breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 36,137 C→A 7.6% R9L (CGC→CTC)  caiD ← carnitinyl‑CoA dehydratase
RA 39,484 C→A 8.1% A301A (GCG→GCT caiA ← crotonobetaine reductase subunit II, FAD‑binding
RA 59,116 C→T 8.6% pseudogene (65/228 nt) yabP → pseudogene, pentapeptide repeats‑containing
RA 93,700 G→A 5.4% G43S (GGC→AGC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
RA 140,252 C→A 7.7% R22S (CGT→AGT)  yadH → inner membrane putative ABC superfamily transporter permease
RA 158,081 C→A 5.2% S146S (TCG→TCT ligT ← 2'‑5' RNA ligase
RA 193,172 C→A 13.3% A47E (GCG→GAG)  rseP → inner membrane zinc RIP metalloprotease; RpoE activator, by degrading RseA; cleaved signal peptide endoprotease
RA 208,073 G→A 6.1% A636A (GCG→GCA ldcC → lysine decarboxylase 2, constitutive
RA 221,163 A→G 5.3% noncoding (906/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,373:1 +G 6.7% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 7.0% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 7.0% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 7.0% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 7.1% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 7.2% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 7.1% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 7.1% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 7.1% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 7.3% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 7.5% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 7.7% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 12.6% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 12.9% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 12.5% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 305,989 G→T 5.1% L132M (CTG→ATG)  ecpA ← ECP pilin
RA 335,782 A→G 6.5% intergenic (+328/‑94) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 335,796:1 +T 7.2% intergenic (+342/‑80) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 345,512 C→T 5.4% intergenic (+229/‑211) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
JC JC 370,378 REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences (–) +8 bp :: +ATCTGC 11.0% intergenic (+41/‑530) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 372,807 Δ1 bp 16.4% intergenic (+40/+184) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,808 G→A 16.1% intergenic (+41/+183) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 470,547 C→A 7.7% G24V (GGC→GTC)  tesB ← acyl‑CoA thioesterase 2
RA 492,656 C→A 8.2% P9Q (CCG→CAG)  adk → adenylate kinase
RA 573,721 C→G 17.0% D53E (GAC→GAG rzpD → DLP12 prophage; putative murein endopeptidase
RA 605,631 Δ1 bp 5.4% intergenic (‑96/+79) entD ← / ← fepA phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter
RA 664,017 A→T 9.5% I52K (ATA→AAA)  rlmH ← 23S rRNA m(3)Psi1915 pseudouridine methyltransferase, SAM‑dependent
RA 686,512 C→A 6.6% V243V (GTG→GTT ybeX ← putative ion transport
RA 727,315 T→C 5.9% H759H (CAT→CAC rhsC → Rhs family putative polymorphic toxin
RA 727,325 A→G 6.2% N763D (AAT→GAT) ‡ rhsC → Rhs family putative polymorphic toxin
RA 727,327 T→A 6.0% N763K (AAT→AAA) ‡ rhsC → Rhs family putative polymorphic toxin
RA 727,329 A→C 6.4% E764A (GAA→GCA)  rhsC → Rhs family putative polymorphic toxin
RA 727,369 A→G 5.9% A777A (GCA→GCG rhsC → Rhs family putative polymorphic toxin
RA 727,375 A→G 5.8% G779G (GGA→GGG rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 6.7% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 728,467 T→C 5.4% I1143I (ATT→ATC rhsC → Rhs family putative polymorphic toxin
RA 730,083 G→A 12.2% pseudogene (495/1521 nt) rhsO → pseudogene, Rhs family protein
RA 733,602 G→A 6.1% A19T (GCC→ACC)  ybfD → H repeat‑associated putative transposase
RA 736,248 A→G 5.2% K429R (AAA→AGA)  phr → deoxyribodipyrimidine photolyase, FAD‑binding
RA 746,597 G→A 10.5% R343R (CGC→CGT ybgQ ← putative outer membrane protein
RA 775,713 G→A 6.0% M220I (ATG→ATA ybgF → periplasmic TolA‑binding protein
RA 777,208 C→A 11.3% intergenic (+100/‑333) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 783,865 G→A 10.8% G143G (GGC→GGT galM ← aldose 1‑epimerase; type‑1 mutarotase
RA 815,593 C→A 8.5% T85K (ACG→AAG)  ybhL → UPF0005 family inner membrane protein
RA 821,705 C→T 8.8% S335N (AGC→AAC)  ybhS ← inner membrane putative ABC superfamily transporter permease
RA 849,600 G→T 5.5% L166L (CTG→CTT ybiR → putative transporter
RA 910,671 T→G 10.7% intergenic (‑358/+137) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 973,498:1 +C 5.2% coding (1717/4461 nt) mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 975,954 C→A 8.0% R1391R (CGC→CGA mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 985,161 G→A 6.6% Q218* (CAG→TAG)  pncB ← nicotinate phosphoribosyltransferase
RA 1,013,783 A→T 8.7% intergenic (‑28/‑158) ycbZ ← / → matP putative peptidase/Ter macrodomain organizer matS‑binding protein
RA 1,040,738 G→T 6.2% Y174* (TAC→TAA gfcE ← putative O‑antigen capsule outer membrane auxillary protein export channel
RA 1,084,555 G→T 18.1% G253G (GGC→GGA pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
RA 1,095,551 G→A 6.1% E152E (GAG→GAA ycdY → redox enzyme maturation protein (REMP) chaperone for YcdX
RA 1,145,508 G→T 7.8% D109Y (GAT→TAT)  fabD → malonyl‑CoA‑[acyl‑carrier‑protein] transacylase
RA 1,169,957 A→G 11.3% L222P (CTG→CCG)  ycfT ← inner membrane protein
RA 1,203,100 C→T 5.3% C48C (TGC→TGT stfP → e14 prophage; uncharacterized protein
RA 1,242,981 G→T 9.5% intergenic (‑149/+171) treA ← / ← dhaM periplasmic trehalase/putative dihydroxyacetone‑specific PTS enzymes: HPr component/EI component
RA 1,250,094 G→T 5.2% R439S (CGT→AGT)  ycgV ← putative adhesin
RA 1,264,818 G→T 9.8% noncoding (40/67 nt) rdlA → sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA
RA 1,264,932 T→G 6.4% intergenic (+87/+227) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
RA 1,265,888 T→G 8.3% noncoding (40/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,282,249 A→G 11.5% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 5.8% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,284,178 G→T 8.0% P137P (CCC→CCA ychJ ← UPF0225 family protein
RA 1,306,817 G→T 11.4% L111M (CTG→ATG)  yciB ← IspA family inner membrane protein
RA 1,330,520 A→G 7.7% N145D (AAC→GAC)  acnA → aconitate hydratase 1
RA 1,351,147 G→A 11.4% P74L (CCC→CTC)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein
RA 1,353,843 G→T 9.6% F441L (TTC→TTA puuA ← glutamate‑‑putrescine ligase
RA 1,354,630 C→T 10.5% C179Y (TGC→TAC)  puuA ← glutamate‑‑putrescine ligase
RA 1,355,126 G→T 9.3% Q14K (CAG→AAG)  puuA ← glutamate‑‑putrescine ligase
RA 1,393,328 T→C 7.7% K152K (AAA→AAG fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator; autorepressor
RA 1,422,040 A→G 9.1% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 12.1% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 7.3% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 5.9% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,798 A→G 5.5% pseudogene (186/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,460,458 T→C 7.3% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,467 G→A 9.3% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,593 C→G 8.7% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,478,007 G→T 8.0% A230A (GCG→GCT hrpA → putative ATP‑dependent helicase
RA 1,579,178 C→A 8.7% A522A (GCG→GCT ydeP ← putative oxidoreductase
RA 1,637,078 C→A 9.2% E141* (GAA→TAA)  quuQ ← Qin prophage; putative antitermination protein Q
RA 1,673,251 G→A 10.0% A190T (GCA→ACA)  ydgH → DUF1471 family periplasmic protein
RA 1,679,434 G→T 16.7% V307F (GTT→TTT)  tus → inhibitor of replication at Ter, DNA‑binding protein
RA 1,705,966 G→T 9.7% A63S (GCG→TCG)  nth → DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
RA 1,788,668 C→A 7.7% A248S (GCG→TCG)  btuC ← vitamin B12 transporter subunit: membrane component of ABC superfamily
RA 1,807,926 G→A 14.8% intergenic (‑6/‑198) cedA ← / → katE cell division modulator/catalase HPII, heme d‑containing
RA 1,810,481 T→A 12.5% intergenic (+96/+162) katE → / ← chbG catalase HPII, heme d‑containing/chito‑oligosaccharide deacetylase
RA 1,862,280 C→T 7.3% R372R (CGC→CGT yeaG → protein kinase, endogenous substrate unidentified; autokinase
RA 1,891,649 Δ1 bp 5.8% coding (461/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,891,650 Δ1 bp 5.8% coding (462/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,891,652 Δ1 bp 5.8% coding (464/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,891,653 Δ1 bp 5.9% coding (465/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,891,654 Δ1 bp 5.8% coding (466/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,891,655 Δ1 bp 5.8% coding (467/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,891,660 Δ1 bp 5.7% coding (472/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,891,661 Δ1 bp 5.7% coding (473/1365 nt) sdaA → L‑serine dehydratase 1
RA 1,903,899 G→T 6.5% A153E (GCA→GAA)  kdgR ← KDG regulon transcriptional repressor
RA 1,915,625 G→T 14.7% R382R (CGG→CGT rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,922,095 C→T 6.0% Q334Q (CAG→CAA ptrB ← protease II
RA 1,924,637 C→A 12.3% R4I (AGA→ATA)  yebF ← extracellular Colicin M immunity family protein
RA 2,044,882 C→A 9.3% T2155K (ACA→AAA)  yeeJ → putative adhesin
RA 2,061,641 G→T 6.0% intergenic (‑268/+101) yoeG ← / ← yoeH pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,110,046 C→A 12.0% R188M (AGG→ATG)  wcaL ← putative glycosyl transferase
RA 2,112,103 A→G 7.6% intergenic (‑218/+58) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,118,284:1 +C 11.8% coding (944/1224 nt) wcaI ← putative glycosyl transferase
RA 2,118,284:2 +G 11.8% coding (944/1224 nt) wcaI ← putative glycosyl transferase
RA 2,118,289:1 +G 12.1% coding (939/1224 nt) wcaI ← putative glycosyl transferase
RA 2,118,289:2 +G 12.1% coding (939/1224 nt) wcaI ← putative glycosyl transferase
RA 2,118,289:3 +A 12.1% coding (939/1224 nt) wcaI ← putative glycosyl transferase
RA 2,118,289:4 +G 12.1% coding (939/1224 nt) wcaI ← putative glycosyl transferase
RA 2,118,293 T→A 12.1% Q312L (CAA→CTA) ‡ wcaI ← putative glycosyl transferase
RA 2,118,294 G→T 12.0% Q312K (CAA→AAA) ‡ wcaI ← putative glycosyl transferase
RA 2,118,295 C→T 12.3% V311V (GTG→GTA) ‡ wcaI ← putative glycosyl transferase
RA 2,118,296 A→G 12.1% V311A (GTG→GCG) ‡ wcaI ← putative glycosyl transferase
RA 2,118,298 C→G 12.9% V310V (GTG→GTC) ‡ wcaI ← putative glycosyl transferase
RA 2,118,299 A→T 12.9% V310E (GTG→GAG) ‡ wcaI ← putative glycosyl transferase
RA 2,118,300 C→A 12.9% V310L (GTG→TTG) ‡ wcaI ← putative glycosyl transferase
RA 2,118,301 C→T 12.9% L309L (CTG→CTA) ‡ wcaI ← putative glycosyl transferase
RA 2,118,303 G→C 12.9% L309V (CTG→GTG) ‡ wcaI ← putative glycosyl transferase
RA 2,118,304 A→C 12.9% H308Q (CAT→CAG) ‡ wcaI ← putative glycosyl transferase
RA 2,118,305 T→G 12.9% H308P (CAT→CCT) ‡ wcaI ← putative glycosyl transferase
RA 2,120,184 G→A 6.1% P163L (CCG→CTG)  wcaG ← bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase
RA 2,146,958 A→G 10.4% intergenic (+26/‑167) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
RA 2,146,979 C→A 6.4% intergenic (+47/‑146) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
RA 2,146,982 A→C 7.2% intergenic (+50/‑143) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
RA 2,146,984 T→A 8.5% intergenic (+52/‑141) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
RA 2,147,005 G→A 9.9% intergenic (+73/‑120) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
RA 2,147,006 A→G 8.1% intergenic (+74/‑119) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
RA 2,174,546 G→T 5.2% A325S (GCG→TCG)  yegU → ADP‑ribosylglycohydrolase family protein
RA 2,202,609 C→A 5.1% pseudogene (31/2001 nt) yehQ → pseudogene
RA 2,202,686 C→A 5.8% pseudogene (108/2001 nt) yehQ → pseudogene
RA 2,233,446 C→A 10.1% A128S (GCC→TCC)  mglB ← methyl‑galactoside transporter subunit
RA 2,234,573 G→T 8.6% A192D (GCC→GAC)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
RA 2,298,241 T→C 11.0% intergenic (+369/+346) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,492 A→G 8.5% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,498 T→C 6.5% intergenic (+626/+89) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,313,175 G→T 8.0% R61S (CGC→AGC)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,317,760 C→T 9.3% A58V (GCG→GTG)  atoA → acetyl‑CoA:acetoacetyl‑CoA transferase, beta subunit
RA 2,326,347 C→A 8.9% pseudogene (1535/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,337,427 G→T 5.6% A74A (GCC→GCA yfaL ← adhesin
RA 2,339,769 G→T 5.9% D476Y (GAT→TAT)  nrdA → ribonucleoside‑diphosphate reductase 1, alpha subunit
RA 2,362,727 C→A 5.9% R404S (CGC→AGC)  arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
RA 2,368,831 C→A 5.1% R204L (CGT→CTT)  menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,386,895 A→C 5.8% L544R (CTG→CGG)  nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L
RA 2,399,955 C→A 84.3% E56* (GAA→TAA)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,447,549 T→C 5.7% pseudogene (932/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
RA 2,452,532 G→T 6.3% A36D (GCC→GAC)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,594,224 G→T 5.4% G130C (GGC→TGC)  bcp → peroxiredoxin; thiol peroxidase, thioredoxin‑dependent
RA 2,615,831 C→T 7.5% P80L (CCC→CTC)  purN → phosphoribosylglycinamide formyltransferase 1
RA 2,674,989 C→T 5.6% L372L (CTG→CTA yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,677,535 G→T 11.5% intergenic (+120/+78) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,701,429 G→A 6.9% Q229* (CAG→TAG)  rseB ← anti‑sigma E factor, binds RseA
RA 2,711,646 G→A 10.3% P81L (CCG→CTG)  yfiF ← putative methyltransferase
RA 2,719,365 A→C 12.7% intergenic (‑260/+63) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,719,426 T→C 12.5% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,745,804 G→T 6.6% L217L (CTG→CTT recN → recombination and repair protein
RA 2,769,317 G→C 7.3% G14R (GGA→CGA)  yfjX → CP4‑57 prophage; putative antirestriction protein
RA 2,775,485 G→T 6.5% Q201K (CAG→AAG)  ypjA ← adhesin‑like autotransporter
RA 2,776,157 A→G 8.7% intergenic (‑72/+267) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family
RA 2,794,395 C→A 10.8% P24P (CCC→CCA nrdI → NrdEF cluster assembly flavodoxin
RA 2,796,461 G→T 5.1% A585A (GCG→GCT nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit
RA 2,798,218 C→A 7.8% G15G (GGC→GGA proV → glycine betaine transporter subunit
RA 2,813,783 C→A 5.9% A530S (GCG→TCG)  alaS ← alanyl‑tRNA synthetase
RA 2,813,829 G→T 7.0% G514G (GGC→GGA alaS ← alanyl‑tRNA synthetase
RA 2,823,524 C→A 11.8% A118E (GCG→GAG)  srlQ → D‑arabinose 5‑phosphate isomerase
RA 2,884,981 C→A 15.9% V93F (GTT→TTT)  cysJ ← sulfite reductase, alpha subunit, flavoprotein
RA 2,889,634 G→A 6.1% A94V (GCG→GTG)  ygcR ← putative flavoprotein
RA 2,917,469 T→C 9.8% T2A (ACC→GCC)  yqcC ← DUF446 family protein
RA 2,920,125 C→A 5.3% C153* (TGC→TGA ygdH → UPF0717 family protein
RA 2,933,427 G→A 6.4% R234H (CGC→CAC)  fucR → l‑fucose operon activator
RA 2,963,345 G→T 9.8% E109* (GAA→TAA)  mutH → methyl‑directed mismatch repair protein
RA 3,000,647 G→A 8.7% G343S (GGT→AGT)  ygeW → putative carbamoyltransferase
RA 3,015,327 T→A 6.2% L218Q (CTG→CAG)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
RA 3,143,950 C→A 13.1% intergenic (+45/+227) yghA → / ← exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
RA 3,152,391 C→A 6.6% A705A (GCG→GCT ygiQ ← Radical SAM superfamily protein
RA 3,157,477 T→A 8.3% K619M (AAG→ATG)  parC ← DNA topoisomerase IV, subunit A
RA 3,173,939 G→A 6.8% E54K (GAA→AAA)  ygiC → ATP‑Grasp family ATPase
RA 3,173,943 T→G 6.6% L55R (CTG→CGG)  ygiC → ATP‑Grasp family ATPase
RA 3,173,946 Δ1 bp 6.2% coding (167/1161 nt) ygiC → ATP‑Grasp family ATPase
RA 3,174,148 A→G 9.2% S123S (TCA→TCG ygiC → ATP‑Grasp family ATPase
RA 3,189,615 C→T 5.4% L166L (CTG→CTA hldE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
RA 3,216,152 A→G 7.6% D54G (GAC→GGC)  ebgA → evolved beta‑D‑galactosidase, alpha subunit
RA 3,225,833 C→T 5.7% S270S (AGC→AGT fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,231,877 C→A 8.3% intergenic (+221/‑62) ygjR → / → alx putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
RA 3,242,439 G→A 7.3% A38T (GCC→ACC)  yqjC → DUF1090 family putative periplasmic protein
RA 3,310,696 Δ1 bp 5.5% coding (190/1488 nt) nusA ← transcription termination/antitermination L factor
RA 3,310,697 Δ1 bp 5.5% coding (189/1488 nt) nusA ← transcription termination/antitermination L factor
RA 3,310,698 Δ1 bp 5.5% coding (188/1488 nt) nusA ← transcription termination/antitermination L factor
RA 3,310,699 Δ1 bp 5.5% coding (187/1488 nt) nusA ← transcription termination/antitermination L factor
RA 3,310,700 Δ1 bp 5.5% coding (186/1488 nt) nusA ← transcription termination/antitermination L factor
RA 3,330,960 G→A 6.6% F97F (TTC→TTT mlaC ← ABC transporter maintaining OM lipid asymmetry, periplasmic binding protein
RA 3,334,296 C→A 5.9% S221R (AGC→AGA yrbG → putative calcium/sodium:proton antiporter
RA 3,338,859 C→A 6.7% Q262K (CAG→AAG)  rpoN → RNA polymerase, sigma 54 (sigma N) factor
RA 3,356,501 C→A 6.7% S112S (TCC→TCA yhcD → putative outer membrane fimbrial subunit usher protein
RA 3,390,542 T→C 5.8% T205A (ACC→GCC)  rng ← ribonuclease G
RA 3,407,558 G→T 7.5% A112A (GCG→GCT acrE → cytoplasmic membrane lipoprotein
RA 3,408,099 C→T 6.4% R293C (CGT→TGT)  acrE → cytoplasmic membrane lipoprotein
RA 3,408,967 G→A 6.8% L192L (CTG→CTA acrF → multidrug efflux system protein
RA 3,414,397 A→G 5.5% N302S (AAC→AGC)  yhdX → putative amino‑acid transporter subunit
RA 3,416,890 T→G 8.6% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 11.3% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,421,849 T→A 5.8% noncoding (273/1542 nt) rrsD ← 16S ribosomal RNA of rrnD operon
RA 3,464,590 G→A 5.4% T33T (ACC→ACT tufA ← translation elongation factor EF‑Tu 1
RA 3,530,283 G→T 6.9% S38I (AGT→ATT)  greB → transcript cleavage factor
RA 3,541,739 C→G 9.5% G564A (GGC→GCC)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,556,972 T→C 6.2% intergenic (+94/+175) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
RA 3,556,973 G→A 6.2% intergenic (+95/+174) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
RA 3,556,976 T→A 6.1% intergenic (+98/+171) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
RA 3,556,977 T→C 6.1% intergenic (+99/+170) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
RA 3,556,978 A→C 5.3% intergenic (+100/+169) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
RA 3,562,212 T→C 8.3% K159K (AAA→AAG glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,567,582 G→T 5.4% G219G (GGC→GGA asd ← aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding
RA 3,576,465 C→A 18.1% pseudogene (68/381 nt) yrhA → pseudogene, interrupted by IS1E
RA 3,604,180 C→A 7.0% W305L (TGG→TTG)  yhhS ← putative arabinose efflux transporter
RA 3,612,581 T→C 7.1% G10G (GGT→GGC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,616,121 G→C 6.8% R1190R (CGG→CGC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,632,564 Δ1 bp 8.6% intergenic (+63/+181) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
RA 3,643,118 T→A 14.3% L275Q (CTG→CAG)  arsB → arsenite/antimonite transporter
RA 3,649,097 C→A 6.0% V56L (GTG→TTG)  yhiD ← putative Mg(2+) transport ATPase, inner membrane protein
RA 3,655,168 G→T 5.3% E465D (GAG→GAT mdtF → anaerobic multidrug efflux transporter, ArcA‑regulated
RA 3,663,838 G→A 5.3% W296* (TGG→TAG)  treF → cytoplasmic trehalase
RA 3,664,466 C→T 8.2% H505H (CAC→CAT treF → cytoplasmic trehalase
RA 3,686,614 C→T 5.0% P661P (CCG→CCA bcsA ← cellulose synthase, catalytic subunit
RA 3,734,745 G→A 9.3% C66C (TGC→TGT ysaA ← putative hydrogenase, 4Fe‑4S ferredoxin‑type component
RA 3,750,722 C→A 8.1% A156S (GCG→TCG)  yiaY ← putative Fe‑containing alcohol dehydrogenase, Pfam00465 family
RA 3,768,250 C→A 7.4% A156D (GCC→GAC)  mtlD → mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
RA 3,768,608 G→T 5.7% Q275H (CAG→CAT mtlD → mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
RA 3,886,383 G→T 5.5% D87Y (GAT→TAT)  yidZ → putative DNA‑binding transcriptional regulator
RA 3,935,371 G→T 7.0% noncoding (204/1542 nt) rrsC → 16S ribosomal RNA of rrnC operon
RA 3,965,897 A→G 8.0% I6V (ATA→GTA)  rffG → dTDP‑glucose 4,6‑dehydratase
RA 3,972,523 G→A 5.4% S188N (AGC→AAC)  wzyE → putative ECA polysaccharide chain elongation protein
RA 4,037,083 C→A 9.4% G102G (GGC→GGA dsbA → periplasmic protein disulfide isomerase I
RA 4,041,921 G→T 6.2% P532P (CCG→CCT polA → fused DNA polymerase I 5'‑>3' polymerase/3'‑>5' exonuclease/5'‑>3' exonuclease
RA 4,047,523 G→A 7.4% A372A (GCC→GCT glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
RA 4,049,683 T→C 6.6% Q6R (CAG→CGG)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,073,367 G→A 5.0% intergenic (+38/+292) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,368 C→G 5.3% intergenic (+39/+291) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,371 G→T 6.5% intergenic (+42/+288) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,417 G→A 15.0% intergenic (+88/+242) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,418 T→A 6.8% intergenic (+89/+241) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,420 G→A 12.1% intergenic (+91/+239) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,422 A→G 18.6% intergenic (+93/+237) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,423 T→C 18.6% intergenic (+94/+236) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,433 G→A 14.1% intergenic (+104/+226) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,073,434 T→G 9.6% intergenic (+105/+225) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
RA 4,082,032 G→A 10.5% P395L (CCG→CTG)  frvR ← putative frv operon regulator; contains a PTS EIIA domain
RA 4,088,923 G→A 7.8% R117H (CGC→CAC)  rhaR → transcriptional activator of rhaSR
RA 4,133,261 C→A 5.3% A268A (GCC→GCA frwC → putative enzyme IIC component of PTS
RA 4,151,394 G→A 8.9% G115S (GGC→AGC) ‡ fabR → transcriptional repressor of fabA and fabB
RA 4,151,395 G→C 9.0% G115A (GGC→GCC) ‡ fabR → transcriptional repressor of fabA and fabB
RA 4,165,449 C→T 30.7% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 30.7% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,173,307 C→T 24.0% S712F (TCC→TTC)  rpoB → RNA polymerase, beta subunit
RA 4,177,142 A→G 62.9% D622G (GAC→GGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,192,224 T→C 6.8% L124S (TTA→TCA)  zraS → sensory histidine kinase in two‑component regulatory system with ZraR
RA 4,285,970 C→T 6.4% intergenic (+248/+283) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,315,882 A→G 12.3% intergenic (‑324/+334) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,908 T→C 11.1% intergenic (‑350/+308) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,336,238 A→G 7.1% R302R (CGT→CGC fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,241 G→A 7.4% I301I (ATC→ATT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,363,262 G→T 10.7% L264I (CTA→ATA)  yjeJ ← uncharacterized protein
RA 4,370,239 G→A 10.0% D32D (GAC→GAT frdB ← fumarate reductase (anaerobic), Fe‑S subunit
RA 4,381,709 G→A 6.7% V97M (GTG→ATG)  orn → oligoribonuclease
RA 4,399,063 C→A 7.2% intergenic (+151/‑29) rnr → / → rlmB exoribonuclease R, RNase R/23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,436,100 A→G 6.1% E724E (GAA→GAG tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,436,934 C→T 6.2% G1002G (GGC→GGT tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,467,913 A→C 10.0% A72A (GCT→GCG argI ← ornithine carbamoyltransferase 1
RA 4,467,949 T→A 6.0% R60R (CGA→CGT argI ← ornithine carbamoyltransferase 1
RA 4,473,074 T→C 10.0% K194R (AAA→AGA)  valS ← valyl‑tRNA synthetase
RA 4,479,271 G→T 6.2% L204M (CTG→ATG)  yjgR ← DUF853 family protein with NTPase fold
RA 4,508,444 C→A 8.2% P34H (CCT→CAT) ‡ insA → IS1 repressor TnpA
RA 4,508,445 T→C 7.0% P34P (CCT→CCC) ‡ insA → IS1 repressor TnpA
RA 4,508,466 A→G 5.3% L41L (CTA→CTG insA → IS1 repressor TnpA
RA 4,599,731 G→A 9.3% A167A (GCG→GCA prfC → peptide chain release factor RF‑3
RA 4,604,320 T→C 10.8% intergenic (+243/+177) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,362 G→A 13.0% intergenic (+285/+135) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,604,365 T→C 13.2% intergenic (+288/+132) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,623,497 G→T 9.8% H22Q (CAC→CAA yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 959602 959612 11 6 [4] [0] 18 ihfB/ycaI integration host factor (IHF), DNA‑binding protein, beta subunit/ComEC family inner membrane protein
* * ÷ CP009273 2050062 2050299 238 6 [1] [3] 6 amn/yeeN AMP nucleosidase/UPF0082 family protein
* * ÷ CP009273 2208333 2209059 727 9 [4] [4] 7 [mlrA] [mlrA]
* * ÷ CP009273 2462840 2463098 259 6 [3] [5] 6 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2463615 2463821 207 9 [4] [5] 6 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 3406947 3406991 45 10 [1] [4] 6 acrS/acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
* * ÷ CP009273 3797707 3797781 75 8 [4] [4] 7 waaS lipopolysaccharide core biosynthesis protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 335746 =100 (1.710)22 (0.380) 8/246 NT 18.2% noncoding (279/358 nt) REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences
?CP009273 372778 = NA (NA)intergenic (+11/+213) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 360104 =38 (0.650)46 (0.790) 19/248 NT 54.8% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370386 =NA (NA)11 (0.290) 5/162 NT 22.1% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372978 = 43 (1.010)intergenic (+211/+13) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 1203246 =31 (0.530)10 (0.200) 6/216 NT 32.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 15 (0.290)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326129 (0.500)23 (0.450) 10/216 NT 53.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 15 (0.290)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1972762 =36 (0.620)8 (0.140) 3/248 NT 18.2% intergenic (‑308/‑472) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region