breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 700,948 | A→C | I216S (ATT→AGT) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 2,509,469 | G→A | intergenic (‑45/‑159) | glk ← / → yfeO | glucokinase/putative ion channel protein |
RA | 2,977,578 | T→G | S328A (TCA→GCA) | galR → | galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor |
RA | 3,267,595 | A→G | *73* (TAA→TGA) | tdcR → | L‑threonine dehydratase operon activator protein |
RA | 3,769,672 | (A)8→7 | pseudogene (1/111 nt) | yibV → | pseudogene, putative cryptic Rhs immunity protein |
MC JC | 3,815,858 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
JC | 4,001,644 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,184,542 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 2533709 | 2536360 | 2652 | 26 [24] | [25] 26 | ptsH–crr | ptsH, ptsI, crr |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 257908 = | NA (NA) | 74 (1.220) | 57/282 | 0.0 | 97.4% | intergenic (+9/‑768) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | = 1293039 | 2 (0.030) | intergenic (‑117/‑488) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase | |||||
* | ? | NC_000913 | 1207790 = | 12 (0.200) | 35 (0.650) | 30/250 | 0.9 | 73.2% | coding (290/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | 1209619 = | 15 (0.280) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 11 (0.180) | 38 (0.710) | 27/250 | 1.2 | 75.4% | coding (305/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | = 1209602 | 15 (0.280) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | 1293032 = | 2 (0.030) | 58 (0.960) | 46/282 | 0.2 | 96.7% | intergenic (‑110/‑495) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
? | NC_000913 | = 1979270 | NA (NA) | intergenic (‑55/‑483) | insA/uspC | IS1 repressor TnpA/universal stress protein | |||||
* | ? | NC_000913 | = 1299498 | 1 (0.020) | 57 (0.970) | 48/274 | 0.1 | 99.2% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 1979486 = | 3 (0.050) | 95 (1.570) | 65/282 | 0.0 | 96.9% | intergenic (‑271/‑267) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | 2101749 = | NA (NA) | intergenic (‑5/+146) | wbbL/insH1 | pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator | |||||
* | ? | NC_000913 | = 1979489 | 1 (0.020) | 63 (1.040) | 46/282 | 0.2 | 98.4% | intergenic (‑274/‑264) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | = 2290111 | NA (NA) | intergenic (‑32/+1) | insH1/yejO | IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system | |||||
* | ? | NC_000913 | 2288917 = | NA (NA) | 53 (0.870) | 42/282 | 0.4 | 100% | intergenic (‑5/+146) | yejO/insH1 | pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system/IS5 transposase and trans‑activator |
? | NC_000913 | 3526447 = | 0 (0.000) | intergenic (‑300/‑20) | nudE/yrfF | adenosine nucleotide hydrolase, Ap3A/Ap2A/ADP‑ribose/NADH hydrolase/putative RcsCDB‑response attenuator, inner membrane protein | |||||
* | ? | NC_000913 | = 2290110 | NA (NA) | 91 (1.510) | 52/280 | 0.1 | 98.9% | intergenic (‑31/+2) | insH1/yejO | IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |
? | NC_000913 | = 3526451 | 1 (0.020) | intergenic (‑304/‑16) | nudE/yrfF | adenosine nucleotide hydrolase, Ap3A/Ap2A/ADP‑ribose/NADH hydrolase/putative RcsCDB‑response attenuator, inner membrane protein | |||||
* | ? | NC_000913 | 2313534 = | 4 (0.070) | 57 (0.970) | 42/274 | 0.3 | 93.6% | coding (49/2673 nt) | rcsD | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
? | NC_000913 | = 2515611 | NA (NA) | intergenic (+170/+30) | insL1/yfeA | IS186 transposase/putative diguanylate cyclase | |||||
* | ? | NC_000913 | = 2313545 | 3 (0.050) | 50 (0.840) | 44/278 | 0.3 | 94.4% | coding (60/2673 nt) | rcsD | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
? | NC_000913 | 2514275 = | NA (NA) | intergenic (+33/‑54) | nupC/insL1 | nucleoside (except guanosine) transporter/IS186 transposase | |||||
* | ? | NC_000913 | 3845530 = | 47 (0.780) | 58 (1.030) | 51/262 | 0.1 | 57.2% | coding (1568/1767 nt) | adeD | cryptic adenine deaminase |
? | NC_000913 | = 3992759 | 43 (0.760) | coding (1608/2547 nt) | cyaA | adenylate cyclase |