breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 248 C→T 9.6% A20V (GCG→GTG)  thrL → thr operon leader peptide
RA 12,110 C→A 5.6% intergenic (‑324/‑53) yaaI ← / → dnaK UPF0412 family protein/chaperone Hsp70, with co‑chaperone DnaJ
RA 75,348 G→A 5.1% A198V (GCG→GTG)  leuD ← 3‑isopropylmalate dehydratase small subunit
RA 94,323 G→T 5.9% R250R (CGG→CGT murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
RA 96,239 C→A 5.4% R37S (CGC→AGC)  murG → N‑acetylglucosaminyl transferase
RA 104,013 C→A 14.6% intergenic (+52/‑179) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
RA 114,800 G→T 6.8% S111S (TCC→TCA nadC ← quinolinate phosphoribosyltransferase
RA 114,842 G→T 6.9% V97V (GTC→GTA nadC ← quinolinate phosphoribosyltransferase
RA 121,390 C→A 12.0% S629S (TCC→TCA aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
RA 142,444 C→A 8.6% R293S (CGC→AGC)  yadE → putative polysaccharide deacetylase lipoprotein
RA 153,209 G→T 6.6% I54I (ATC→ATA yadN ← putative fimbrial‑like adhesin protein
RA 165,108 G→A 6.9% V380I (GTT→ATT)  fhuA → ferrichrome outer membrane transporter
RA 177,670 C→T 6.8% G100G (GGC→GGT degP → serine endoprotease (protease Do), membrane‑associated
RA 185,449 T→C 6.1% Q182R (CAG→CGG)  map ← methionine aminopeptidase
RA 189,367 C→T 6.6% S3S (AGC→AGT frr → ribosome recycling factor
RA 200,351 C→A 11.4% P173T (CCA→ACA)  lpxB → tetraacyldisaccharide‑1‑P synthase
RA 221,402 A→C 5.7% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 235,769 G→T 8.3% pseudogene (177/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 238,320 Δ1 bp 8.9% coding (1471/2445 nt) fadE ← acyl coenzyme A dehydrogenase
RA 238,321 Δ1 bp 8.7% coding (1470/2445 nt) fadE ← acyl coenzyme A dehydrogenase
RA 238,324 G→A 8.7% G489G (GGC→GGT fadE ← acyl coenzyme A dehydrogenase
RA 238,327 C→A 8.7% Q488H (CAG→CAT) ‡ fadE ← acyl coenzyme A dehydrogenase
RA 238,328 T→C 8.8% Q488R (CAG→CGG) ‡ fadE ← acyl coenzyme A dehydrogenase
RA 238,330 G→A 8.7% Y487Y (TAC→TAT fadE ← acyl coenzyme A dehydrogenase
RA 238,333 A→C 9.8% A486A (GCT→GCG) ‡ fadE ← acyl coenzyme A dehydrogenase
RA 238,334 G→C 9.8% A486G (GCT→GGT) ‡ fadE ← acyl coenzyme A dehydrogenase
RA 238,337 Δ1 bp 9.8% coding (1454/2445 nt) fadE ← acyl coenzyme A dehydrogenase
RA 242,325 G→A 7.5% intergenic (‑33/+123) yafK ← / ← yafQ L,D‑transpeptidase‑related protein/translation inhibitor toxin of toxin‑antitoxin pair YafQ/DinJ
RA 243,622 G→T 11.7% D142Y (GAT→TAT)  yafL → putative lipoprotein and C40 family peptidase
RA 256,248 G→T 10.7% S50S (TCG→TCT proB → gamma‑glutamate kinase
RA 262,526 C→T 8.9% pseudogene (153/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 262,529 C→T 8.8% pseudogene (150/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 262,691 G→A 7.4% intergenic (‑13/+204) ykfK ← / ← yafZ CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related/CP4‑6 prophage; conserved protein
RA 262,692 T→G 7.3% intergenic (‑14/+203) ykfK ← / ← yafZ CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related/CP4‑6 prophage; conserved protein
RA 263,285 G→A 14.8% D144D (GAC→GAT yafZ ← CP4‑6 prophage; conserved protein
RA 263,324 A→G 8.4% G131G (GGT→GGC yafZ ← CP4‑6 prophage; conserved protein
RA 292,279 C→A 7.7% G177* (GGA→TGA)  intF ← CP4‑6 prophage; putative phage integrase
RA 296,628 G→A 5.8% P6P (CCC→CCT paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 315,164 C→A 9.5% K183N (AAG→AAT rclA ← reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 322,367 C→T 5.8% A198T (GCC→ACC)  betA ← choline dehydrogenase, a flavoprotein
RA 331,147 G→T 10.9% A53S (GCT→TCT)  yahD → ankyrin repeat protein
RA 335,689 A→G 7.7% intergenic (+235/‑187) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 335,690 G→A 9.3% intergenic (+236/‑186) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 335,696 C→T 7.4% intergenic (+242/‑180) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 335,703 C→T 9.0% intergenic (+249/‑173) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 335,710 C→T 7.0% intergenic (+256/‑166) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 340,599 G→T 9.3% R238I (AGA→ATA)  yahL → uncharacterized protein
RA 345,326 T→C 5.3% intergenic (+43/‑397) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 346,154 C→A 6.1% S144R (AGC→AGA prpC → 2‑methylcitrate synthase
RA 364,470 C→A 6.1% R543S (CGC→AGC)  mhpA → 3‑(3‑hydroxyphenyl)propionate hydroxylase
RA 370,415 C→T 5.4% intergenic (+78/‑500) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 372,599 C→A 7.1% V124V (GTC→GTA yaiL → DUF2058 family protein
RA 372,807 Δ1 bp 6.4% intergenic (+40/+184) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,808 G→A 6.6% intergenic (+41/+183) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,906 T→A 5.3% intergenic (+139/+85) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,930 C→T 8.1% intergenic (+163/+61) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,934 C→T 9.0% intergenic (+167/+57) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,948 G→A 7.4% intergenic (+181/+43) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 379,901 C→A 8.8% G58C (GGC→TGC)  yaiS ← putative PIG‑L family deacetylase
RA 408,943 G→A 6.0% T756I (ACC→ATC)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 449,252 C→A 10.1% A124A (GCG→GCT yajG ← putative lipoprotein
RA 465,800 G→A 6.1% E492K (GAA→AAA)  mdlA → putative multidrug transporter subunit of ABC superfamily, ATP‑binding component
RA 484,485 G→T 5.5% E832* (GAA→TAA)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated
RA 497,644 G→T 5.1% L351M (CTG→ATG)  ybaL ← inner membrane putative NAD(P)‑binding transporter
RA 507,612 C→A 6.7% A172A (GCC→GCA glsA → glutaminase 1
RA 507,734 C→A 5.2% A213E (GCG→GAG)  glsA → glutaminase 1
RA 527,360 C→T 9.3% Q106Q (CAG→CAA allS ← allD operon transcriptional activator
RA 546,321 C→A 8.0% Q143K (CAG→AAG)  ybcF → putative carbonate kinase
RA 628,446 G→T 19.0% P163Q (CCG→CAG)  ybdH ← putative oxidoreductase
RA 629,236 C→A 8.7% P65P (CCC→CCA ybdL → methionine aminotransferase, PLP‑dependent
RA 631,455 G→T 10.5% P191T (CCC→ACC)  ybdN ← PAPS reductase‑like domain protein
RA 639,144 C→A 6.6% V94L (GTA→TTA)  rnk ← regulator of nucleoside diphosphate kinase
RA 647,263 T→C 5.7% K17R (AAA→AGA)  citC ← [citrate [pro‑3S]‑lyase] ligase
RA 699,972 G→T 6.6% Q191H (CAG→CAT nagE → fused N‑acetyl glucosamine specific PTS enzyme: IIC, IIB, and IIA components
RA 710,750 A→C 14.9% intergenic (+96/‑118) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,751 G→A 14.8% intergenic (+97/‑117) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,753 A→C 15.4% intergenic (+99/‑115) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,755 G→C 15.4% intergenic (+101/‑113) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,756 G→C 15.4% intergenic (+102/‑112) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,774 A→T 12.9% intergenic (+120/‑94) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,775 A→C 12.9% intergenic (+121/‑93) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,776 G→A 11.1% intergenic (+122/‑92) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,777 C→T 10.5% intergenic (+123/‑91) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 710,779 A→C 11.0% intergenic (+125/‑89) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 714,905 G→T 8.3% L338M (CTG→ATG)  speF ← ornithine decarboxylase isozyme, inducible
RA 723,488 C→T 7.7% G234D (GGC→GAC)  kdpA ← potassium translocating ATPase, subunit A
RA 725,257 T→C 8.4% I73I (ATT→ATC rhsC → Rhs family putative polymorphic toxin
RA 726,319 G→C 5.7% A427A (GCG→GCC rhsC → Rhs family putative polymorphic toxin
RA 726,848 A→G 5.1% T604A (ACC→GCC)  rhsC → Rhs family putative polymorphic toxin
RA 727,207 C→T 6.2% R723R (CGC→CGT rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 8.1% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 728,446 A→G 7.5% V1136V (GTA→GTG rhsC → Rhs family putative polymorphic toxin
RA 728,467 T→C 8.2% I1143I (ATT→ATC rhsC → Rhs family putative polymorphic toxin
RA 732,050 C→T 10.0% pseudogene (150/240 nt) ybfQ → pseudogene, H repeat‑associated protein
RA 732,499 T→C 9.1% pseudogene (219/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 734,222 T→C 6.4% I225I (ATT→ATC ybfD → H repeat‑associated putative transposase
RA 749,509 G→A 7.4% T139I (ACC→ATC)  gltA ← citrate synthase
RA 776,710 A→T 8.5% intergenic (+32/‑115) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 785,106 G→T 14.0% F110L (TTC→TTA galK ← galactokinase
RA 813,288 G→A 5.3% L263L (CTG→CTA moaA → molybdopterin biosynthesis protein A
RA 814,665 C→A 7.8% A54A (GCC→GCA moaD → molybdopterin synthase, small subunit
RA 835,682 C→T 5.7% G436S (GGC→AGC)  fiu ← catecholate siderophore receptor Fiu
RA 847,291 C→A 7.5% D255Y (GAC→TAC)  ybiP ← putative EptAB family phosphoethanolamine transferase, inner membrane protein
RA 851,355:1 +G 5.5% intergenic (‑155/‑64) ldtB ← / → ybiT L,D‑transpeptidase linking Lpp to murein/putative transporter subunit of ABC superfamily: ATP‑binding component
RA 851,355:2 +C 5.5% intergenic (‑155/‑64) ldtB ← / → ybiT L,D‑transpeptidase linking Lpp to murein/putative transporter subunit of ABC superfamily: ATP‑binding component
RA 851,355:3 +T 5.5% intergenic (‑155/‑64) ldtB ← / → ybiT L,D‑transpeptidase linking Lpp to murein/putative transporter subunit of ABC superfamily: ATP‑binding component
RA 860,939 G→T 6.4% F294L (TTC→TTA moeA ← molybdopterin molybdenumtransferase; molybdopterin biosynthesis protein
RA 916,429 G→T 5.3% Q209H (CAG→CAT macB → fused macrolide transporter subunits of ABC superfamily: ATP‑binding component/membrane component
RA 944,862 G→T 7.4% R55R (CGA→AGA)  ycaN ← LysR family putative transcriptional regulator
RA 956,649 A→G 9.2% intergenic (+165/‑8) ycaL → / → cmk putative peptidase‑related chaperone/cytidylate kinase
RA 960,135 G→T 7.7% P120P (CCG→CCT ycaI → ComEC family inner membrane protein
RA 972,699 G→A 8.9% Q306Q (CAG→CAA mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 973,565 A→G 6.7% Q595R (CAG→CGG)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 979,098 G→T 7.8% intergenic (+19/‑8) ycbK → / → ycbL M15A protease‑related family periplasmic protein/putative metal‑binding enzyme
RA 980,635 G→T 8.2% F177L (TTC→TTA aspC ← aspartate aminotransferase, PLP‑dependent
RA 981,730 C→T 5.7% A237T (GCT→ACT)  ompF ← outer membrane porin 1a (Ia;b;F)
RA 990,013 C→A 9.0% W92C (TGG→TGT ssuC ← putative alkanesulfonate transporter subunit
RA 997,443 G→T 7.5% G61W (GGG→TGG)  elfG → putative fimbrial‑like adhesin protein
RA 1,005,202 C→A 7.0% H635N (CAT→AAT)  rlmL → fused 23S rRNA m(2)G2445 and m(7)G2069 methyltransferase, SAM‑dependent
RA 1,012,498 G→T 8.8% P420T (CCC→ACC)  ycbZ ← putative peptidase
RA 1,063,559 C→A 6.6% S8Y (TCC→TAC)  ymdF → KGG family protein
RA 1,064,481 C→A 8.2% G272V (GGG→GTG)  rutG ← pyrimidine permease
RA 1,127,021 G→T 9.1% R43L (CGG→CTG)  flgC → flagellar component of cell‑proximal portion of basal‑body rod
RA 1,127,693 G→A 9.5% L128L (CTG→CTA flgD → flagellar hook assembly protein
RA 1,129,692 G→A 6.2% A145A (GCG→GCA flgF → flagellar component of cell‑proximal portion of basal‑body rod
RA 1,139,290 C→A 14.8% E178D (GAG→GAT rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
RA 1,141,503 C→T 6.0% M183I (ATG→ATA yceF ← Maf‑like protein
RA 1,191,748 C→T 7.7% T390T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,218,936 C→T 5.3% intergenic (+31/‑84) ymgJ → / → ycgI uncharacterized protein/pseudogene
RA 1,219,041 G→A 5.1% pseudogene (22/108 nt) ycgI → pseudogene
RA 1,219,085 T→C 5.3% pseudogene (66/108 nt) ycgI → pseudogene
RA 1,264,818 G→T 9.4% noncoding (40/67 nt) rdlA → sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA
RA 1,265,888 T→G 5.3% noncoding (40/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,266,001 G→T 9.4% intergenic (+85/+204) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
RA 1,277,758 G→T 11.8% A813A (GCG→GCT narG → nitrate reductase 1, alpha subunit
RA 1,282,249 A→G 17.9% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,283,409 C→A 8.2% L224L (CTG→CTT purU ← formyltetrahydrofolate hydrolase
RA 1,296,971 C→A 9.7% Y511* (TAC→TAA oppA → oligopeptide transporter subunit
RA 1,297,112 G→T 6.3% intergenic (+42/‑44) oppA → / → oppB oligopeptide transporter subunit/oligopeptide transporter subunit
RA 1,298,034 C→A 6.5% V293V (GTC→GTA oppB → oligopeptide transporter subunit
RA 1,298,059 C→A 6.4% P302T (CCG→ACG)  oppB → oligopeptide transporter subunit
RA 1,341,727 G→T 10.5% P481P (CCC→CCA rnb ← ribonuclease II
RA 1,347,987 C→T 6.4% P263P (CCG→CCA sapC ← antimicrobial peptide transport ABC transporter permease
RA 1,363,269 G→T 8.6% W71L (TGG→TTG)  pspB → psp operon transcription co‑activator
RA 1,363,596 C→A 5.2% V105V (GTC→GTA pspC → psp operon transcription co‑activator
RA 1,367,098 G→T 7.7% E311D (GAG→GAT ycjN → putative ABC superfamily sugar transporter periplasmic‑binding protein
RA 1,384,753 G→T 10.3% P35T (CCT→ACT)  mpaA ← murein peptide amidase A
RA 1,422,040 A→G 5.8% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,098 G→A 8.2% pseudogene (886/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,132 C→T 5.9% pseudogene (852/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,150 C→T 5.4% pseudogene (834/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,168 A→C 5.2% pseudogene (816/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 7.3% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 9.1% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 7.0% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,432,890 G→T 7.7% P718T (CCT→ACT)  pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,460,467 G→A 7.1% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,483,438 C→A 18.3% P317H (CCC→CAC)  aldA → aldehyde dehydrogenase A, NAD‑linked
RA 1,516,426 C→A 5.6% M299I (ATG→ATT yncD ← putative iron outer membrane transporter
RA 1,527,113 T→G 9.7% D347E (GAT→GAG ydcC → H repeat‑associated putative transposase
RA 1,536,006 A→G 8.3% I281T (ATT→ACT)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,541,970 G→T 9.7% E105* (GAA→TAA)  fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,544,819 C→A 5.8% I34I (ATC→ATA fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,588,049 C→A 5.8% pseudogene (4196/5323 nt) yneO ← pseudogene, AidA homolog
RA 1,629,321 G→T 7.7% H262N (CAC→AAC)  stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,664,783 G→T 5.2% V276V (GTG→GTT ynfM → putative arabinose efflux transporter
RA 1,668,943 C→A 5.6% F258L (TTC→TTA tqsA → pheromone AI‑2 transporter
RA 1,679,430 G→A 7.4% L305L (CTG→CTA tus → inhibitor of replication at Ter, DNA‑binding protein
RA 1,685,588 C→A 8.3% P494T (CCG→ACG)  ydgA → DUF945 family protein
RA 1,691,520 C→A 8.6% intergenic (‑211/+10) uidR ← / ← hdhA transcriptional repressor/7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent
RA 1,700,347 G→T 6.9% P108P (CCG→CCT rsxA → SoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex
RA 1,720,698 C→A 6.2% A140E (GCG→GAG)  nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,720,789 G→T 13.0% A170A (GCG→GCT nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
RA 1,743,166 C→A 8.3% T217T (ACG→ACT ydhT ← FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
RA 1,757,890 G→A 9.2% A126V (GCG→GTG)  sufB ← component of SufBCD Fe‑S cluster assembly scaffold
RA 1,772,524 G→T 7.8% E358* (GAA→TAA)  ydiO → putative acyl‑CoA dehydrogenase
RA 1,785,446 G→A 6.1% A19V (GCA→GTA)  ydiU ← UPF0061 family protein
RA 1,789,351 G→A 23.1% S20L (TCA→TTA)  btuC ← vitamin B12 transporter subunit: membrane component of ABC superfamily
RA 1,829,328 C→A 6.4% A88A (GCC→GCA ydjZ → TVP38/TMEM64 family inner membrane protein
RA 1,835,917 G→T 7.0% L57L (CTG→CTT nudG → CTP pyrophosphohydrolase; also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
RA 1,843,183 C→A 12.6% F30L (TTC→TTA sppA → protease IV (signal peptide peptidase)
RA 1,849,640 G→T 10.9% R197R (CGG→AGG)  ydjG ← alpha‑Keto reductase, NADH‑dependent; can use methylglyoxal as substrate
RA 1,876,522 G→T 6.4% P118P (CCG→CCT dmlA → D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase
RA 1,879,285 C→A 12.1% L122M (CTG→ATG)  yeaW → putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster
RA 1,894,952 C→T 5.5% S297S (TCG→TCA yoaE ← putative membrane protein/conserved protein
RA 1,897,932 C→A 5.4% I198I (ATC→ATA manY → mannose‑specific enzyme IIC component of PTS
RA 1,922,023 C→A 9.1% P358P (CCG→CCT ptrB ← protease II
RA 1,924,208 A→G 8.7% intergenic (‑244/+83) yebE ← / ← yebF DUF533 family inner membrane protein/extracellular Colicin M immunity family protein
RA 1,928,233 G→T 8.5% S210* (TCA→TAA)  edd ← 6‑phosphogluconate dehydratase
RA 1,930,463 C→A 8.5% G37C (GGT→TGT)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase
RA 1,936,274 C→T 20.7% T189T (ACG→ACA znuA ← zinc transporter subunit: periplasmic‑binding component of ABC superfamily
RA 1,941,388 G→T 5.6% G82G (GGC→GGA ruvC ← component of RuvABC resolvasome, endonuclease
RA 1,992,667 G→A 13.6% L99L (CTG→TTG)  dcyD ← D‑cysteine desulfhydrase, PLP‑dependent
RA 2,033,508 G→T 10.9% A184S (GCA→TCA)  yedY → membrane‑anchored, periplasmic TMAO, DMSO reductase
RA 2,039,935 T→A 5.4% V506D (GTC→GAC)  yeeJ → putative adhesin
RA 2,066,171 C→A 7.5% G384G (GGC→GGA flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,074,417 G→T 7.5% P109P (CCC→CCA sbmC ← DNA gyrase inhibitor
RA 2,077,127 G→T 7.0% P297P (CCG→CCT sbcB → exodeoxyribonuclease I; exonuclease I
RA 2,082,069 C→A 6.1% G181C (GGT→TGT)  yeeZ ← putative epimerase
RA 2,087,757 C→A 6.7% A270E (GCG→GAG)  hisB → fused histidinol‑phosphatase/imidazoleglycerol‑phosphate dehydratase
RA 2,088,363 G→T 6.4% M116I (ATG→ATT hisH → imidazole glycerol phosphate synthase, glutamine amidotransferase subunit with HisF
RA 2,090,712 G→T 13.3% intergenic (+6/+90) hisI → / ← wzzB fused phosphoribosyl‑AMP cyclohydrolase/phosphoribosyl‑ATP pyrophosphatase/regulator of length of O‑antigen component of lipopolysaccharide chains
RA 2,114,428 C→A 8.3% G203V (GGC→GTC)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase
RA 2,134,522 C→A 8.0% R191L (CGG→CTG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,198,216 C→A 7.6% L48I (CTT→ATT)  yehL → putative ABC superfamily transporter ATP‑binding subunit
RA 2,207,425 C→A 14.9% M233I (ATG→ATT yehU ← inner membrane putative sensory kinase in two‑component system with YehT
RA 2,212,592 G→T 13.2% P123P (CCC→CCA osmF ← putative ABC superfamily transporter periplasmic‑binding protein
RA 2,227,142 G→T 6.1% E22* (GAA→TAA)  yeiS → DUF2542 family protein
RA 2,249,666 G→A 8.3% R383R (CGC→CGT psuT ← putative nucleoside transporter
RA 2,249,669 T→G 8.3% K382N (AAA→AAC) ‡ psuT ← putative nucleoside transporter
RA 2,249,671 T→G 8.3% K382Q (AAA→CAA) ‡ psuT ← putative nucleoside transporter
RA 2,325,658 G→T 5.9% pseudogene (2224/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,337,131 C→T 5.6% G173D (GGC→GAC)  yfaL ← adhesin
RA 2,345,469 G→T 9.8% L129I (CTC→ATC)  glpT ← sn‑glycerol‑3‑phosphate transporter
RA 2,347,820 T→C 9.9% L26P (CTG→CCG)  glpB → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit
RA 2,349,059 G→A 5.5% P20P (CCG→CCA glpC → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster
RA 2,349,887 C→A 7.0% H296Q (CAC→CAA glpC → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster
RA 2,379,637 A→G 5.9% Y100C (TAT→TGT)  yfbL → putative peptidase
RA 2,395,893 C→A 8.0% E478* (GAG→TAG)  nuoC ← NADH:ubiquinone oxidoreductase, fused CD subunit
RA 2,436,464 C→A 9.6% P408T (CCA→ACA)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,450,011 G→T 8.7% A94E (GCG→GAG)  sixA ← phosphohistidine phosphatase
RA 2,457,936 T→A 9.7% K184I (AAA→ATA)  mlaA ← ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
RA 2,464,610 A→T 7.5% T129T (ACT→ACA yfdK ← CPS‑53 (KpLE1) prophage; conserved protein
RA 2,465,527 C→T 6.4% pseudogene (75/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,492,873 G→A 13.3% D242N (GAT→AAT)  ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
RA 2,493,691 G→T 7.5% P514P (CCG→CCT ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
RA 2,503,245 C→A 8.5% P46Q (CCG→CAG)  yfeO → putative ion channel protein
RA 2,503,453 C→A 5.4% V115V (GTC→GTA yfeO → putative ion channel protein
RA 2,514,609 Δ1 bp 14.0% intergenic (+2/‑3) valY → / → lysV tRNA‑Val/tRNA‑Lys
RA 2,545,478 C→T 8.0% R7H (CGC→CAC)  ypeA ← putative acyl‑CoA transferase
RA 2,603,210 C→A 6.7% F455L (TTC→TTA hyfG → hydrogenase 4, subunit
RA 2,613,836 C→A 5.6% M132I (ATG→ATT upp ← uracil phosphoribosyltransferase
RA 2,620,217 C→T 5.3% R694Q (CGA→CAA)  yfgF ← cyclic‑di‑GMP phosphodiesterase, anaerobic
RA 2,643,036 G→T 6.6% P871T (CCC→ACC)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
RA 2,666,996 C→A 7.1% H98N (CAT→AAT)  yphA → DoxX family inner membrane protein
RA 2,669,347 C→A 9.8% G280C (GGT→TGT)  yphD ← inner membrane putative ABC superfamily sugar transporter permease
RA 2,682,911 A→T 6.2% intergenic (‑46/+119) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,682,916 C→T 5.5% intergenic (‑51/+114) yfhG ← / ← glrK putative outer membrane protein modulating the QseEF response/sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,688,637 G→T 6.9% S89* (TCG→TAG)  purL ← phosphoribosylformyl‑glycineamide synthetase
RA 2,719,426 T→C 86.8% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,764,424 C→A 7.4% H129N (CAT→AAT)  yfjS → CP4‑57 prophage; uncharacterized protein
RA 2,782,570 C→T 6.6% A76V (GCG→GTG)  csiD → carbon starvation protein
RA 2,811,122 T→C 5.2% intergenic (‑260/+21) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
RA 2,811,129 G→A 5.5% intergenic (‑267/+14) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
RA 2,811,131 Δ1 bp 5.3% intergenic (‑269/+12) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
RA 2,811,136 G→T 5.3% intergenic (‑274/+7) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
RA 2,837,370 C→A 5.6% R67L (CGA→CTA)  hycG ← hydrogenase 3 and formate hydrogenase complex, HycG subunit
RA 2,842,651 C→A 16.5% T186T (ACG→ACT hycB ← hydrogenase 3, Fe‑S subunit
RA 2,851,839 T→C 5.3% L463P (CTG→CCG)  mutS → methyl‑directed mismatch repair protein
RA 2,878,529 T→A 6.0% T684S (ACC→TCC)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
RA 2,887,488 C→A 7.1% Q71K (CAG→AAG)  ygcO → putative 4Fe‑4S cluster‑containing protein
RA 2,913,215 C→A 10.7% M297I (ATG→ATT gudX ← glucarate dehydratase‑related protein, substrate unknown
RA 2,959,246 C→A 11.1% G51C (GGC→TGC)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
RA 2,978,427 C→A 5.3% Q175H (CAG→CAT yqeF ← short chain acyltransferase
RA 2,985,157 C→A 8.9% intergenic (+39/‑296) ygeG → / → ygeH SycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator
RA 3,006,103 C→A 19.1% R499L (CGT→CTT)  yqeB ← XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
RA 3,013,084 G→T 6.4% E189* (GAG→TAG)  ssnA → putative chlorohydrolase/aminohydrolase
RA 3,015,849 C→A 7.0% P392Q (CCG→CAG)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
RA 3,021,589 C→A 8.9% P370P (CCC→CCA ygfQ → putative purine permease
RA 3,064,718 T→G 21.7% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,065,273 C→A 7.5% D237Y (GAC→TAC)  pgk ← phosphoglycerate kinase
RA 3,071,761 G→T 12.8% P153Q (CCG→CAG)  cmtA ← putative mannitol‑specific PTS enzymes: IIB component/IIC component
RA 3,073,350 G→T 5.5% Q549K (CAG→AAG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,083,695 C→A 6.7% intergenic (+84/‑11) yggI → / → endA Zn‑dependent metalloprotease‑related protein/DNA‑specific endonuclease I
RA 3,088,806 C→A 6.5% P117Q (CCG→CAG)  yggS → UPF0001 family protein, PLP‑binding
RA 3,100,937 G→T 5.6% C526* (TGC→TGA speC ← ornithine decarboxylase, constitutive
RA 3,105,440 G→T 6.7% pseudogene (10/963 nt) yghE ← pseudogene, secretion pathway protein, L‑type protein homology; putative general secretion pathway for protein export (GSP)
RA 3,111,002 C→A 6.2% V490V (GTG→GTT yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,118,773 Δ1 bp 7.4% coding (46/1224 nt) glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
RA 3,118,774 Δ1 bp 7.4% coding (45/1224 nt) glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
RA 3,120,172 G→T 9.3% P403P (CCC→CCA glcD ← glycolate oxidase subunit, FAD‑linked
RA 3,125,638 C→A 8.9% G44C (GGT→TGT)  yghQ ← putative inner membrane polysaccharide flippase
RA 3,126,992 G→A 5.1% R109C (CGC→TGC)  yghS ← putative ATP‑binding protein
RA 3,130,437 G→T 7.6% P482Q (CCG→CAG)  gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase
RA 3,145,823 G→T 11.3% G77C (GGC→TGC)  metC → cystathionine beta‑lyase, PLP‑dependent
RA 3,192,205 C→A 7.4% D266Y (GAT→TAT)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,206,139 G→A 7.1% E558E (GAG→GAA dnaG → DNA primase
RA 3,206,241 G→T 5.1% intergenic (+30/‑165) dnaG → / → rpoD DNA primase/RNA polymerase, sigma 70 (sigma D) factor
RA 3,216,558 G→T 6.3% Q189H (CAG→CAT ebgA → evolved beta‑D‑galactosidase, alpha subunit
RA 3,273,750 C→T 9.5% R59C (CGT→TGT)  agaV → N‑acetylgalactosamine‑specific enzyme IIB component of PTS
RA 3,298,217 C→A 6.1% V320V (GTG→GTT mtr ← tryptophan transporter of high affinity
RA 3,307,639 C→A 7.4% G579C (GGT→TGT)  infB ← translation initiation factor IF‑2
RA 3,308,524 C→A 8.0% G284C (GGC→TGC)  infB ← translation initiation factor IF‑2
RA 3,317,714 G→T 8.5% P186H (CCC→CAC)  folP ← 7,8‑dihydropteroate synthase
RA 3,331,496 C→A 5.6% E109* (GAA→TAA)  mlaD ← ABC transporter maintaining OM lipid asymmetry, anchored periplasmic binding protein
RA 3,336,004 G→T 5.4% D125Y (GAT→TAT)  kdsC → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase
RA 3,346,586 C→A 7.0% W275L (TGG→TTG)  yhcC ← putative Fe‑S oxidoreductase, Radical SAM superfamily protein
RA 3,349,210 G→A 9.1% G376D (GGT→GAT)  gltB → glutamate synthase, large subunit
RA 3,350,612 C→A 9.1% V843V (GTC→GTA gltB → glutamate synthase, large subunit
RA 3,385,928 C→A 12.5% L1230L (CTG→CTT yhdP ← DUF3971‑AsmA2 domains protein
RA 3,395,730 G→A 5.4% A321V (GCA→GTA)  csrD ← targeting factor for csrBC sRNA degradation
RA 3,397,319 C→T 6.5% V159V (GTC→GTT acuI → putative acryloyl‑CoA reductase
RA 3,416,899 A→G 7.2% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,424,994 G→A 5.2% R110W (CGG→TGG)  yrdD ← putative DNA topoisomerase
RA 3,462,341 G→T 5.7% P291H (CCT→CAT)  chiA ← periplasmic endochitinase
RA 3,485,651 G→T 8.9% F2L (TTC→TTA ppiA ← peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
RA 3,523,042 G→T 5.5% P112P (CCG→CCT hslR → ribosome‑associated heat shock protein Hsp15
RA 3,537,916 G→T 5.9% P96P (CCC→CCA bioH ← pimeloyl‑ACP methyl ester carboxylesterase
RA 3,550,165 C→A 6.9% G15V (GGC→GTC)  rtcA ← RNA 3'‑terminal phosphate cyclase
RA 3,552,763 Δ1 bp 8.2% coding (1137/1599 nt) rtcR → sigma 54‑dependent transcriptional regulator of rtcBA expression
RA 3,559,474 G→T 18.2% F156L (TTC→TTA glgP ← glycogen phosphorylase
RA 3,581,713 C→A 15.5% M276I (ATG→ATT ugpC ← glycerol‑3‑phosphate transporter subunit
RA 3,596,692 C→A 13.8% V304V (GTG→GTT ftsY ← Signal Recognition Particle (SRP) receptor
RA 3,701,364 C→A 11.0% intergenic (‑299/+612) dppA ← / ← proK dipeptide transporter/tRNA‑Pro
RA 3,705,231 C→A 5.3% S46I (AGC→ATC)  yhjX ← pyruvate‑inducible inner membrane protein, putative transporter
RA 3,729,074 G→A 5.7% A246T (GCG→ACG)  xylR → xylose divergent operon transcriptional activator
RA 3,732,734 C→T 5.1% S626S (AGC→AGT malS → alpha‑amylase
RA 3,759,052 C→T 6.5% Y1170Y (TAC→TAT rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,759,248 T→C 5.1% L1236L (TTG→CTG)  rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,767,150 G→T 7.7% G504C (GGT→TGT)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
RA 3,772,935 G→A 5.3% L174L (CTG→CTA lldR → dual role activator/repressor for lldPRD operon
RA 3,824,326 C→A 7.8% A170A (GCC→GCA yicH → putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,827,542 G→T 6.3% S119* (TCA→TAA)  yicI ← putative alpha‑glucosidase
RA 3,877,923 A→G 90.2% Q24R (CAG→CGG)  rnpA → protein C5 component of RNase P
RA 3,879,272 G→T 6.4% L279L (CTG→CTT yidC → membrane protein insertase
RA 3,911,338 G→T 6.9% F96L (TTC→TTA atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit
RA 3,918,498 C→T 6.4% G166S (GGT→AGT)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,918,630:1 +G 7.0% coding (364/1890 nt) mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,918,630:2 +A 7.0% coding (364/1890 nt) mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,918,630:3 +A 7.0% coding (364/1890 nt) mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,918,630:4 +G 7.0% coding (364/1890 nt) mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,918,630:5 +A 7.0% coding (364/1890 nt) mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,923,871 C→A 12.0% D195Y (GAT→TAT)  ravA ← hexameric AAA+ MoxR family ATPase, putative molecular chaperone
RA 3,935,371 G→T 5.9% noncoding (204/1542 nt) rrsC → 16S ribosomal RNA of rrnC operon
RA 3,936,724 T→A 6.9% intergenic (+15/‑71) rrsC → / → gltU 16S ribosomal RNA of rrnC operon/tRNA‑Glu
RA 3,977,554 A→G 6.2% intergenic (+1/+158) aslB → / ← aslA putative AslA‑specific sulfatase‑maturating enzyme/putative Ser‑type periplasmic non‑aryl sulfatase
RA 3,993,815 Δ1 bp 8.2% coding (602/765 nt) yigE ← DUF2233 family protein
RA 3,993,816 Δ1 bp 8.0% coding (601/765 nt) yigE ← DUF2233 family protein
RA 4,004,549 G→T 6.6% L38L (CTG→CTT yigM → putative inner membrane EamA‑like transporter
RA 4,019,993 Δ1 bp 75.7% coding (107/702 nt) fre → NAD(P)H‑flavin reductase
RA 4,024,042 C→T 5.3% R97H (CGC→CAC)  fadB ← fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase
RA 4,037,133 A→G 8.7% Q119R (CAG→CGG)  dsbA → periplasmic protein disulfide isomerase I
RA 4,037,217 G→T 11.1% S147I (AGC→ATC)  dsbA → periplasmic protein disulfide isomerase I
RA 4,051,827 C→A 8.9% L21M (CTG→ATG)  typA → GTP‑binding protein
RA 4,075,821 C→T 7.4% C100Y (TGT→TAT)  fdoH ← formate dehydrogenase‑O, Fe‑S subunit
RA 4,096,194 G→A 5.7% W149* (TGG→TGA cpxP → inhibitor of the cpx response; periplasmic adaptor protein
RA 4,104,821 G→A 9.2% N229N (AAC→AAT glpX ← fructose 1,6‑bisphosphatase II
RA 4,125,115 C→A 6.3% I451I (ATC→ATA katG → catalase‑peroxidase HPI, heme b‑containing
RA 4,131,966 C→T 5.3% D62N (GAT→AAT)  frwA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
RA 4,140,442 G→T 5.3% P862H (CCT→CAT)  ppc ← phosphoenolpyruvate carboxylase
RA 4,140,445:1 +A 5.3% coding (2582/2652 nt) ppc ← phosphoenolpyruvate carboxylase
RA 4,140,445:2 +C 5.3% coding (2582/2652 nt) ppc ← phosphoenolpyruvate carboxylase
RA 4,140,445:3 +G 5.3% coding (2582/2652 nt) ppc ← phosphoenolpyruvate carboxylase
RA 4,140,448:1 +C 5.1% coding (2579/2652 nt) ppc ← phosphoenolpyruvate carboxylase
RA 4,140,448:2 +A 5.1% coding (2579/2652 nt) ppc ← phosphoenolpyruvate carboxylase
RA 4,140,450 T→G 5.1% E859D (GAA→GAC) ‡ ppc ← phosphoenolpyruvate carboxylase
RA 4,140,451 T→C 5.1% E859G (GAA→GGA) ‡ ppc ← phosphoenolpyruvate carboxylase
RA 4,140,452 C→G 5.1% E859Q (GAA→CAA) ‡ ppc ← phosphoenolpyruvate carboxylase
RA 4,165,449 C→T 18.9% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 18.9% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,165,460 G→T 5.2% noncoding (61/85 nt) tyrU → tRNA‑Tyr
RA 4,166,006 C→T 12.0% R45R (CGC→CGT tufB → translation elongation factor EF‑Tu 2
RA 4,177,586 T→C 88.9% L770P (CTC→CCC)  rpoC → RNA polymerase, beta prime subunit
RA 4,180,725 C→A 13.1% G358C (GGT→TGT)  thiH ← tyrosine lyase, involved in thiamine‑thiazole moiety synthesis
RA 4,196,303 C→T 8.3% L386L (CTG→CTA purH ← fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase
RA 4,202,979 A→C 26.3% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,218,959 G→T 11.2% R434L (CGG→CTG)  yjbB → putative Na+/Pi‑cotransporter
RA 4,223,539 G→T 5.7% intergenic (‑378/‑147) lysC ← / → pgi lysine‑sensitive aspartokinase 3/glucosephosphate isomerase
RA 4,223,964 C→A 7.2% N93K (AAC→AAA pgi → glucosephosphate isomerase
RA 4,257,146 G→T 10.9% L35L (CTG→CTT tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
RA 4,266,568 T→C 6.8% I390I (ATT→ATC yjcC → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 4,269,394 G→T 5.8% G330C (GGC→TGC)  yjcD → inner membrane putative guanine permease
RA 4,279,429 C→A 5.2% S86* (TCA→TAA)  nrfB → nitrite reductase, formate‑dependent, penta‑heme cytochrome c
RA 4,296,235 G→A 7.4% D60D (GAC→GAT yjcS ← metallo‑beta‑lactamase superfamily protein
RA 4,305,988 C→A 5.9% G350C (GGC→TGC)  phnM ← ribophosphonate triphosphate hydrolase
RA 4,315,905 T→G 7.6% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,938 C→T 7.0% intergenic (‑380/+278) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,939 A→C 5.8% intergenic (‑381/+277) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,951 A→T 6.3% intergenic (‑393/+265) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,956 Δ1 bp 6.3% intergenic (‑398/+260) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,959 G→T 6.4% intergenic (‑401/+257) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,965:1 +A 6.1% intergenic (‑407/+251) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,966 T→A 6.1% intergenic (‑408/+250) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,316,005 G→T 7.2% intergenic (‑447/+211) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,340,336 G→T 9.1% Q382K (CAG→AAG)  dcuS ← sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
RA 4,362,494 C→A 10.9% intergenic (+6/‑132) groL → / → yjeI Cpn60 chaperonin GroEL, large subunit of GroESL/DUF4156 family lipoprotein
RA 4,363,098 T→C 5.4% intergenic (+119/+84) yjeI → / ← yjeJ DUF4156 family lipoprotein/uncharacterized protein
RA 4,398,431 T→G 8.9% V654G (GTC→GGC) ‡ rnr → exoribonuclease R, RNase R
RA 4,398,432 C→G 9.3% V654V (GTC→GTG) ‡ rnr → exoribonuclease R, RNase R
RA 4,398,435 Δ1 bp 9.4% coding (1965/2442 nt) rnr → exoribonuclease R, RNase R
RA 4,398,436 Δ1 bp 9.4% coding (1966/2442 nt) rnr → exoribonuclease R, RNase R
RA 4,398,437 Δ1 bp 9.4% coding (1967/2442 nt) rnr → exoribonuclease R, RNase R
RA 4,398,438 C→A 9.4% G656G (GGC→GGA rnr → exoribonuclease R, RNase R
RA 4,398,440 T→A 9.4% F657Y (TTT→TAT) ‡ rnr → exoribonuclease R, RNase R
RA 4,398,441 T→G 9.4% F657L (TTT→TTG) ‡ rnr → exoribonuclease R, RNase R
RA 4,398,442 G→C 9.4% G658R (GGC→CGC) ‡ rnr → exoribonuclease R, RNase R
RA 4,398,443 G→C 9.2% G658A (GGC→GCC) ‡ rnr → exoribonuclease R, RNase R
RA 4,399,668 C→T 8.9% L193L (CTG→TTG)  rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,402,207 G→T 8.6% A2S (GCC→TCC)  yjfM → DUF1190 family protein
RA 4,431,948 G→T 8.2% L16M (CTG→ATG)  msrA ← methionine sulfoxide reductase A
RA 4,437,157 G→T 13.4% A1077S (GCC→TCC)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,468,398 C→A 8.7% H37N (CAT→AAT)  rraB → protein inhibitor of RNase E
RA 4,486,551 G→T 5.8% pseudogene (60/1266 nt) intB → pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase
RA 4,498,981 G→A 7.0% pseudogene (156/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,499,008 C→T 6.2% pseudogene (183/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,499,011 T→C 5.4% pseudogene (186/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,444 C→A 5.3% P34H (CCT→CAT) ‡ insA → IS1 repressor TnpA
RA 4,508,445 T→C 5.4% P34P (CCT→CCC) ‡ insA → IS1 repressor TnpA
RA 4,508,466 A→G 5.5% L41L (CTA→CTG insA → IS1 repressor TnpA
RA 4,508,484 C→T 8.6% A47A (GCC→GCT insA → IS1 repressor TnpA
RA 4,508,490 G→A 9.1% Q49Q (CAG→CAA insA → IS1 repressor TnpA
RA 4,508,498 A→C 7.1% K52T (AAG→ACG)  insA → IS1 repressor TnpA
RA 4,514,221 G→A 9.5% L203L (CTG→TTG)  yjhH ← putative lyase/synthase
RA 4,523,165 G→T 6.8% P146Q (CCG→CAG)  yjhQ ← putative acetyltransferase
RA 4,534,041 G→A 6.0% G166D (GGC→GAC)  fimI → fimbrial protein involved in type 1 pilus biosynthesis
RA 4,538,741 C→A 5.3% A39A (GCC→GCA fimH → minor component of type 1 fimbriae
RA 4,560,372 G→T 10.9% I340I (ATC→ATA yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase
RA 4,571,155 G→T 8.4% P42H (CCT→CAT)  hsdS ← specificity determinant for hsdM and hsdR
RA 4,599,641 C→A 5.4% I137I (ATC→ATA prfC → peptide chain release factor RF‑3

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 463819 464287 469 10 [9] [9] 11 [ybaO] [ybaO]
* * ÷ CP009273 2027284 2027418 135 10 [8] [7] 10 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein
* * ÷ CP009273 2622923 2623068 146 10 [8] [9] 13 yfgG/yfgH uncharacterized protein/outer membrane integrity lipoprotein
* * ÷ CP009273 3448791 3448865 75 10 [8] [2] 11 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ CP009273 3792511 3792780 270 11 [8] [8] 10 [waaU]–[waaZ] [waaU],[waaZ]
* * ÷ CP009273 3797648 3797831 184 14 [9] [6] 11 waaS lipopolysaccharide core biosynthesis protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 33571765 (1.120)6 (0.130)
+29 bp
3/188 NT 10.7% noncoding (250/358 nt) REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences
?CP009273 = 372985 NA (NA)intergenic (+218/+6) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 360104 =55 (0.940)55 (0.940) 21/248 NT 50.0% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 37038586 (2.020)16 (0.420) 7/162 NT 17.1% intergenic (+48/‑530) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 = 370419119 (2.030)7 (0.120) 3/240 NT 6.2% noncoding (34/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 = 372843 97 (1.710)noncoding (53/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 372944 =55 (0.940)26 (0.520) 5/210 NT 34.3% noncoding (154/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
?CP009273 4315902 = 53 (1.070)noncoding (284/583 nt) REP325 (repetitive extragenic palindromic) element; contains 12 REP sequences REP325 (repetitive extragenic palindromic) element; contains 12 REP sequences
* ? CP009273 = 627604NA (NA)3 (0.070) 3/176 NT 5.8% noncoding (34/34 nt) REP55 (repetitive extragenic palindromic) element; contains 1 REP sequences REP55 (repetitive extragenic palindromic) element; contains 1 REP sequences
?CP009273 = 4398969 49 (1.160)intergenic (+57/‑123) rnr/rlmB exoribonuclease R, RNase R/23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
* ? CP009273 = 104600188 (1.500)48 (0.820) 19/248 NT 49.7% intergenic (+15/+418) insB1/cspH IS1 transposase B/stress protein, member of the CspA‑family
?CP009273 = 1972777 9 (0.150)intergenic (‑323/‑457) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1203246 =20 (0.340)44 (0.860) 20/216 NT 73.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 15 (0.290)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326118 (0.310)32 (0.630) 15/216 NT 67.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 15 (0.290)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1972769 =4 (0.070)46 (0.790) 26/246 NT 92.1% intergenic (‑315/‑465) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA