→GAT ynfE → putative selenate reductase, periplasmic
RA 1,660,129 G→T 7.7% V186V (GTG→GTT clcB → H(+)/Cl(‑) exchange transporter
RA 1,663,175 C→A 14.2% E225D (GAG→GAT ynfL ← LysR family putative transcriptional regulator
RA 1,668,970 A→C 7.6% G267G (GGA→GGC tqsA → pheromone AI‑2 transporter
RA 1,680,075 C→T 6.2% P257P (CCG→CCA fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,688,636 C→A 6.8% G565C (GGT→TGT)  uidA ← beta‑D‑glucuronidase
RA 1,702,975 G→A 5.9% A600A (GCG→GCA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,712,312 C→A 6.4% intergenic (‑48/+11) pdxH ← / ← mliC pyridoxine 5'‑phosphate oxidase/inhibitor of c‑type lysozyme, membrane‑bound; putative lipoprotein
RA 1,721,018 G→T 7.7% G35C (GGT→TGT)  nemA → chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
RA 1,738,293 G→T 6.9% G194C (GGC→TGC)  mdtK → multidrug efflux system transporter
RA 1,739,658 G→A 5.9% H243Y (CAT→TAT)  ydhQ ← autotransporter adhesin‑related protein
RA 1,745,816 C→A 5.3% G56W (GGG→TGG)  ydhW ← FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
RA 1,758,455 C→A 7.7% P63P (CCG→CCT sufA ← Fe‑S cluster assembly protein
RA 1,762,599 G→T 10.9% P115H (CCT→CAT)  ydiJ ← putative FAD‑linked oxidoreductase
RA 1,766,789 C→A 7.7% F7L (TTC→TTA ydiN → MFS transporter superfamily protein
RA 1,803,884 C→T 5.6% A83V (GCC→GTC)  yniC → 2‑deoxyglucose‑6‑P phosphatase
RA 1,806,192 C→A 5.3% A334A (GCC→GCA ydjN → putative transporter
RA 1,822,360 C→A 17.0% A53S (GCC→TCC)  astB ← succinylarginine dihydrolase
RA 1,823,523 G→T 6.1% P157T (CCG→ACG)  astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,823,904 G→T 8.7% Q30K (CAA→AAA)  astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,842,774 C→A 12.3% G54W (GGG→TGG)  ydjA ← putative oxidoreductase
RA 1,855,573 C→A 8.5% R6L (CGG→CTG)  ydjL ← putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,881,465 C→A 6.4% G262C (GGT→TGT)  rnd ← ribonuclease D
RA 1,926,644 C→A 9.5% G124C (GGT→TGT)  eda ← multifunctional 2‑keto‑3‑deoxygluconate 6‑phosphate aldolase and 2‑keto‑4‑hydroxyglutarate aldolase and oxaloacetate decarboxylase
RA 1,926,807 C→A 6.8% V69V (GTG→GTT eda ← multifunctional 2‑keto‑3‑deoxygluconate 6‑phosphate aldolase and 2‑keto‑4‑hydroxyglutarate aldolase and oxaloacetate decarboxylase
RA 1,928,510 C→A 5.7% G118W (GGG→TGG)  edd ← 6‑phosphogluconate dehydratase
RA 1,928,878 G→T 7.0% intergenic (‑17/+218) edd ← / ← zwf 6‑phosphogluconate dehydratase/glucose‑6‑phosphate 1‑dehydrogenase
RA 1,931,677 C→T 10.4% R257* (CGA→TGA)  yebK → putative DNA‑binding transcriptional regulator
RA 1,960,152 C→A 6.0% V99V (GTG→GTT flhB ← flagellin export apparatus, substrate specificity protein
RA 1,961,356 G→T 5.6% A113A (GCC→GCA cheY ← chemotaxis regulator transmitting signal to flagellar motor component
RA 1,965,985 G→T 8.3% R322S (CGC→AGC)  tar ← methyl‑accepting chemotaxis protein II
RA 1,966,679 G→T 8.3% L90L (CTC→CTA tar ← methyl‑accepting chemotaxis protein II
RA 1,967,560 C→A 9.5% E13* (GAG→TAG)  cheW ← purine‑binding chemotaxis protein
RA 1,974,819 C→A 9.0% R92L (CGG→CTG)  otsA ← trehalose‑6‑phosphate synthase
RA 1,980,633 C→A 5.3% P76P (CCC→CCA ftnB → ferritin B, putative ferrous iron reservoir
RA 1,992,317 C→A 6.0% L215L (CTG→CTT dcyD ← D‑cysteine desulfhydrase, PLP‑dependent
RA 2,002,998 G→T 6.2% V13V (GTG→GTT yedF → putative TusA family sulfurtransferase
RA 2,018,276 T→C 6.0% K10R (AAA→AGA)  dsrB ← uncharacterized protein
RA 2,018,279 A→T 5.8% V9D (GTC→GAC)  dsrB ← uncharacterized protein
RA 2,018,282 G→C 5.9% T8R (ACA→AGA)  dsrB ← uncharacterized protein
RA 2,108,391 C→A 7.2% S329S (TCG→TCT wcaM ← colanic acid biosynthesis protein
RA 2,113,263 G→T 5.8% P126H (CCC→CAC)  wzxC ← putative colanic acid exporter
RA 2,116,924 G→T 7.0% R360S (CGC→AGC)  cpsB ← mannose‑1‑phosphate guanyltransferase
RA 2,137,201 C→A 5.4% P152H (CCT→CAT)  yegE → putative diguanylate cyclase
RA 2,189,082 G→T 5.4% S435I (AGC→ATC)  metG → methionyl‑tRNA synthetase
RA 2,193,481 C→A 6.6% pseudogene (681/948 nt) yehH → DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
RA 2,202,492 G→T 5.7% T349T (ACG→ACT yehP → VMA domain putative YehL ATPase stimulator
RA 2,210,387 G→T 6.5% P167Q (CCG→CAG)  yehX ← putative ABC superfamily transporter ATP‑binding subunit
RA 2,225,035 G→A 7.6% A180T (GCA→ACA)  yohK → LrgB family inner membrane protein
RA 2,254,067 C→A 5.9% G275C (GGT→TGT)  fruA ← fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
RA 2,261,952 A→G 8.1% K215K (AAA→AAG yeiR → Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
RA 2,268,702 G→T 10.2% D349Y (GAT→TAT)  yejB → microcin C transporter YejABEF, permease subunit; ABC family
RA 2,298,096 T→A 6.0% intergenic (+224/+491) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,097 A→T 7.2% intergenic (+225/+490) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,098 C→T 7.2% intergenic (+226/+489) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,100 C→A 7.2% intergenic (+228/+487) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,101 C→A 7.2% intergenic (+229/+486) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,102 G→A 7.4% intergenic (+230/+485) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,103 C→T 7.2% intergenic (+231/+484) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,104 T→A 7.2% intergenic (+232/+483) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,107 Δ1 bp 7.5% intergenic (+235/+480) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,277 T→C 8.0% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,309,135 C→A 8.0% P723P (CCC→CCA rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,315,187 T→C 5.2% F556L (TTT→CTT)  atoS → sensory histidine kinase in two‑component regulatory system with AtoC
RA 2,316,995 G→T 6.2% G24C (GGC→TGC)  atoD → acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
RA 2,317,013 G→T 5.6% G30C (GGC→TGC)  atoD → acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
RA 2,342,764 C→A 9.8% R63L (CGA→CTA)  inaA ← acid‑inducible Kdo/WaaP family putative kinase
RA 2,368,466 C→A 6.0% W6L (TGG→TTG)  menE ← O‑succinylbenzoate‑CoA ligase
RA 2,398,883 C→A 12.1% intergenic (‑332/+299) nuoA ← / ← lrhA NADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,407,487 G→A 5.6% G180D (GGC→GAC)  ackA → acetate kinase A and propionate kinase 2
RA 2,415,664 C→A 7.2% L160M (CTG→ATG)  yfcH → putative NAD‑dependent nucleotide‑sugar epimerase
RA 2,417,718 G→C 6.9% R91G (CGC→GGC)  hisP ← histidine/lysine/arginine/ornithine transporter subunit
RA 2,474,001 G→T 5.3% G441C (GGC→TGC)  dsdA → D‑serine dehydratase
RA 2,480,217 C→A 8.6% P789T (CCT→ACT)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 2,499,704 G→T 7.0% I136I (ATC→ATA ypdF ← Xaa‑Pro aminopeptidase
RA 2,501,750 C→T 10.6% intergenic (‑29/+190) fryB ← / ← glk putative enzyme IIB component of PTS/glucokinase
RA 2,506,782 G→A 5.6% A88T (GCA→ACA)  nupC → nucleoside (except guanosine) transporter
RA 2,511,452 G→T 13.6% intergenic (‑141/+68) yfeA ← / ← alaX putative diguanylate cyclase/tRNA‑Ala
RA 2,514,609 Δ1 bp 8.5% intergenic (+2/‑3) valY → / → lysV tRNA‑Val/tRNA‑Lys
RA 2,514,875 G→A 6.8% intergenic (+188/+77) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,557,059 G→A 10.7% V37I (GTT→ATT)  yffQ → CPZ‑55 prophage; uncharacterized protein
RA 2,567,817 C→A 8.3% L182L (CTG→CTT eutQ ← RmlC‑like cupin domain protein
RA 2,569,571 C→A 5.5% P722P (CCG→CCT maeB ← malic enzyme: putative oxidoreductase/putative phosphotransacetylase
RA 2,616,125 C→A 7.3% P178Q (CCA→CAA)  purN → phosphoribosylglycinamide formyltransferase 1
RA 2,640,040 G→A 5.6% A215A (GCC→GCT pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain
RA 2,643,327 T→C 5.2% K774E (AAG→GAG)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,643,605 G→T 9.4% S681* (TCA→TAA)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,656,451 C→A 5.5% G78C (GGC→TGC)  trmJ ← tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,667,952 G→T 6.4% L32L (CTC→CTA yphB ← mutarotase superfamily protein, YphB family
RA 2,675,599 G→A 7.8% A169V (GCG→GTG)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,677,370 G→T 7.7% L383L (CTG→CTT yphH → putative DNA‑binding transcriptional regulator
RA 2,716,875 G→A 6.1% E264K (GAG→AAG)  pssA → phosphatidylserine synthase (CDP‑diacylglycerol‑serine O‑phosphatidyltransferase)
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,732,191 G→T 6.1% G363V (GGG→GTG)  pheA → chorismate mutase and prephenate dehydratase, P‑protein
RA 2,733,414 C→A 5.2% L5F (TTG→TTT tyrA ← fused chorismate mutase T/prephenate dehydrogenase
RA 2,747,343 C→A 5.6% intergenic (+38/+24) bamE → / ← ratB lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein
RA 2,761,465 G→T 6.2% E133* (GAG→TAG)  yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,762,144 C→A 8.7% R41S (CGC→AGC)  yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,763,926 T→C 6.6% G41G (GGT→GGC ypjK → CP4‑57 prophage; putative inner membrane protein
RA 2,774,305 A→C 5.3% V594G (GTT→GGT)  ypjA ← adhesin‑like autotransporter
RA 2,776,675 T→G 8.1% intergenic (‑12/+322) pinH ← / ← ypjC pseudogene, invertase resolvase family/pseudogene
RA 2,789,807 C→T 5.9% Q113Q (CAG→CAA ygaU ← uncharacterized protein
RA 2,801,195 G→T 5.1% P235P (CCG→CCT proX → glycine betaine transporter subunit
RA 2,820,575 G→T 6.5% P275P (CCG→CCT srlE → glucitol/sorbitol‑specific enzyme IIB component of PTS
RA 2,856,353 G→T 7.3% T220T (ACG→ACT ygbK → FliA‑regulated DUF1537 family protein
RA 2,896,453 G→T 6.7% R400L (CGA→CTA)  ygcE → putative kinase
RA 2,909,684 C→A 8.9% F423L (TTC→TTA barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 2,911,080 G→T 7.0% G889C (GGT→TGT)  barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 2,914,473 C→T 5.6% P329P (CCG→CCA gudP ← putative D‑glucarate transporter
RA 2,921,997 C→A 5.1% P137Q (CCG→CAG)  sdaC → putative serine transporter
RA 2,927,367 G→T 9.0% intergenic (‑320/‑227) fucA ← / → fucP L‑fuculose‑1‑phosphate aldolase/L‑fucose transporter
RA 2,963,823 C→A 8.7% L15L (CTC→CTA ygdQ → UPF0053 family inner membrane protein
RA 2,970,872 G→A 6.4% A305A (GCG→GCA galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
RA 2,973,746 G→T 5.8% T83T (ACC→ACA ygeA ← Asp/Glu_racemase family protein
RA 2,983,492 G→T 8.6% V76V (GTC→GTA yqeK ← uncharacterized protein
RA 3,006,578 C→A 6.2% E341* (GAA→TAA)  yqeB ← XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
RA 3,007,631 G→T 6.3% intergenic (‑33/+15) yqeB ← / ← yqeC XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold/putative selenium‑dependent hydroxylase accessory protein
RA 3,008,082 C→A 10.7% R112L (CGA→CTA)  yqeC ← putative selenium‑dependent hydroxylase accessory protein
RA 3,032,838 C→A 8.9% E89* (GAA→TAA)  xerD ← site‑specific tyrosine recombinase
RA 3,047,431 G→T 5.6% L257M (CTG→ATG)  pepP ← proline aminopeptidase P II
RA 3,064,718 T→G 5.0% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,088,605 G→T 5.7% G50V (GGG→GTG)  yggS → UPF0001 family protein, PLP‑binding
RA 3,089,811 G→T 6.4% D24Y (GAT→TAT)  yggU → UPF0235 family protein
RA 3,103,043 G→T 7.5% G44G (GGG→GGT yqgA → DUF554 family putative inner membrane protein
RA 3,106,779 C→A 7.0% G20* (GGA→TGA)  yghG ← secretin (GspDbeta) OM localization lipoprotein pilotin
RA 3,107,853 C→A 7.1% intergenic (‑142/+56) pppA ← / ← yghJ bifunctional prepilin leader peptidase/ methylase/DUF4092 family putative lipoprotein peptidase
RA 3,118,182 C→A 5.4% D213Y (GAC→TAC)  glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
RA 3,118,957 G→T 7.1% R309S (CGC→AGC)  glcE ← glycolate oxidase FAD binding subunit
RA 3,152,019 C→A 10.0% L12M (CTG→ATG)  yqhH → outer membrane lipoprotein, Lpp paralog
RA 3,152,221 G→T 7.4% C79F (TGC→TTC)  yqhH → outer membrane lipoprotein, Lpp paralog
RA 3,153,351 G→T 5.5% G385G (GGC→GGA ygiQ ← Radical SAM superfamily protein
RA 3,164,392 C→A 6.0% R184S (CGT→AGT)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB
RA 3,170,082 G→A 6.3% N37N (AAC→AAT cpdA ← 3',5' cAMP phosphodiesterase
RA 3,246,779 C→A 8.3% S265S (TCG→TCT yhaJ ← LysR family putative transcriptional regulator
RA 3,247,161 C→A 5.2% W138L (TGG→TTG)  yhaJ ← LysR family putative transcriptional regulator
RA 3,251,139 C→A 8.9% P77P (CCG→CCT yhaO ← putative transporter
RA 3,254,650 C→A 6.5% L376L (CTG→CTT tdcE ← pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
RA 3,263,315 T→C 6.8% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,264,939 C→A 8.9% W64L (TGG→TTG)  garK ← glycerate kinase I
RA 3,266,827 C→A 8.3% P30P (CCG→CCT garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
RA 3,275,271 G→T 11.6% intergenic (+287/‑64) agaA → / → agaS pseudogene, N‑acetylgalactosamine‑6‑phosphate deacetylase fragment; putative N‑acetylgalactosamine‑6‑phosphate deacetylase/tagatose‑6‑phosphate ketose/aldose isomerase
RA 3,292,078 C→A 6.6% G43V (GGG→GTG)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase
RA 3,294,984 G→T 6.4% L47F (TTG→TTT yhbU → U32 peptidase family protein
RA 3,299,374 G→T 7.8% R616S (CGT→AGT)  deaD ← ATP‑dependent RNA helicase
RA 3,326,754 G→T 5.8% T19T (ACC→ACA rplU ← 50S ribosomal subunit protein L21
RA 3,329,949 G→T 9.8% P72T (CCT→ACT)  ibaG ← acid stress protein; putative transcriptional regulator, BolA family
RA 3,330,996 C→A 5.8% A85A (GCG→GCT mlaC ← ABC transporter maintaining OM lipid asymmetry, periplasmic binding protein
RA 3,350,423 G→T 11.1% Q780H (CAG→CAT gltB → glutamate synthase, large subunit
RA 3,366,506 C→A 7.4% G144W (GGG→TGG)  nanA ← N‑acetylneuraminate lyase
RA 3,375,515 C→A 11.0% intergenic (+46/‑44) degQ → / → degS serine endoprotease, periplasmic/serine endoprotease, periplasmic
RA 3,377,325 C→A 6.8% L101L (CTG→CTT mdh ← malate dehydrogenase, NAD(P)‑binding
RA 3,382,563 C→A 5.8% W45L (TGG→TTG)  aaeX ← DUF1656 family putative inner membrane efflux pump associated protein
RA 3,398,496 C→A 5.9% intergenic (+106/‑299) yrdE → / → accB pseudogene, yedZ paralog/acetyl CoA carboxylase, BCCP subunit
RA 3,404,812 C→A 5.7% P61P (CCC→CCA fis → global DNA‑binding transcriptional dual regulator
RA 3,409,598 G→T 5.6% G403W (GGG→TGG)  acrF → multidrug efflux system protein
RA 3,416,899 A→G 5.9% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,435,656 C→A 10.9% E59* (GAA→TAA)  rpsM ← 30S ribosomal subunit protein S13
RA 3,440,257 C→A 5.5% intergenic (‑14/+1) rpsN ← / ← rplE 30S ribosomal subunit protein S14/50S ribosomal subunit protein L5
RA 3,454,822 C→A 5.1% intergenic (+3/‑5) gspG → / → gspH pseudopilin, cryptic, general secretion pathway/putative general secretory pathway component, cryptic
RA 3,461,873 G→T 5.4% P447Q (CCG→CAG)  chiA ← periplasmic endochitinase
RA 3,488,915 G→T 5.2% G516C (GGC→TGC)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,500,654 C→A 6.0% T14T (ACG→ACT yhfT ← inner membrane protein
RA 3,501,567 G→T 5.7% T128N (ACC→AAC)  php ← phosphotriesterase homology protein
RA 3,512,679 C→A 8.9% intergenic (‑256/+145) aroK ← / ← hofQ shikimate kinase I/DNA catabolic putative fimbrial transporter
RA 3,514,870 G→T 6.3% A154A (GCC→GCA hofN ← DNA catabolic putative fimbrial assembly protein
RA 3,532,952 G→T 6.6% E737* (GAA→TAA)  yhgF → putative transcriptional accessory protein
RA 3,547,438 G→T 5.5% C332F (TGC→TTC)  malT → mal regulon transcriptional activator
RA 3,549,659 C→A 6.3% E184* (GAG→TAG)  rtcA ← RNA 3'‑terminal phosphate cyclase
RA 3,564,744 G→T 5.0% R707S (CGT→AGT)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,617,481 G→T 14.3% pseudogene (235/351 nt) yrhC → pseudogene fragment
RA 3,659,586 G→T 7.5% P452Q (CCG→CAG)  gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,661,396 C→A 6.1% G385C (GGT→TGT)  yhjA ← putative cytochrome C peroxidase
RA 3,669,589 A→G 9.1% intergenic (+121/+61) yhjE → / ← yhjG MFS superfamily sugar transport 1 family protein/putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,669,592 C→T 8.0% intergenic (+124/+58) yhjE → / ← yhjG MFS superfamily sugar transport 1 family protein/putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,669,597 C→T 7.5% intergenic (+129/+53) yhjE → / ← yhjG MFS superfamily sugar transport 1 family protein/putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,669,600 A→G 5.1% intergenic (+132/+50) yhjE → / ← yhjG MFS superfamily sugar transport 1 family protein/putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,672,940 G→T 6.3% L54L (CTG→CTT kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,691,055 C→A 5.3% P413H (CCT→CAT)  bcsE → cellulose production protein
RA 3,705,323 G→T 8.8% I15I (ATC→ATA yhjX ← pyruvate‑inducible inner membrane protein, putative transporter
RA 3,742,844 C→A 10.0% R85S (CGT→AGT)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,752,670 G→T 5.7% T184T (ACC→ACA selB ← selenocysteinyl‑tRNA‑specific translation factor
RA 3,785,919 C→A 5.4% intergenic (‑8/+267) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown
RA 3,785,965 C→A 7.7% intergenic (‑54/+221) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown
RA 3,803,975 C→A 8.4% G180W (GGG→TGG)  mutM ← formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
RA 3,818,141 G→T 6.1% Q89H (CAG→CAT trmH → tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent
RA 3,821,819 C→T 8.4% P69P (CCG→CCA gltS ← glutamate transporter
RA 3,844,126 T→A 6.5% intergenic (‑40/+36) uhpA ← / ← ilvN response regulator in two‑component regulatory system wtih UhpB/acetolactate synthase 1 small subunit
RA 3,845,393 G→T 5.6% P251Q (CCA→CAA)  ilvB ← acetolactate synthase 2 large subunit
RA 3,857,229 C→A 5.3% intergenic (‑266/‑30) glvC ← / → yidP pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component/UTRA domain‑containing GntR family putative transcriptional regulator
RA 3,877,923 A→G 100% Q24R (CAG→CGG)  rnpA → protein C5 component of RNase P
RA 3,880,560 C→A 8.3% Q125K (CAG→AAG)  mnmE → tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
RA 3,887,412 G→T 6.8% E58* (GAA→TAA)  yieE → phosphopantetheinyl transferase superfamily protein
RA 3,918,498 C→A 5.2% G166C (GGT→TGT)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,923,726 G→T 6.4% P243Q (CCG→CAG)  ravA ← hexameric AAA+ MoxR family ATPase, putative molecular chaperone
RA 3,979,767 C→A 7.3% intergenic (‑400/‑25) aslA ← / → glmZ putative Ser‑type periplasmic non‑aryl sulfatase/sRNA antisense activator of glmS mRNA, Hfq‑dependent
RA 3,988,103 C→A 8.1% intergenic (+18/‑19) yifL → / → dapF putative lipoprotein/diaminopimelate epimerase
RA 4,000,025 G→A 6.9% A268T (GCC→ACC)  recQ → ATP‑dependent DNA helicase
RA 4,008,201 C→A 8.5% Q597K (CAG→AAG)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,019,993 Δ1 bp 100% coding (107/702 nt) fre → NAD(P)H‑flavin reductase
RA 4,049,183 C→A 10.2% E173* (GAA→TAA)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,063,086 G→T 9.7% H226N (CAC→AAC)  yihR ← mutarotase superfamily protein, YihR family
RA 4,064,210 C→A 6.6% D303Y (GAC→TAC)  yihS ← aldose‑ketose isomerase; D‑mannose isomerase
RA 4,067,579 G→T 7.8% G161W (GGG→TGG)  yihV → putative sugar kinase
RA 4,072,790 C→A 8.8% P45Q (CCG→CAG)  yiiE → CopG family putative transcriptional regulator
RA 4,075,612 C→A 5.2% G170W (GGG→TGG)  fdoH ← formate dehydrogenase‑O, Fe‑S subunit
RA 4,094,391 G→T 11.5% T171T (ACC→ACA cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,107,842 G→T 8.6% T59T (ACC→ACA glpF ← glycerol facilitator
RA 4,129,376 C→A 5.2% P87P (CCG→CCT fsaB ← fructose‑6‑phosphate aldolase 2
RA 4,148,050 C→A 6.5% L425M (CTG→ATG)  argH → argininosuccinate lyase
RA 4,165,450 A→C 5.5% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,172,432 G→T 6.5% L420L (CTG→CTT rpoB → RNA polymerase, beta subunit
RA 4,172,857 A→T 60.0% E562V (GAA→GTA)  rpoB → RNA polymerase, beta subunit
RA 4,173,032 C→A 6.2% N620K (AAC→AAA rpoB → RNA polymerase, beta subunit
RA 4,176,469 A→G 10.2% K398E (AAG→GAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,177,586 T→G 8.3% L770R (CTC→CGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 7.5% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,286,083 T→C 20.9% intergenic (+361/+170) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,305,314 G→T 5.8% T14T (ACC→ACA phnO ← aminoalkylphosphonate N‑acetyltransferase
RA 4,305,615 G→T 9.3% V95V (GTC→GTA phnN ← ribose 1,5‑bisphosphokinase
RA 4,306,244 G→T 6.0% G264G (GGC→GGA phnM ← ribophosphonate triphosphate hydrolase
RA 4,310,740 G→T 6.2% L109I (CTT→ATT)  phnH ← ribophosphonate triphosphate synthase subunit
RA 4,312,829 C→T 5.7% pseudogene (219/426 nt) phnE ← defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
RA 4,353,379 G→T 13.3% P494Q (CCG→CAG)  dsbD ← fused thiol:disulfide interchange protein: activator of DsbC/conserved protein
RA 4,360,595 G→A 5.9% A31T (GCA→ACA)  groS → Cpn10 chaperonin GroES, small subunit of GroESL
RA 4,369,709 C→T 5.4% G209D (GGC→GAC)  frdB ← fumarate reductase (anaerobic), Fe‑S subunit
RA 4,379,534 C→T 6.5% G215D (GGC→GAC)  psd ← phosphatidylserine decarboxylase
RA 4,394,008 C→A 6.3% R298S (CGT→AGT)  hflC → modulator for HflB protease specific for phage lambda cII repressor
RA 4,401,481 G→A 6.2% A121A (GCG→GCA yjfK → DUF2491 family protein
RA 4,412,376 G→T 9.5% W125L (TGG→TTG)  ulaD → 3‑keto‑L‑gulonate 6‑phosphate decarboxylase
RA 4,417,781 C→A 6.1% pseudogene (271/402 nt) ytfA → pseudogene, related to transcriptional regulators
RA 4,425,130 G→T 5.2% P418Q (CCG→CAG)  cpdB ← 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase
RA 4,440,247 G→T 7.2% G157C (GGC→TGC)  ytfQ → galactofuranose binding proteint: periplasmic‑binding component of ABC superfamily
RA 4,447,390 C→A 8.4% R98L (CGT→CTT)  yjgA ← ribosome‑associated UPF0307 family protein
RA 4,501,050 G→T 6.8% A75A (GCC→GCA fecE ← iron‑dicitrate transporter subunit
RA 4,501,512 C→A 5.5% V240V (GTG→GTT fecD ← iron‑dicitrate transporter subunit
RA 4,501,527 C→A 5.6% M235I (ATG→ATT fecD ← iron‑dicitrate transporter subunit
RA 4,505,463 G→T 5.4% P344P (CCC→CCA fecA ← ferric citrate outer membrane transporter
RA 4,507,300 G→T 7.1% R79S (CGC→AGC)  fecR ← FecI pro‑sigma factor; transmembrane signal transducer for ferric citrate transport; periplasmic FecA:ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
RA 4,508,633 A→G 7.2% pseudogene (96/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,519,580 C→T 5.1% A161T (GCA→ACA)  sgcC ← putative PTS system EIIC permease component
RA 4,522,568 G→T 7.5% Q145K (CAG→AAG)  yjhP ← putative methyltransferase
RA 4,557,503 C→A 6.8% P278P (CCG→CCT mdtM ← multidrug efflux system protein
RA 4,565,199 C→A 7.8% pseudogene (1464/2937 nt) yjiV → pseudogene; conserved hypothetical protein
RA 4,588,521 C→A 7.6% L246L (CTG→CTT opgB ← phosphoglycerol transferases I and II
RA 4,604,195 A→G 5.7% intergenic (+118/+302) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,609,231 C→A 5.6% Q396K (CAG→AAG)  deoA → thymidine phosphorylase
RA 4,610,011 C→A 5.9% R198S (CGT→AGT)  deoB → phosphopentomutase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 463832 464289 458 17 [12] [15] 18 [ybaO] [ybaO]
* * ÷ CP009273 578893 579140 248 18 [16] [12] 18 [appY] [appY]
* * ÷ CP009273 2027284 2027428 145 17 [16] [15] 17 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein
* * ÷ CP009273 3448752 3448865 114 23 [15] [13] 29 [gspA] [gspA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 335746 =124 (1.580)11 (0.140) 6/248 NT 8.2% noncoding (279/358 nt) REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences
?CP009273 372778 = NA (NA)intergenic (+11/+213) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 360104 =55 (0.700)85 (1.090) 32/250 NT 60.7% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370386 =NA (NA)15 (0.290) 6/164 NT 14.5% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372978 = 98 (1.720)intergenic (+211/+13) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 370479 =68 (0.870)6 (0.120) 3/166 NT 8.1% noncoding (94/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372986 = 92 (1.780)intergenic (+219/+5) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 1203246 =33 (0.420)28 (0.410) 15/218 NT 53.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 21 (0.310)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326129 (0.370)10 (0.150) 7/218 NT 30.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 21 (0.310)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1972769 =1 (0.010)55 (0.710) 30/248 NT 98.2% intergenic (‑315/‑465) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 19727774 (0.050)83 (1.060) 35/250 NT 95.4% intergenic (‑323/‑457) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E