breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA CP009273 164,770 +G coding (800/2244 nt) fhuA → ferrichrome outer membrane transporter
JC CP009273 1,257,022 (AGTGATTGGTACACGAGC)1→2 coding (310/948 nt) prs ← phosphoribosylpyrophosphate synthase
MC JC CP009273 2,400,028 Δ9 bp coding (85‑93/939 nt) lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA CP009273 2,528,814 G→A D464N (GAC→AAC)  ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)
MC JC CP009273 2,897,462 Δ61 bp intergenic (+729/+584) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA CP009273 3,051,140 A→T H210Q (CAT→CAA serA ← D‑3‑phosphoglycerate dehydrogenase
RA CP009273 3,480,047 T→A M190K (ATG→AAG)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression
RA CP009273 3,547,392 T→A W317R (TGG→AGG)  malT → mal regulon transcriptional activator
RA CP009273 3,723,336 T→G K264Q (AAA→CAA)  xylA ← D‑xylose isomerase
RA CP009273 3,907,225 +A coding (1336/1371 nt) glmU ← fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
RA CP009273 4,151,176 T→G V42G (GTC→GGC)  fabR → transcriptional repressor of fabA and fabB
RA CP009273 4,154,051 G→C G162A (GGA→GCA)  btuB → vitamin B12/cobalamin outer membrane transporter
RA CP009273 4,224,817 G→T G378C (GGT→TGT)  pgi → glucosephosphate isomerase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 782240 783098 859 100 [98] [97] 100 gpmA gpmA
* * ÷ CP009273 1254187 1254572 386 100 [98] [98] 102 ychH ychH
* * ÷ CP009273 1781647 1782586 940 100 [98] [99] 100 ppsR ppsR
* * ÷ CP009273 1800564 1801601 1038 101 [98] [99] 100 pfkB pfkB
* * ÷ CP009273 1929062 1930615 1554 102 [99] [96] 100 zwf zwf
* * ÷ CP009273 3778559 3780215 1657 100 [99] [99] 100 gpmM–[envC] gpmM,[envC]
* * ÷ CP009273 4097427 4098475 1049 105 [99] [98] 100 pfkA pfkA
* * ÷ CP009273 4205384 4210272 4889 100 [97] [99] 100 aceB–[arpA] aceB,aceA,aceK,[arpA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =148 (0.880)159 (0.990)
+AATTCC
99/238 0.9 52.2% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 4033989 = 158 (0.940)noncoding (113/120 nt) rrfA 5S ribosomal RNA of rrnA operon