breseq version 0.32.0b
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 273,525 | T→C | 17.5% | E330G (GAG→GGG) | afuC ← | CP4‑6 prophage; putative ferric transporter subunit |
JC JC | 542,271 | IS5 (–) +4 bp | 35.0% | coding (136‑139/1668 nt) | fdrA → | putative NAD(P)‑binding acyl‑CoA synthetase |
RA | 821,679 | C→T | 18.4% | G344R (GGG→AGG) | ybhS ← | inner membrane putative ABC superfamily transporter permease |
RA | 1,191,676 | C→T | 58.7% | H366H (CAC→CAT) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,688 | C→T | 50.5% | T370T (ACC→ACT) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,191,701 | T→C | 41.9% | L375L (TTA→CTA) | icd → | e14 prophage; isocitrate dehydrogenase, specific for NADP+ |
RA | 1,211,760 | T→C | 19.8% | S79S (AGT→AGC) | ymgA → | RcsB connector protein for regulation of biofilm |
RA | 1,268,411 | C→T | 17.4% | A150V (GCC→GTC) | chaC → | cation transport regulator |
JC JC | 2,400,257 | IS5 (+) +4 bp | 50.0% | intergenic (‑137/‑780) | lrhA ← / → alaA | transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2 |
JC | 2,420,337 | Δ120 bp | 77.4% | intergenic (‑70/+32) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
RA | 2,420,470 | C→A | 100% | intergenic (‑203/+18) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
RA | 2,719,426 | T→C | 40.7% | intergenic (‑321/+2) | kgtP ← / ← rrfG | alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
RA | 2,725,094 | A→G | 21.0% | G813G (GGT→GGC) | clpB ← | protein disaggregation chaperone |
JC JC | 3,178,254 | IS5 (+) +4 bp | 80.0% | coding (56‑59/291 nt) | yqiC → | BMFP family putative fusogenic protein |
RA | 3,180,810 | G→A | 16.8% | pseudogene (31/2445 nt) | yqiG → | pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein |
RA | 3,985,522 | T→C | 17.5% | L337P (CTC→CCC) | cyaA → | adenylate cyclase |
RA | 4,185,493 | C→G | 24.9% | E179Q (GAG→CAG) | thiC ← | phosphomethylpyrimidine synthase |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 360104 = | NA (NA) | 30 (1.690) | 22/276 | NT | NA | intergenic (‑38/+557) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
? | CP009273 | = 360286 | NA (NA) | intergenic (‑220/+375) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor | |||||
* | ? | CP009273 | 454243 = | 5 (0.280) | 6 (0.350) | 6/270 | NT | 55.1% | intergenic (+87/‑101) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | CP009273 | 603467 = | NA (NA) | intergenic (+23/‑54) | hokE/insL1 | toxic polypeptide, small/IS186 transposase | |||||
* | ? | CP009273 | = 454255 | 5 (0.280) | 5 (0.290) | 5/268 | NT | 50.7% | intergenic (+99/‑89) | clpX/lon | ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La |
? | CP009273 | = 604803 | NA (NA) | intergenic (+170/+112) | insL1/entD | IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex | |||||
* | ? | CP009273 | = 683479 | NA (NA) | 6 (0.350) | 6/266 | NT | 36.1% | noncoding (1023/1195 nt) | IS5 | repeat region |
? | CP009273 | 684510 = | 11 (0.620) | intergenic (‑41/+289) | insH1/lnt | IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase | |||||
* | ? | CP009273 | = 1391495 | NA (NA) | 9 (0.510) | 8/276 | NT | 52.9% | intergenic (+146/‑127) | insH1/ynaJ | IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein |
? | CP009273 | = 1972751 | 8 (0.450) | intergenic (‑297/‑483) | flhD/uspC | flagellar class II regulon transcriptional activator, with FlhC/universal stress protein | |||||
* | ? | CP009273 | 1972748 = | 9 (0.510) | 6 (0.340) | 4/274 | NT | 40.0% | intergenic (‑294/‑486) | flhD/uspC | flagellar class II regulon transcriptional activator, with FlhC/universal stress protein |
? | CP009273 | = 2283592 | NA (NA) | noncoding (1/1195 nt) | IS5 | repeat region | |||||
* | ? | CP009273 | 2269338 = | 14 (0.790) | 5 (0.280) | 5/276 | NT | 26.3% | coding (587/1026 nt) | yejE | microcin C transporter YejABEF, permease subunit; ABC family |
? | CP009273 | = 3577555 | NA (NA) | intergenic (+15/‑1) | insB1/yrhA | IS1 transposase B/pseudogene, interrupted by IS1E | |||||
* | ? | CP009273 | = 2269346 | 14 (0.790) | 13 (0.730) | 11/276 | NT | 48.1% | coding (595/1026 nt) | yejE | microcin C transporter YejABEF, permease subunit; ABC family |
? | CP009273 | 3576788 = | NA (NA) | intergenic (+10/‑55) | yrhA/insA | pseudogene, interrupted by IS1E/IS1 repressor TnpA | |||||
* | ? | CP009273 | 4532499 = | 8 (0.450) | 6 (0.360) | 5/258 | NT | 42.1% | intergenic (+49/‑433) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
? | CP009273 | 4532813 = | 9 (0.540) | intergenic (+363/‑119) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) | |||||
* | ? | CP009273 | = 4532507 | 7 (0.390) | 9 (0.540) | 7/258 | NT | 53.7% | intergenic (+57/‑425) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
? | CP009273 | = 4532803 | 9 (0.540) | intergenic (+353/‑129) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |