breseq version 0.32.0b
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 982,560 | T→A | intergenic (‑122/+481) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
RA | 1,191,676 | C→T | H366H (CAC→CAT) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,688 | C→T | T370T (ACC→ACT) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,701 | T→C | L375M (TTA→CTG) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,703 | A→G | L375M (TTA→CTG) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,733 | C→T | N385N (AAC→AAT) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,736 | G→C | A386A (GCG→GCC) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,739 | A→G | K387K (AAA→AAG) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,748 | C→T | T390T (ACC→ACT) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 2,083,992 | C→A | S107* (TCG→TAG) | hisG → | ATP phosphoribosyltransferase |
JC JC | 2,400,257 | IS5 (+) +4 bp | intergenic (‑137/‑780) | lrhA ← / → alaA | transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2 |
RA | 2,420,328 | G→A | intergenic (‑61/+160) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
JC | 2,420,336 | +AAAA | intergenic (‑69/+152) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
RA | 2,987,946 | G→A | pseudogene (506/633 nt) | ygeK ← | pseudogene; putative 2‑component transcriptional regulator |
JC JC | 3,178,254 | IS5 (+) +4 bp | coding (56‑59/291 nt) | yqiC → | BMFP family putative fusogenic protein |
RA | 3,370,282 | C→T | W166* (TGG→TGA) | sspA ← | stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog |
RA | 3,433,555 | G→T | I278I (ATC→ATA) | rpoA ← | RNA polymerase, alpha subunit |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | CP009273 | 1191755 | 1206909 | 15155 | 16 [15] | [15] 17 | [icd]–[icdC] | 25 genes [icd], ymfD, ymfE, lit, intE, xisE, ymfI, ymfJ, cohE, croE, ymfL, ymfM, oweE, aaaE, ymfR, beeE, jayE, ymfQ, stfP, tfaP, tfaE, stfE, pinE, mcrA, [icdC] |
* | * | ÷ | CP009273 | 2084540 | 2085892 | 1353 | 17 [15] | [13] 16 | [hisG]–[hisC] | [hisG], hisD, [hisC] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 313710 = | 2 (0.030) | 54 (0.920) | 43/296 | 0.3 | 96.4% | coding (321/594 nt) | rclC | reactive chlorine species (RCS) stress resistance inner membrane protein |
? | CP009273 | 1462167 = | NA (NA) | noncoding (1331/1331 nt) | IS2 | repeat region | |||||
* | ? | CP009273 | = 313714 | 1 (0.020) | 49 (0.840) | 42/296 | 0.4 | 98.0% | coding (317/594 nt) | rclC | reactive chlorine species (RCS) stress resistance inner membrane protein |
? | CP009273 | 376716 = | NA (NA) | intergenic (‑1/‑91) | yaiX/insC1 | pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA | |||||
* | ? | CP009273 | 360104 = | NA (NA) | 94 (1.600) | 69/296 | 0.0 | NA | intergenic (‑38/+557) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
? | CP009273 | = 360286 | NA (NA) | intergenic (‑220/+375) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |