breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,934,582 A→G V8A (GTA→GCA)  edd ← 6‑phosphogluconate dehydratase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2066301–2066582 2066582 1–282 6 [5] [5] 6 [insH1] [insH1]
* * ÷ NC_000913 3423790–3424234 3424523–3424238 5–734 6 [4] [5] 6 [rrlD] [rrlD]
* * ÷ NC_000913 3514402 3515067 666 6 [5] [4] 6 [rpe] [rpe]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 225084NA (NA)12 (0.370) 10/242 1.8 NA noncoding (1314/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 225092 NA (NA)noncoding (1322/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 = 346823NA (NA)6 (0.180) 6/246 2.7 NA intergenic (+64/+34) yahO/prpR periplasmic protein, function unknown, YhcN family/propionate catabolism operon regulatory protein
?NC_000913 = 346853 NA (NA)noncoding (30/30 nt) REP24 (repetitive extragenic palindromic) element; contains 1 REP sequences REP24 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 381663 =NA (NA)5 (0.150) 5/242 3.0 NA noncoding (404/1331 nt) IS2 repeat region
?NC_000913 381703 = NA (NA)noncoding (444/1331 nt) IS2 repeat region