breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | 100% | [crl] | [crl] | |
RA | 759,544 | A→G | 9.7% | D280G (GAC→GGC) | sucA → | 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding |
RA | 1,068,294 | C→G | 8.7% | intergenic (+40/+217) | ymdF → / ← rutG | KGG family protein/pyrimidine permease |
RA | 1,413,542 | A→T | 100% | Y67N (TAT→AAT) | ydaC ← | DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein |
RA | 1,535,527 | Δ1 bp | 17.6% | coding (332/894 nt) | yddE ← | PhzC‑PhzF family protein |
RA | 1,793,897 | Δ1 bp | 100% | coding (213/552 nt) | btuE ← | glutathione peroxidase |
RA | 1,825,023 | T→A | 11.0% | intergenic (‑86/+117) | ves ← / ← spy | cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced |
MC JC | 1,978,503 | Δ776 bp | 100% | insB1–insA | insB1, insA | |
RA | 2,045,098 | Δ1 bp | 14.3% | coding (161/7077 nt) | yeeJ → | putative adhesin |
RA | 2,045,099 | Δ1 bp | 14.3% | coding (162/7077 nt) | yeeJ → | putative adhesin |
RA | 2,050,073 | C→T | 8.0% | S1712S (AGC→AGT) | yeeJ → | putative adhesin |
RA | 2,093,040 | G→A | 100% | A215T (GCT→ACT) | hisC → | histidinol‑phosphate aminotransferase |
RA | 2,876,477 | C→A | 10.9% | intergenic (‑150/‑102) | cysD ← / → iap | sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion |
RA | 3,223,086 | G→T | 6.3% | K152N (AAG→AAT) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
JC | 3,972,029 | +CCTGGC | 26.8% | coding (771/1263 nt) | wecC → | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
RA | 3,975,297 | Δ1 bp | 69.4% | coding (153/1131 nt) | wecE → | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
RA | 3,975,298 | Δ1 bp | 70.6% | coding (154/1131 nt) | wecE → | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
RA | 4,117,004 | T→A | 100% | D73V (GAT→GTT) | glpK ← | glycerol kinase |
MC JC | 4,188,484 | Δ27 bp | 100% | coding (3136‑3162/4224 nt) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,508,694 | A→T | 14.3% | pseudogene (250/267 nt) | insM ← | pseudogene, IS600 transposase;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 366210 | 366403 | 194 | 11 [10] | [10] 11 | [lacZ] | [lacZ] |
* | * | ÷ | NC_000913 | 3423753–3424232 | 3424521–3424236 | 5–769 | 11 [10] | [10] 11 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 16 (0.330) | 18 (0.420) | 17/244 | NT | 51.3% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 20 (0.470) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 16 (0.330) | 15 (0.350) | 15/244 | NT | 46.8% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 20 (0.470) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 52 (1.110) | 43/268 | NT | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |