breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 4,613 C→A 7.2% Q294K (CAG→AAG)  thrC → L‑threonine synthase
RA 35,227:4 +G 11.3% coding (145/591 nt) caiE ← stimulator of CaiD and CaiB enzyme activities
RA 35,227:5 +T 11.3% coding (145/591 nt) caiE ← stimulator of CaiD and CaiB enzyme activities
RA 35,227:6 +A 11.3% coding (145/591 nt) caiE ← stimulator of CaiD and CaiB enzyme activities
RA 35,227:7 +G 11.3% coding (145/591 nt) caiE ← stimulator of CaiD and CaiB enzyme activities
RA 35,227:8 +T 11.3% coding (145/591 nt) caiE ← stimulator of CaiD and CaiB enzyme activities
RA 35,227:10 +A 11.3% coding (145/591 nt) caiE ← stimulator of CaiD and CaiB enzyme activities
RA 89,348 G→T 8.4% K483N (AAG→AAT ftsI → transpeptidase involved in septal peptidoglycan synthesis (penicillin‑binding protein 3)
RA 158,283 G→T 6.3% A79D (GCC→GAC)  ligT ← 2'‑5' RNA ligase
RA 187,742 C→T 20.0% R133R (CGC→CGT tsf → translation elongation factor EF‑Ts
RA 221,373:1 +G 11.7% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 11.9% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 12.2% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 12.2% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 12.5% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 12.5% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 12.5% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 12.8% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 12.5% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 13.1% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 13.5% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 14.1% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 17.8% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 17.9% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 19.7% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 235,769 G→T 12.8% pseudogene (177/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 243,088 C→A 18.2% intergenic (‑99/‑111) dinJ ← / → yafL antitoxin of YafQ‑DinJ toxin‑antitoxin system/putative lipoprotein and C40 family peptidase
RA 252,656 C→A 7.3% H65N (CAC→AAC)  gpt → xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase
RA 262,559 C→T 5.0% pseudogene (120/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 302,895 G→T 7.3% L717I (CTC→ATC)  ecpC ← ECP production outer membrane protein
RA 330,901 G→T 5.5% intergenic (‑168/‑90) yahC ← / → yahD putative inner membrane protein/ankyrin repeat protein
RA 335,511 A→G 7.1% intergenic (+57/‑365) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 353,286 T→A 8.5% intergenic (+121/+216) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 353,289 T→C 8.9% intergenic (+124/+213) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
JC JC 370,378 REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences (–) +8 bp :: +ATCTGC 16.2% intergenic (+41/‑530) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,400 G→A 5.7% intergenic (+63/‑515) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,428 T→C 8.7% intergenic (+91/‑487) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,430 A→G 7.4% intergenic (+93/‑485) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 370,442 T→C 8.6% intergenic (+105/‑473) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 431,498 T→A 6.9% F137I (TTT→ATT)  thiL → thiamine monophosphate kinase
RA 517,301 T→C 7.3% L477P (CTG→CCG)  ybbP → putative ABC transporter permease
RA 541,682 C→T 5.9% A46A (GCG→GCA allD ← ureidoglycolate dehydrogenase
RA 561,445 C→A 7.6% pseudogene (91/222 nt) xisD ← pseudogene, exisionase in defective prophage DLP12;Phage or Prophage Related
RA 572,881 G→A 8.9% G10S (GGT→AGT)  essD → DLP12 prophage; putative phage lysis protein
RA 633,718 G→A 5.9% S104S (TCC→TCT dsbG ← thiol:disulfide interchange protein, periplasmic
RA 730,479 C→G 9.5% pseudogene (891/1521 nt) rhsO → pseudogene, Rhs family protein
RA 733,913 T→C 8.3% R122R (CGT→CGC ybfD → H repeat‑associated putative transposase
RA 733,915 A→G 8.4% K123R (AAG→AGG)  ybfD → H repeat‑associated putative transposase
RA 791,167 C→A 13.3% A208D (GCC→GAC)  modA → molybdate transporter subunit
RA 798,822 C→T 10.5% intergenic (+46/‑137) ybhI → / → ybhJ putative transporter/putative hydratase
RA 802,072 G→T 5.1% S222R (AGC→AGA ybhC ← acyl‑CoA thioesterase, lipoprotein
RA 831,364 C→T 7.5% R221W (CGG→TGG)  ybiB → putative family 3 glycosyltransferase
RA 843,451 C→A 8.5% V4L (GTA→TTA)  glnH ← glutamine transporter subunit
RA 857,367 C→A 7.9% A232A (GCG→GCT ybiW ← putative pyruvate formate lyase
RA 893,162 C→A 7.0% S208* (TCG→TAG)  potI → putrescine transporter subunit: membrane component of ABC superfamily
RA 923,518 G→A 6.1% G378G (GGC→GGT cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 931,070 G→A 14.8% P797P (CCG→CCA ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 931,074 G→C 15.4% A799P (GCG→CCG)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 931,084 A→C 15.5% Q802P (CAG→CCG)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 934,753 C→T 6.6% A435V (GCT→GTT)  rarA → DNA‑dependent ATPase involved in processing recombination intermediates at replication forks
RA 935,695 C→A 8.2% A271D (GCC→GAC)  serS → seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine
RA 986,854 T→C 7.5% N259N (AAT→AAC pepN → aminopeptidase N
RA 997,974 G→A 5.5% A238T (GCT→ACT)  elfG → putative fimbrial‑like adhesin protein
RA 1,049,974 G→A 9.9% A554V (GCG→GTG)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,124,559 C→T 6.0% A422V (GCT→GTT)  murJ → putative peptidoglycan lipid II flippase
RA 1,150,377 G→A 6.6% A270A (GCG→GCA yceG → septation protein, ampicillin sensitivity
RA 1,162,039 G→T 6.3% A167S (GCG→TCG)  ndh → respiratory NADH dehydrogenase 2/cupric reductase
RA 1,216,968 G→T 6.2% pseudogene (1912/2648 nt) ycgH → pseudogene; putative ATP‑binding component of a transport system
RA 1,249,740 C→A 5.4% A557S (GCT→TCT)  ycgV ← putative adhesin
RA 1,255,342 C→A 11.1% T306T (ACG→ACT dauA ← C4‑dicarboxylic acid transporter
RA 1,282,249 A→G 8.2% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,608 T→C 5.9% noncoding (85/171 nt)
T9A (ACT→GCT) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,386,783 G→A 10.3% S179N (AGT→AAT)  pgrR → murein peptide degradation regulator
RA 1,422,060 G→C 10.5% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,132 C→T 6.6% pseudogene (852/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,150 C→T 6.6% pseudogene (834/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 5.9% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,435,752 G→T 5.8% A83A (GCC→GCA hslJ ← heat‑inducible lipoprotein involved in novobiocin resistance
RA 1,460,567 A→G 7.5% pseudogene (919/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,593 C→G 8.1% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,602 T→C 10.7% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,752 C→T 6.2% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,896 C→T 10.9% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,461,115 T→C 6.8% pseudogene (1467/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,513,989 C→T 5.3% P236S (CCG→TCG)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,522,243 C→T 5.2% pseudogene (85/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,534,579 G→A 5.9% Q757* (CAA→TAA)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,537,305 G→T 11.8% P338Q (CCG→CAG)  narU ← nitrate/nitrite transporter
RA 1,565,668 G→A 6.9% T212I (ACC→ATC)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,583,241 C→A 10.3% pseudogene (1096/1227 nt) ydeT ← pseudogene; putative outer membrane protein
RA 1,593,036 G→A 9.1% T477T (ACC→ACT lsrK ← autoinducer‑2 (AI‑2) kinase
RA 1,594,117 G→A 15.6% A117V (GCA→GTA)  lsrK ← autoinducer‑2 (AI‑2) kinase
RA 1,629,745 T→A 7.2% A120A (GCA→GCT stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,629,762 T→C 8.5% T115A (ACT→GCT)  stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,673,066 G→T 10.0% G128V (GGC→GTC)  ydgH → DUF1471 family periplasmic protein
RA 1,679,621 C→T 12.9% V409M (GTG→ATG)  fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,784,034 G→T 8.5% Q55H (CAG→CAT ydiE → hemin uptake protein HemP homolog
RA 1,785,755 G→T 9.3% H175N (CAC→AAC)  ydiV ← anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase
RA 1,912,600 G→T 7.4% Q278H (CAG→CAT yebT → MCE domain protein
RA 1,928,790 G→T 5.1% L24L (CTC→CTA edd ← 6‑phosphogluconate dehydratase
RA 2,033,194 C→T 10.5% T79M (ACG→ATG)  yedY → membrane‑anchored, periplasmic TMAO, DMSO reductase
RA 2,109,742 G→A 9.3% S289S (AGC→AGT wcaL ← putative glycosyl transferase
RA 2,217,814 G→T 6.2% T179N (ACC→AAC)  pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,224,736 A→G 7.1% Q80R (CAG→CGG)  yohK → LrgB family inner membrane protein
RA 2,298,492 A→G 6.8% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,337,310 C→A 12.5% G113G (GGG→GGT yfaL ← adhesin
RA 2,341,366 G→A 5.1% W168* (TGG→TGA nrdB → ribonucleoside‑diphosphate reductase 1, beta subunit, ferritin‑like protein
RA 2,365,166 C→T 7.5% Y260Y (TAC→TAT arnT → 4‑amino‑4‑deoxy‑L‑arabinose transferase
RA 2,367,106 A→G 8.0% intergenic (‑89/+21) pmrD ← / ← menE inactive two‑component system connector protein/O‑succinylbenzoate‑CoA ligase
RA 2,399,955 C→A 100% E56* (GAA→TAA)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,437,163 A→G 7.1% M641V (ATG→GTG)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,437,724 Δ1 bp 7.4% coding (507/549 nt) epmC ← Elongation Factor P Lys34 hydroxylase
RA 2,481,148 T→C 7.7% L1099P (CTA→CCA)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 2,499,919 C→A 6.0% G65C (GGC→TGC)  ypdF ← Xaa‑Pro aminopeptidase
RA 2,518,005 G→T 5.7% P78Q (CCG→CAG)  xapA ← purine nucleoside phosphorylase II
RA 2,535,092 Δ1 bp 9.1% coding (780/834 nt) cysU ← sulfate/thiosulfate ABC transporter permease
RA 2,535,093 Δ1 bp 9.1% coding (779/834 nt) cysU ← sulfate/thiosulfate ABC transporter permease
RA 2,535,094 Δ1 bp 9.1% coding (778/834 nt) cysU ← sulfate/thiosulfate ABC transporter permease
RA 2,537,033 T→G 5.4% intergenic (‑146/+158) cysP ← / ← ucpA thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase
RA 2,550,583 G→T 8.2% S25* (TCA→TAA)  eutC ← ethanolamine ammonia‑lyase, small subunit (light chain)
RA 2,607,874 G→T 6.3% M194I (ATG→ATT focB → putative formate transporter
RA 2,677,576 G→A 5.5% intergenic (+161/+37) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,678,532 G→A 18.2% P112L (CCA→CTA)  glyA ← serine hydroxymethyltransferase
RA 2,703,152 G→A 10.5% L74L (CTG→TTG)  rpoE ← RNA polymerase sigma E factor
RA 2,714,813 G→T 6.3% W501L (TGG→TTG)  pka → protein lysine acetyltransferase
RA 2,719,536 G→T 24.3% noncoding (12/120 nt) rrfG ← 5S ribosomal RNA of rrnG operon
RA 2,739,962 C→T 7.7% R398H (CGT→CAT)  ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
RA 2,762,984 T→G 7.5% intergenic (+139/‑78) yfjQ → / → yfjR CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,806,217 G→T 8.4% L81L (CTG→CTT emrB → multidrug efflux system protein
RA 2,811,275 T→G 17.3% intergenic (‑56/+143) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,814,979 G→A 9.2% T131I (ACC→ATC)  alaS ← alanyl‑tRNA synthetase
RA 2,843,524 G→A 5.2% R91W (CGG→TGG)  hycA ← regulator of the transcriptional regulator FhlA
RA 2,928,793 C→A 5.1% V400V (GTC→GTA fucP → L‑fucose transporter
RA 2,929,562 C→A 11.9% A207E (GCG→GAG)  fucI → L‑fucose isomerase
RA 3,037,040 C→A 6.3% T7N (ACC→AAC)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,040,197 G→A 6.2% P735L (CCG→CTG)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
RA 3,063,116 C→A 5.6% L17L (CTG→CTT mscS ← mechanosensitive channel protein, small conductance
RA 3,087,812 G→A 7.5% L210L (CTG→TTG)  yggR ← putative PilT family AAA+ ATPase
RA 3,107,243 C→A 6.7% V157L (GTA→TTA)  pppA ← bifunctional prepilin leader peptidase/ methylase
RA 3,128,961 C→T 7.0% G257D (GGC→GAC)  pitB ← phosphate transporter
RA 3,267,478 G→T 5.0% N263K (AAC→AAA garP ← putative (D)‑galactarate transporter
RA 3,416,810 G→A 7.4% noncoding (92/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,890 T→G 10.5% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,448,174 C→T 5.6% R195H (CGC→CAC)  gspA ← general secretory pathway component, cryptic
RA 3,464,590 G→A 5.3% T33T (ACC→ACT tufA ← translation elongation factor EF‑Tu 1
RA 3,493,486 A→T 6.7% N73I (AAT→ATT)  frlA → putative fructoselysine transporter
RA 3,546,241 G→T 5.8% intergenic (‑409/‑203) malP ← / → malT maltodextrin phosphorylase/mal regulon transcriptional activator
RA 3,548,003 G→A 5.6% Q520Q (CAG→CAA malT → mal regulon transcriptional activator
RA 3,590,467 C→T 5.1% A152T (GCA→ACA)  livK ← leucine transporter subunit
RA 3,607,907 C→A 14.1% A294E (GCG→GAG)  nikA → nickel‑binding, heme‑binding periplasmic protein
RA 3,608,854 C→A 5.1% A85D (GCC→GAC)  nikB → nickel transporter subunit
RA 3,618,205 A→T 16.0% P156P (CCA→CCT yhhI → putative transposase
RA 3,671,762 G→T 12.9% intergenic (‑52/+18) yhjG ← / ← yhjH putative inner membrane‑anchored periplasmic AsmA family protein/cyclic‑di‑GMP phosphodiesterase, FlhDC‑regulated
RA 3,690,677 G→A 5.6% R287H (CGT→CAT)  bcsE → cellulose production protein
RA 3,703,793 A→G 7.6% L15L (TTG→CTG)  eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible
RA 3,707,152 C→A 10.6% N47K (AAC→AAA yiaC → putative acyl‑CoA transferase
RA 3,711,059 G→T 7.2% W130C (TGG→TGT ghrB → glyoxylate/hydroxypyruvate reductase B
RA 3,788,171 C→T 7.8% F275F (TTC→TTT hldD → ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
RA 3,851,607 G→T 7.3% T53K (ACG→AAG)  yidJ ← sulfatase/phosphatase superfamily protein
RA 3,869,526 C→A 6.1% A263S (GCT→TCT)  yidA ← sugar phosphate phosphatase; substrates include erythrose 4‑P and mannose 1‑P phosphatase
RA 3,922,352 T→C 7.8% E204E (GAA→GAG viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein
RA 3,932,260 G→A 12.1% G225E (GGG→GAG)  rbsR → transcriptional repressor of ribose metabolism
RA 3,936,724 T→A 8.7% intergenic (+15/‑71) rrsC → / → gltU 16S ribosomal RNA of rrnC operon/tRNA‑Glu
RA 3,944,266 G→A 7.3% pseudogene (347/984 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
RA 4,001,021 Δ1 bp 6.1% coding (1798/1830 nt) recQ → ATP‑dependent DNA helicase
RA 4,076,588 T→C 6.4% P865P (CCA→CCG fdoG ← formate dehydrogenase‑O, large subunit
RA 4,143,375 A→G 6.1% intergenic (‑349/+249) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,165,449 C→T 18.7% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 17.1% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,165,886 G→A 11.7% K5K (AAG→AAA tufB → translation elongation factor EF‑Tu 2
RA 4,176,469 A→G 81.0% K398E (AAG→GAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,210,112 C→A 8.6% P530Q (CCG→CAG)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,221,292 G→A 6.3% R90C (CGC→TGC)  yjbD ← DUF3811 family protein
RA 4,228,029 G→T 7.9% L156L (CTG→CTT yjbH → DUF940 family extracellular polysaccharide protein
RA 4,231,860 C→T 6.6% G108D (GGT→GAT)  xylE ← D‑xylose transporter
RA 4,239,280 G→A 9.7% intergenic (+41/‑202) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,336,205 G→A 7.3% G313G (GGC→GGT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,366,743 G→A 10.0% V18I (GTT→ATT)  sugE → multidrug efflux system protein
RA 4,370,464 C→T 5.7% A558T (GCT→ACT)  frdA ← fumarate reductase (anaerobic) catalytic and NAD/flavoprotein subunit
RA 4,375,030 G→T 6.4% E459* (GAA→TAA)  yjeM → putative transporter
RA 4,376,463 C→T 8.5% V909I (GTT→ATT)  mscM ← mechanosensitive channel protein, miniconductance
RA 4,415,412 C→A 5.5% H26N (CAC→AAC)  priB → primosomal protein N
RA 4,573,155 C→A 15.1% pseudogene (3478/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,597,545 C→T 5.1% intergenic (‑28/‑75) rsmC ← / → holD 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent/DNA polymerase III, psi subunit
RA 4,614,692 A→T 5.5% intergenic (‑86/‑20) ytjB ← / → serB SMP_2 family putative membrane‑anchored periplasmic protein/3‑phosphoserine phosphatase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 578881 579074 194 7 [6] [5] 7 tfaX/appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
* * ÷ CP009273 1459352 1459482 131 10 [6] [5] 8 [ynbG] [ynbG]
* * ÷ CP009273 2027304 2027428 125 8 [6] [6] 10 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein
* * ÷ CP009273 2208334 2209051 718 7 [5] [5] 7 [mlrA] [mlrA]
* * ÷ CP009273 2462825 2463038 214 7 [4] [6] 7 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2988477 2988711 235 7 [6] [6] 11 [ygeN] [ygeN]
* * ÷ CP009273 3792470 3792706 237 8 [6] [5] 7 [waaU]–[waaZ] [waaU],[waaZ]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =37 (0.640)53 (0.910) 25/248 NT 58.9% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370386 =NA (NA)24 (0.630) 8/162 NT 32.7% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372978 = 55 (1.310)intergenic (+211/+13) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 = 60346655 (0.950)8 (0.150) 3/224 NT 10.3% intergenic (+22/‑55) hokE/insL1 toxic polypeptide, small/IS186 transposase
?CP009273 604816 = 89 (1.700)intergenic (+183/+99) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? CP009273 1203246 =22 (0.380)16 (0.320) 13/216 NT 51.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 11 (0.220)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326120 (0.350)18 (0.360) 10/216 NT 55.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 11 (0.220)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related