breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 555,478 | C→A | R290S (CGT→AGT) | cysS → | cysteinyl‑tRNA synthetase |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
RA | 1,196,245 | T→C | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
RA | 1,196,247 | A→G | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
JC JC | 1,293,032 | IS1 (–) +8 bp | intergenic (‑110/‑488) | hns ← / → tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
MC JC | 1,299,499 | Δ1,199 bp | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
RA | 1,806,340 | C→T | intergenic (‑257/‑30) | ydiY ← / → pfkB | acid‑inducible putative outer membrane protein/6‑phosphofructokinase II |
RA | 1,807,117 | A→G | S250G (AGT→GGT) | pfkB → | 6‑phosphofructokinase II |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,661,691 | C→A | A147A (GCG→GCT) | iscR ← | isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor |
MC JC | 3,559,357 | Δ1,629 bp | [rtcR]–[glpG] | [rtcR], glpR, glpR, [glpG] | |
MC JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
MC JC | 4,001,645 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,184,543 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1196263 | 1211427 | 15165 | 9 [8] | [8] 9 | [icd]–[icdC] | [icd],ymfD,ymfE,lit,intE,xisE,ymfI,ymfJ,cohE,croE,ymfL,ymfM,oweE,aaaE,ymfR,beeE,jayE,ymfQ,stfP,tfaP,tfaE,stfE,pinE,mcrA,[icdC] |
* | * | ÷ | NC_000913 | 2066286–2066577 | 2066577 | 1–292 | 10 [8] | [7] 9 | [insH1] | [insH1] |
* | * | ÷ | NC_000913 | 3423765–3424234 | 3424547–3424238 | 5–783 | 9 [8] | [8] 9 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
* | * | ÷ | NC_000913 | 4107543 | 4108522 | 980 | 9 [8] | [8] 9 | pfkA | pfkA |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 3423376 = | 36 (1.040) | 13 (0.400) +TTGATGT |
11/232 | 2.0 | 31.1% | intergenic (+182/+47) | yhdZ/rrfF | putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon |
? | NC_000913 | = 4213155 | 25 (0.720) | noncoding (116/120 nt) | rrfE | 5S ribosomal RNA of rrnE operon |